2. Metabolic Enzyme/Protease
  3. Tyrosinase
  4. SBB-Analogue (GL13) Biotin

SBB-Analogue (GL13) Biotin  (Synonyms: GL13; SBB-A-B)

Cat. No.: HY-158742 Pureza: 99.30%
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SBB-Analogue (GL13) Biotin (GL13; SBB-A-B) consists of a Sudan Black B (SBB) (HY-D0213) derivative conjugated with biotin. SBB-Analogue (GL13) Biotin potently detects senescent cells and eliminates the drawback of false-positive staining caused by serum starvation and cell fusion in SA-β-gal assays. SBB-Analogue (GL13) Biotin can be used in flow cytometry, immunofluorescence analysis, and other applications.

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SBB-Analogue (GL13) Biotin

SBB-Analogue (GL13) Biotin Estructura química

No. CAS : 2171036-89-6

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Based on 1 publication(s) in Google Scholar

SBB-Analogue (GL13) Biotin Related Antibodies

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1 Publications Citing Use of MCE SBB-Analogue (GL13) Biotin

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Descripciòn

SBB-Analogue (GL13) Biotin (GL13; SBB-A-B) consists of a Sudan Black B (SBB) (HY-D0213) derivative conjugated with biotin. SBB-Analogue (GL13) Biotin potently detects senescent cells and eliminates the drawback of false-positive staining caused by serum starvation and cell fusion in SA-β-gal assays. SBB-Analogue (GL13) Biotin can be used in flow cytometry, immunofluorescence analysis, and other applications[1].

In Vitro

SBB-Analogue (GL13) Biotin specifically stains and detects lipofuscin-containing senescent cells, including U2OS hCdt1 Tet-ON, human diploid lung fibroblasts, HBEC-Cdc6 Tet-ON, and irradiated Saos2-p21WAF1/Cip1 cells. It exhibits higher sensitivity, lower background, and complete consistency with established senescence markers[1].
SBB-Analogue (GL13) Biotin stains perinuclear structures or cytoplasmic granules in senescent cells; when used with an avidin-peroxidase conjugated detection system, it produces a clear insoluble product, thereby enhancing visualization[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SBB-Analogue (GL13) Biotin Related Antibodies
Peso molecular

698.84

Fòrmula

C39H38N8O3S
No. CAS
Appearance

Solid

Color

Brown to black

SMILES

O=C(N1)N[C@](CS2)([H])[C@@]1([H])[C@@H]2CCCCC(OCC(C)(N3)NC4=CC=C(C5=C4C3=CC=C5)/N=N/C6=CC=C(/N=N/C7=CC=CC=C7)C8=C6C=CC=C8)=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Pureza y Documentación

Purity: 99.30%

SDS (252 KB)

COA (249 KB)

Instrucciones de manejo (2659 KB)
Referencias
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SBB-Analogue (GL13) Biotin Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SBB-Analogue (GL13) Biotin2171036-89-6GL13 SBB-A-BGL 13GL-13Tyrosinaseflow cytometrysenescence markersSaos2-p21WAF1/Cip1 cellsimmunofluorescencesenescent cellslipofuscinproliferation markersU2OS hCdt1 Tet-ONHBEC-Cdc6 Tet-ONhuman diploid lung fibroblastInhibitorinhibitorinhibit

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Nombre del producto:
SBB-Analogue (GL13) Biotin
Cat. No.:
HY-158742
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