2,5-Anhydro-D-mannitol, 97%

Name: 2,5-Anhydro-D-mannitol, 97%
Brand: MedChemExpress
SKU: HY-W039315
Rating: 4.5 (0 reviews)

Cat. No.: HY-W039315 Purity: 99.86%: Data Sheet
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2,5-Anhydro-D-mannitol, 97% is an antimetabolic fructose analogue. 2,5-Anhydro-D-mannitol, 97% depletes intracellular ATP, reduces oxygen consumption, regulates substrate oxidation, increases phosphomonoester levels, decreases inorganic phosphate levels, and promotes the release of intracellular calcium from intracellular calcium stores via phospholipase C-dependent activity in hepatocytes.

For research use only. We do not sell to patients.

2,5-Anhydro-D-mannitol, 97% Chemical Structure

CAS No. : 41107-82-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Description

In Vitro

2,5-Anhydro-D-mannitol, 97% (0-1.2 mM; 30 min) reduces ATP content in isolated HC/LF and HF/LC rat hepatocytes in a dose-dependent manner over 30 min, with a significantly larger reduction in HC/LF cells than HF/LC cells in no-substrate and 1 mM oleate-containing media, and equivalent reduction in 5 mM glucose-containing media^[1].
2,5-Anhydro-D-mannitol, 97% (1 mM; 70-80 min) induces greater declines in ATP and greater increases in PME in isolated HC/LF rat hepatocytes than in HF/LC rat hepatocytes over 70-80 min of superfusion, while reducing Pi content equally in both cell groups, with partial recovery of all compounds after removal of the reagent^[1].
2,5-Anhydro-D-mannitol, 97% (2.4 mM; 30 min (no preincubation); 30 min after 1 h preincubation with substrate) reduces oxygen consumption in isolated HC/LF and HF/LC rat hepatocytes over 30 min, with the magnitude of reduction dependent on the substrate present and preincubation conditions, including a significantly larger reduction in HC/LF cells without preincubation in glucose or oleate, and equivalent reduction in both cell groups after glucose preincubation^[1].
2,5-Anhydro-D-mannitol, 97% (0-4 mM; 1 h) inhibits fructose oxidation in a dose-dependent manner over 1 h, with significantly greater inhibition in isolated HC/LF rat hepatocytes than in HF/LC rat hepatocytes, while increasing palmitate oxidation in HC/LF cells and only minimally inhibiting palmitate oxidation in HF/LC cells at concentrations ≥1 mM^[1].
2,5-Anhydro-D-mannitol, 97% (2,5-AM) (1-5 mM; until peak response reached and until [Ca²⁺]_i returned to baseline after washout) induces a statistically significant increase in intracellular calcium in a subset of isolated rat hepatocytes, with the response originating from intracellular calcium stores and dependent on phospholipase C activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2,5-Anhydro-D-mannitol, 97% (100-800 mg/kg; i.p.; single injection) does not increase food intake in healthy female Syrian hamsters at doses from 100 to 400 mg/kg, and reduces food intake at the 800 mg/kg dose^[2].
2,5-Anhydro-D-mannitol, 97% (400 mg/kg; i.p.; single injection) produces a mild, transient decrease in plasma glucose levels in healthy female Syrian hamsters^[2].
2,5-Anhydro-D-mannitol, 97% (400 mg/kg; i.p.; every 6 h; 48 h) does not disrupt estrous cyclicity in female Syrian hamsters, but reduces food intake on the first day of treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Lak:LVG (female, 120-130 g)^[2]
Dosage:	100 mg/kg; 200 mg/kg; 400 mg/kg; 800 mg/kg
Administration:	i.p.; single injection
Result:	Showed no statistically significant increases in food intake at 100, 200, or 400 mg/kg compared to vehicle. Caused a significant decrease in food intake at 800 mg/kg, with lower intake than the vehicle group at 3, 4, and 8 h post-injection, lower intake than the 100 mg/kg group at 1-8 h post-injection, and lower intake than the 400 mg/kg group at 4 h post-injection.

Animal Model:	Lak:LVG (female, 120-130 g)^[2]
Dosage:	400 mg/kg
Administration:	i.p.; single injection
Result:	Caused a mild but significant decrease in plasma glucose levels (24-30% drop at 1 and 2 h post-injection) compared to vehicle. Showed significantly lower plasma glucose levels at 1, 3, and 5 h post-injection, but not at 7 or 25 h post-injection.

Animal Model:	Lak:LVG (female, 120-130 g, two consecutive 4-day estrous cycles, positive sex behavior test)^[2]
Dosage:	400 mg/kg
Administration:	i.p.; every 6 h; 48 h
Result:	Showed normal 4-day estrous cycles, including robust lordosis behavior and postovulatory vaginal discharge in all treated hamsters. Caused significantly lower 24-h food intake on day 1 of treatment (9.7 g) compared to vehicle controls (14.8 g). Showed no significant effects on daily food intake on days 2, 3, or 4.

Molecular Weight

164.16

Formula

C₆H₁₂O₅

CAS No.

41107-82-8

Appearance

Solid

SMILES

OC[C@@H]1[C@H]([C@@H]([C@H](O1)CO)O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 175 mg/mL (1066.03 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0916 mL	30.4581 mL	60.9162 mL
5 mM	1.2183 mL	6.0916 mL	12.1832 mL
10 mM	0.6092 mL	3.0458 mL	6.0916 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.86%

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Data Sheet (279 KB) SDS (394 KB)

COA (249 KB)

Handling Instructions (2659 KB)

References

[1]. Ji H, et al. Interactions of dietary fat and 2,5-anhydro-D-mannitol on energy metabolism in isolated rat hepatocytes. Am J Physiol Regul Integr Comp Physiol. 2002;282(3):R715-R720.

[2]. Schneider JE, et al. Effects of the fructose analog, 2,5-anhydro-D-mannitol, on food intake and estrous cyclicity in Syrian hamsters. Am J Physiol. 1997;272(3 Pt 2):R935-R939.

[3]. Rawson NE, et al. 2,5-Anhydro-D-mannitol increases hepatocyte calcium: implications for a hepatic hunger stimulus. Biochim Biophys Acta. 2003;1642(1-2):59-66.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	6.0916 mL	30.4581 mL	60.9162 mL	152.2904 mL
	5 mM	1.2183 mL	6.0916 mL	12.1832 mL	30.4581 mL
	10 mM	0.6092 mL	3.0458 mL	6.0916 mL	15.2290 mL
	15 mM	0.4061 mL	2.0305 mL	4.0611 mL	10.1527 mL
	20 mM	0.3046 mL	1.5229 mL	3.0458 mL	7.6145 mL
	25 mM	0.2437 mL	1.2183 mL	2.4366 mL	6.0916 mL
	30 mM	0.2031 mL	1.0153 mL	2.0305 mL	5.0763 mL
	40 mM	0.1523 mL	0.7615 mL	1.5229 mL	3.8073 mL
	50 mM	0.1218 mL	0.6092 mL	1.2183 mL	3.0458 mL
	60 mM	0.1015 mL	0.5076 mL	1.0153 mL	2.5382 mL
	80 mM	0.0761 mL	0.3807 mL	0.7615 mL	1.9036 mL
	100 mM	0.0609 mL	0.3046 mL	0.6092 mL	1.5229 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.