TLR2 Protein, Mouse (sf9, His)

The TLR2 protein cooperates with LY96 to initiate an immune response against bacterial lipoproteins and cell wall components. It cooperates with TLR1 or TLR6 to activate NF-kappa-B, induce cytokine secretion, and form activation clusters such as TLR2:TLR6:CD14:CD36. TLR2 Protein, Mouse (sf9, His) is the recombinant mouse-derived TLR2 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of TLR2 Protein, Mouse (sf9, His) is 587 a.a., with molecular weight of ~65 kDa.

TLR2 Protein collaborates with LY96 to initiate the innate immune response against bacterial lipoproteins and microbial cell wall components. Additionally, it cooperates with TLR1 or TLR6 for the response to specific lipopeptides, acting through MYD88 and TRAF6 to activate NF-kappa-B, induce cytokine secretion, and trigger inflammation. TLR2 forms activation clusters, like TLR2:TLR6:CD14:CD36 and TLR2:TLR1:CD14, in response to different ligands, leading to cell surface signaling and subsequent Golgi targeting via a lipid-raft dependent pathway. Notably, TLR2 recognizes M.tuberculosis antigens, including EsxA (ESAT-6), and serves as a major receptor for various M.tuberculosis lipoproteins, influencing antigen-presenting cell functions. Its interactions with TICAM2, LY96, TLR1, TLR6, MYD88, TICAM1, CNPY3, ATG16L1, non-modified M.tuberculosis protein MPT83, PPP1R11, and TIRAP contribute to its diverse roles in the immune response.

Mouse

Sf9 insect cells

C-His

Q9QUN7 (M1-Q587)

24088  [NCBI]

Approximately 65 kDa

Greater than 85% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.