Rhodamine B hydrazide

Cat. No.: HY-123645 Pureza: 99.87%: Ficha de datos
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Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu²⁺), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO).
Excitation/emission wavelength:
Conventional detection: 510/578 nm.
Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).

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No. CAS : 74317-53-6

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Rhodamine B hydrazide

•Chem Eng J. 2025 Sep 15.

Cell Imaging/Staining

: Rhodamine B hydrazide purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Sep 15.; CLSM images of copper ion uptake in MC38 cells after different treatments. Copper ions were stained with Rhodamine B hydrazide (RBH; 10 μM; 30 min).

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Revisión del cliente

Descripciòn

Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu²⁺), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO)^[1]^[2]^[3].
Excitation/emission wavelength:
Conventional detection: 510/578 nm.
Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).

In Vitro

Rhodamine B hydrazide is a colorless and non-fluorescent rhodamine derivative. While the emission is related to the concentration of sulfite (5-800 ng/mL; detection limit=1.4 ng/mL (3σ)). Sulfite reduces dissolved oxygen to yield superoxide radicals, which binds to Rhodamine B hydrazide to form Rhodamine B. Rhodamine B hydrazide gives Rhodamine B-like fluorescence in the presence of sulfite, which is enhanced by Tween 80 surfactant micelles. Rhodamine B hydrazide has an absorption maximum at 554 nm and a fluorescence emission maximum at 574 nm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

456.58

Fòrmula

C₂₈H₃₂N₄O₂

No. CAS

74317-53-6

Appearance

Solid

Color

Off-white to yellow

SMILES

CCN(C1=CC2=C(C3(C4=CC=C(C=C4O2)N(CC)CC)C5=C(C(N3N)=O)C=CC=C5)C=C1)CC

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (219.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1902 mL	10.9510 mL	21.9020 mL
5 mM	0.4380 mL	2.1902 mL	4.3804 mL
10 mM	0.2190 mL	1.0951 mL	2.1902 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

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Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.87%

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Ficha de datos (279 KB) SDS (393 KB)

COA (257 KB) HNMR (133 KB) RP-HPLC (226 KB) LCMS (97 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Yang X F, et al. Novel spectrofluorimetric method for the determination of sulfite with rhodamine B hydrazide in a micellar medium[J]. Analytica Chimica Acta, 2002, 456(1): 121-128.

[2]. Suresh Kumar Kempahanumakkagaari, et al. A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media. Journal of Luminescence Volume 146, February 2014, Pages 11-17.

[3]. Wu CM, et al. Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging. Anal Chim Acta. 2011 Dec 5;708(1-2):141-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1902 mL	10.9510 mL	21.9020 mL	54.7549 mL
	5 mM	0.4380 mL	2.1902 mL	4.3804 mL	10.9510 mL
	10 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4755 mL
	15 mM	0.1460 mL	0.7301 mL	1.4601 mL	3.6503 mL
	20 mM	0.1095 mL	0.5475 mL	1.0951 mL	2.7377 mL
	25 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	30 mM	0.0730 mL	0.3650 mL	0.7301 mL	1.8252 mL
	40 mM	0.0548 mL	0.2738 mL	0.5475 mL	1.3689 mL
	50 mM	0.0438 mL	0.2190 mL	0.4380 mL	1.0951 mL
	60 mM	0.0365 mL	0.1825 mL	0.3650 mL	0.9126 mL
	80 mM	0.0274 mL	0.1369 mL	0.2738 mL	0.6844 mL
	100 mM	0.0219 mL	0.1095 mL	0.2190 mL	0.5475 mL