11β-HSD1-IN-25

11β-HSD1 lentivirus induced (or not) (i.v.)-male C57BL/6 mice (8 weeks, 20-22 g)^[1]

0.5 mg/kg

p.o., daily for 60 days

Significantly mitigated weight gain when induced by 11β-HSD1 lentivirus.
Significantly reduced serum concentrations of corticosterone (CORT), cholesterol (CHO), triglycerides (TG), low-density lipoprotein (LDL), and high density lipoprotein (HDL).
Improved adipocyte morphology.
Decreased 11β-HSD1 protein expression.
Exhibited a substantial increase in the protein expression levels of UCP1, the p-AMPK/AMPK ratio, and CPT1 when reduced by 11β-HSD1 lentivirus when reduced by 11β-HSD1 lentivirus.
Showed a significant reduction in the mRNA expression of Hsd11b1, Srebp1c, Fas, and Acc1 and exhibited a increase in the expression levels of Cpt1, Ucp1, and Hsl when reduced by 11β-HSD1 lentivirus.
Not exhibited a significant elevation in FAS protein expression in adipose tissue when not reduced by 11β-HSD1 lentivirus.
Resulted in a substantial reduction in FAS protein expression.
Upregulated HSL protein no matter reduced by 11β-HSD1 lentivirus or not.
Significantly reduced F4/80 protein expression in adipose tissue.