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pH-sensitive fluorescent indicator

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By Targets:	Fluorescent Dye (5) Environmental Pollutants (1)
By Research Areas:	Cancer (1)

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Fluorescent Dyes

Targets Recommended: Fluorescent Dye

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D0023

Pyranine 2 Publications Verification HPTS; Solvent Green 7	Environmental Pollutants Fluorescent Dye	Others
Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D0077

2',7'-Difluorofluorescein Oregon green 488	Fluorescent Dye	Others
2',7'-Difluorofluorescein (Oregon green 488) is a fluorescein derivative and a pH-sensitive fluorescent probe (pK_a ~4.7). Upon excitation at 488 nm, 2',7'-Difluorofluorescein exhibits pH-sensitive fluorescence intensity through the formation of dianions, while its pH sensitivity decreases under excitation at 450 nm, allowing its use in ratiometric pH analysis. 2',7'-Difluorofluorescein can be used for the quantitative analysis of pH values in the range of 2-7 in submicron aerosol particles. 2',7'-Difluorofluorescein undergoes buffer-mediated and buffer-free excited-state proton transfer between different protonated forms, and its cationic form undergoes rapid excited-state deprotonation. 2',7'-Difluorofluorescein is resistant to photodegradation, maintains stable absorption and fluorescence properties within the physiological pH range, and serves as a fluorescent protein label, a component of Ca ²⁺ indicators, a fluorescent imaging agent, and an anisotropy probe .

HY-D1556

18:1 PE CF 1,2-dioleoyl-sn-glycero-3-pHospHoethanolamine-N-(carboxyfluorescein) ammonium	Fluorescent Dye	Cancer
18:1 PE CF (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium)) is a pH-sensitive fluorescent indicator. 18:1 PE CF exhibits changes in fluorescence intensity in response to pH variations and reflects the electrostatic states of cationic liposomes and lipoplexes. 18:1 PE CF supports real-time visualization of pH distribution and membrane deformation in giant unilamellar vesicles and is suitable for steady-state fluorescence testing in large unilamellar vesicle membranes. 18:1 PE CF is capable of monitoring the surface electrical potential of cationic liposomes and lipoplexes and can effectively label lipid bilayers and nanomicelles. 18:1 PE CF can be used for the research of the investigation of lung cancer (Ex = 495 nm; Em = 520 nm) .

HY-D0023A

Pyranine hydrate HPTS hydrate; Solvent Green 7 hydrate	Fluorescent Dye	Others
Pyranine (HPTS; Solvent Green 7) hydrate is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine hydrate acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine hydrate is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D0116

Pyranine free acid HPTS free acid; Solvent Green 7 free acid	Fluorescent Dye	Others
Pyranine (HPTS; Solvent Green 7) free acid is a pH-sensitive fluorescent indicator. Pyranine free acid acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine free acid is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

Cat. No.	Product Name	Type

HY-D0023

Pyranine 2 Publications Verification HPTS; Solvent Green 7	Fluorescent Dyes
Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D1556

18:1 PE CF 1,2-dioleoyl-sn-glycero-3-pHospHoethanolamine-N-(carboxyfluorescein) ammonium	Fluorescent Dyes
18:1 PE CF (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium)) is a pH-sensitive fluorescent indicator. 18:1 PE CF exhibits changes in fluorescence intensity in response to pH variations and reflects the electrostatic states of cationic liposomes and lipoplexes. 18:1 PE CF supports real-time visualization of pH distribution and membrane deformation in giant unilamellar vesicles and is suitable for steady-state fluorescence testing in large unilamellar vesicle membranes. 18:1 PE CF is capable of monitoring the surface electrical potential of cationic liposomes and lipoplexes and can effectively label lipid bilayers and nanomicelles. 18:1 PE CF can be used for the research of the investigation of lung cancer (Ex = 495 nm; Em = 520 nm) .

18:1 PE CF

1,2-dioleoyl-sn-glycero-3-pHospHoethanolamine-N-(carboxyfluorescein) ammonium

Fluorescent Dyes

18:1 PE CF (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium)) is a pH-sensitive fluorescent indicator. 18:1 PE CF exhibits changes in fluorescence intensity in response to pH variations and reflects the electrostatic states of cationic liposomes and lipoplexes. 18:1 PE CF supports real-time visualization of pH distribution and membrane deformation in giant unilamellar vesicles and is suitable for steady-state fluorescence testing in large unilamellar vesicle membranes. 18:1 PE CF is capable of monitoring the surface electrical potential of cationic liposomes and lipoplexes and can effectively label lipid bilayers and nanomicelles. 18:1 PE CF can be used for the research of the investigation of lung cancer (Ex = 495 nm; Em = 520 nm) .

HY-D0023A

Pyranine hydrate HPTS hydrate; Solvent Green 7 hydrate	Fluorescent Dyes
Pyranine (HPTS; Solvent Green 7) hydrate is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine hydrate acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine hydrate is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

HY-D0116

Pyranine free acid HPTS free acid; Solvent Green 7 free acid	Fluorescent Dyes
Pyranine (HPTS; Solvent Green 7) free acid is a pH-sensitive fluorescent indicator. Pyranine free acid acts as a class of fluorescent chemosensor for the Cu ⁺ ion. Pyranine free acid is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm) .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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