α-1-3,4 Fucosyltransferase, helicobacter pylori  (Synonyms: α-1-3,4 FucT; Hp3/4FT)

alpha-1,3/4-Fucosyltransferase (α1,3/4FucT) (EC 2.4.1.65) (Hp3/4FT) can be found in Helicobacter pylori. alpha-1,3/4-Fucosyltransferase (α1,3/4FucT) catalyzes fucose transfer from donor GDP-beta-l-fucose to the GlcNAc^[1].

Product Information
This product is recombinantly expressed in E. coli, with a C-terminal His-tag, a molecular weight of 50 kDa, and a buffer component of 20 mM Tris-HCl, pH 8.0, and 10% glycerol. It is a key enzyme for the synthesis of sLex and Lex.

Instructions
1. Add substrate according to the reaction system, 5 mM MnCl₂, and appropriate buffer salts such as Tris-HCl can be added to adjust the pH to 8.0, and add appropriate amount of α1,3/4-FucT (according to reaction requirements);
2. After mixing, incubate the sample at 37°C for more than 2h or overnight.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37277-69-3

Liquid

Colorless to light yellow

[α-1-3,4 Fucosyltransferase, helicobacter pylori]

2.4.1.65

≥0.1 U/mg soild

One unit is defined as the amount of enzyme that catalyzes the transfer of 1 umo! fucose from GDpfucose to lactose per minute at 37°C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ma B, et al. C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer. J Biol Chem. 2003 Jun 13;278(24):21893-900.  [Content Brief]