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N1-Me-Pseudo+UTP

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By Targets:	Biochemical Assay Reagents (1) DNA/RNA Synthesis (1) Fluorescent Dye (1) mRNA (3) Nucleoside Antimetabolite/Analog (1) SARS-CoV (1)
By Research Areas:	Cardiovascular Disease (1) Infection (1)
Oligonucleotides:	Antigens (1) mRNA (4)

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Biochemical Assay Reagents

Oligonucleotides

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-153079

*eGFP mRNA (N1-Me-Pseudo UTP)*	mRNA	Others
eGFP mRNA (N1-Me-Pseudo UTP) will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence. The substitution with N1-Me-Pseudo UTP (m1Ψ) improves protein expression and reduces innate immune response.

HY-153083

*COVID-19 Spike Protein mRNA(N1-Me-Pseudo UTP)* 1 Publications Verification	mRNA SARS-CoV	Infection
COVID-19 Spike Protein mRNA (N1-Me-Pseudo UTP) is an mRNA encoding the SARS-CoV-2 spike protein with enhanced performance via chemical modification. COVID-19 Spike Protein mRNA (N1-Me-Pseudo UTP) replaces natural uridine (UTP) with N1-Me-Pseudo UTP, which effectively reduces immunogenicity and improves stability and translation efficiency. The 3' UTR of COVID-19 Spike Protein mRNA (N1-Me-Pseudo UTP) optimizes AU-rich elements through HuR anchor sites, exhibiting higher translation efficiency. COVID-19 Spike Protein mRNA is widely used in COVID-19-related scientific research and vaccine development ^[1] .

HY-112582C

N1-Methylpseudouridine-5′-triphosphate trisodium solution (100 mM) 1 Publications Verification 1-Methylpseudouridine-5′-triphosphate trisodium; N1-Me-Pseudo UTP trisodium; N1-Methyl-Pseudo-UTP trisodium	Nucleoside Antimetabolite/Analog DNA/RNA Synthesis	Cardiovascular Disease
N1-Methylpseudouridine-5′-triphosphate (1-Methylpseudouridine-5′-triphosphate) trisodium solution (100 mM) is a nucleobase-modified nucleotide. N1-Methylpseudouridine-5′-triphosphate (1-Methylpseudouridine-5′-triphosphate) trisodium solution (100 mM) is used for synthesizing mRNA with reduced immunogenicity and improved stability ^[1].

HY-153080

*Firefly Luciferase mRNA(N1-Me-Pseudo UTP)*	Fluorescent Dye mRNA	Others
Firefly Luciferase mRNA (N1-Me-Pseudo UTP) is a modified form of firefly luciferase mRNA, which is N1-methylated pseudo uridine (N1-Me-Pseudo UTP). Firefly Luciferase mRNA (N1-Me-Pseudo UTP) is a commonly used reporter molecule in biomedical and mRNA research and its core function is to rapidly and quantitatively monitor the expression and delivery efficiency of mRNA through the emission of light signals ^[1].

HY-171493

*mCherry mRNA(N1-Me-Pseudo UTP)*	Biochemical Assay Reagents	Others
mCherry mRNA(N1-Me-Pseudo UTP) is a reporter mRNA encoding a red fluorescent protein, suitable for gene expression analysis, cell viability assays, and in vivo imaging. mCherry, derived from mushroom coral, is a widely used red fluorescent protein tracer in biotechnology applications.

Cat. No.	Product Name	Type

HY-112582C

N1-Methylpseudouridine-5′-triphosphate trisodium solution (100 mM) 1 Publications Verification 1-Methylpseudouridine-5′-triphosphate trisodium; N1-Me-Pseudo UTP trisodium; N1-Methyl-Pseudo-UTP trisodium	Biochemical Assay Reagents
N1-Methylpseudouridine-5′-triphosphate (1-Methylpseudouridine-5′-triphosphate) trisodium solution (100 mM) is a nucleobase-modified nucleotide. N1-Methylpseudouridine-5′-triphosphate (1-Methylpseudouridine-5′-triphosphate) trisodium solution (100 mM) is used for synthesizing mRNA with reduced immunogenicity and improved stability ^[1].

Cat. No.	Product Name		Classification

HY-153079

*eGFP mRNA (N1-Me-Pseudo UTP)*		mRNA
eGFP mRNA (N1-Me-Pseudo UTP) will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence. The substitution with N1-Me-Pseudo UTP (m1Ψ) improves protein expression and reduces innate immune response.

HY-153083

*COVID-19 Spike Protein mRNA(N1-Me-Pseudo UTP)* 1 Publications Verification		mRNA Antigens
COVID-19 Spike Protein mRNA (N1-Me-Pseudo UTP) is an mRNA encoding the SARS-CoV-2 spike protein with enhanced performance via chemical modification. COVID-19 Spike Protein mRNA (N1-Me-Pseudo UTP) replaces natural uridine (UTP) with N1-Me-Pseudo UTP, which effectively reduces immunogenicity and improves stability and translation efficiency. The 3' UTR of COVID-19 Spike Protein mRNA (N1-Me-Pseudo UTP) optimizes AU-rich elements through HuR anchor sites, exhibiting higher translation efficiency. COVID-19 Spike Protein mRNA is widely used in COVID-19-related scientific research and vaccine development ^[1] .

HY-153080

*Firefly Luciferase mRNA(N1-Me-Pseudo UTP)*		mRNA
Firefly Luciferase mRNA (N1-Me-Pseudo UTP) is a modified form of firefly luciferase mRNA, which is N1-methylated pseudo uridine (N1-Me-Pseudo UTP). Firefly Luciferase mRNA (N1-Me-Pseudo UTP) is a commonly used reporter molecule in biomedical and mRNA research and its core function is to rapidly and quantitatively monitor the expression and delivery efficiency of mRNA through the emission of light signals ^[1].

HY-171493

*mCherry mRNA(N1-Me-Pseudo UTP)*		mRNA
mCherry mRNA(N1-Me-Pseudo UTP) is a reporter mRNA encoding a red fluorescent protein, suitable for gene expression analysis, cell viability assays, and in vivo imaging. mCherry, derived from mushroom coral, is a widely used red fluorescent protein tracer in biotechnology applications.

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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