2. GPCR/G Protein
  3. GLP Receptor
  4. tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu  (Synonyms: Semaglutide side chain)

Art. -Nr.: HY-45894 Reinheit: 98.04%
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Handling Instructions Technical Support

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu is an intermediate in the synthesis of the glucagon-like peptide 1 receptor (GLP-1R) agonist Semaglutide (HY-114118). tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu exhibits a strong binding affinity to albumin and can be used to modify SN38 prodrugs to improve tumor distribution.

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Kundenspezifische Peptidsynthese

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu Chemische Struktur

CAS. Nr. : 1118767-16-0

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tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu Related Antibodies

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Beschreibung

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu is an intermediate in the synthesis of the glucagon-like peptide 1 receptor (GLP-1R) agonist Semaglutide (HY-114118). tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu exhibits a strong binding affinity to albumin and can be used to modify SN38 prodrugs to improve tumor distribution[1].

In Vitro

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu (Semaglutide side chain), serves either as a starting material for the solid-phase synthesis of semaglutide or as a direct coupling agent with fermentation-derived peptide intermediates in semaglutide manufacturing. This molecule introduces three critical structural components: the C18 fatty diacid chain, the hydrophilic AEEA-AEEA linker, and a glutamic acid residue.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu Related Antibodies
Molekulargewicht

846.10

Formel

C43H79N3O13
CAS. Nr.
Appearance

Solid

Color

Colorless to off-white

Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 200 mg/mL (236.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1819 mL 5.9095 mL 11.8189 mL
5 mM 0.2364 mL 1.1819 mL 2.3638 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (5.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (5.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1819 mL 5.9095 mL 11.8189 mL 29.5473 mL
5 mM 0.2364 mL 1.1819 mL 2.3638 mL 5.9095 mL
10 mM 0.1182 mL 0.5909 mL 1.1819 mL 2.9547 mL
15 mM 0.0788 mL 0.3940 mL 0.7879 mL 1.9698 mL
20 mM 0.0591 mL 0.2955 mL 0.5909 mL 1.4774 mL
25 mM 0.0473 mL 0.2364 mL 0.4728 mL 1.1819 mL
30 mM 0.0394 mL 0.1970 mL 0.3940 mL 0.9849 mL
40 mM 0.0295 mL 0.1477 mL 0.2955 mL 0.7387 mL
50 mM 0.0236 mL 0.1182 mL 0.2364 mL 0.5909 mL
60 mM 0.0197 mL 0.0985 mL 0.1970 mL 0.4925 mL
80 mM 0.0148 mL 0.0739 mL 0.1477 mL 0.3693 mL
100 mM 0.0118 mL 0.0591 mL 0.1182 mL 0.2955 mL
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tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

tBuO-Ste-Glu(AEEA-AEEA-OH)-OtBu1118767-16-0Semaglutide side chainGLP ReceptorInhibitorinhibitorinhibit

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