2. Epigenetics
  3. Glycosyltransferase
  4. 2F-Peracetyl-Fucose

2F-Peracetyl-Fucose  (Synonyms: 1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos)

製品番号: HY-W096600 純度: 99.54%
COA 取扱説明書 Technical Support

2F-Peracetyl-Fucose (1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos) acts as a potent fucosyltransferase (FUT) inhibitor.

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CAS 番号 : 188783-78-0

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 165 在庫あり
Solution
10 mM * 1 mL in DMSO USD 165 在庫あり
Solid
25 mg $150 在庫あり
50 mg $230 在庫あり
100 mg $380 在庫あり
250 mg $650 在庫あり
500 mg $850 在庫あり
1 g $1100 在庫あり
5 g $2200 在庫あり
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カスタマーレビュー

Based on 3 publication(s) in Google Scholar

2F-Peracetyl-Fucose 関連抗体

Top Publications Citing Use of Products
RT-PCR
Cell Imaging/Staining
Cell Migration/Invasion Assay
IF
Cell Proliferation/Viability Assay
WB

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519.  [Abstract]

    Inhibition of 2F-Peracetyl-Fucose (2‐FF) (10-50 μM) on colony spheroid growth in HUCCT1‐TRCs and RBE‐TRCs. The representative images of colony spheroids on day 8 (treatment from day3). 2‐FF treatment significantly inhibited the growth of colony spheroids.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519.  [Abstract]

    Effects of 2F-Peracetyl-Fucose (2‐FF) (10-50 μM) on the migration and invasion of HUCCT1‐TRCs and RBE‐TRCs. 2-FF treatment significantly inhibited migration and invasion in ICC-TRCs.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519.  [Abstract]

    Phalloidin staining showed the inhibition of 2F-Peracetyl-Fucose (2‐FF) (50 μM) on F‐actin of HUCCT1‐TRCs and RBE‐TRCs. Red, phalloidin; blue, DAPI. Scale, 20 µm.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519.  [Abstract]

    G‐actin/F‐actin in vivo assay kit and IB was used to investigate the alteration of F‐/G‐actin induced by 2F-Peracetyl-Fucose (2‐FF) (50 μM) treatment (n = 3, t‐test). 2‐FF treatment significantly inhibited F/G‐actin ratio in ICC‐TRCs.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.

    2F-Peracetyl-Fucose (2-FF) (300 μM; 24 h) markedly downregulated the expression levels of FUT2 and FUT8 in DLD-1 cells, indicating that 2-FF effectively suppressed epithelial fucosylation.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.

    Typical images (left) and corresponding statistical results (right) of Transwell invasion and migration assays in DLD-1 cells treated with vehicle, RSV, and 2F-Peracetyl-Fucose (2FF). 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the migration of DLD-1 cells. Scale bar = 50 μm.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.

    Representative images (left) and quantification (right) of wound healing assay at 0 and 24 hours in DLD-1 cells treated with vehicle, RSV, and 2F-Peracetyl-Fucose (2FF). 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the migration of DLD-1 cells. Light blue line marks scratch wound edges. Scale bar = 200 μm. Scratched areas were quantified as a percentage after 24 hours relative to 0 hours.

    2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.

    Measurement of cell proliferation using CCK8 assays. 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the cell proliferation of DLD-1 cells.

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    製品説明

    2F-Peracetyl-Fucose (1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos) acts as a potent fucosyltransferase (FUT) inhibitor[1].

    体外実験

    2F-Peracetyl-Fucose (64 μM, 72 hours) inhibits orosphere formation and invasion in UMSCC14B and UMSCC103 cells[2].
    2F-PAF (25-200 μM, 48 hours) inhibits migration ability in NCI-H3122 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2F-Peracetyl-Fucose 関連抗体

    Cell Invasion Assay[2]

    Cell Line: UMSCC14B, UMSCC103 (head and neck squamous cell carcinoma)
    Concentration: 64 μM
    Incubation Time: 72 hours
    Result: Treated spheres were smaller with reduced invasion.

    Cell Migration Assay [3]

    Cell Line: NCI-H3122 (NSCLC)
    Concentration: 25-200 μM
    Incubation Time: 48 hours
    Result: Reduced number of migrated cells.
    体内実験

    2F-PAF (20 μg/ml, i.v., single dose) inhibits tumor metastasis in Calu-1 xenograft nude mouse model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Calu-1 xenograft BALB/c nude mouse model[3]
    Dosage: 20 μg/ml
    Administration: Intravenous injection (i.v.), single dose
    Result: Reduced tumor colonization ability in the lungs.
    分子量

    292.26

    分子式

    C12H17FO7
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@H]1[C@@H](OC(C)=O)[C@@H](OC(C)=O)[C@H](F)C(OC(C)=O)O1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (342.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (17.11 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4216 mL 17.1081 mL 34.2161 mL
    5 mM 0.6843 mL 3.4216 mL 6.8432 mL
    10 mM 0.3422 mL 1.7108 mL 3.4216 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

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    濃度 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.54%

    COA (251 KB) HNMR (268 KB) RP-HPLC (248 KB) MS (104 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.4216 mL 17.1081 mL 34.2161 mL 85.5403 mL
    5 mM 0.6843 mL 3.4216 mL 6.8432 mL 17.1081 mL
    10 mM 0.3422 mL 1.7108 mL 3.4216 mL 8.5540 mL
    15 mM 0.2281 mL 1.1405 mL 2.2811 mL 5.7027 mL
    DMSO 20 mM 0.1711 mL 0.8554 mL 1.7108 mL 4.2770 mL
    25 mM 0.1369 mL 0.6843 mL 1.3686 mL 3.4216 mL
    30 mM 0.1141 mL 0.5703 mL 1.1405 mL 2.8513 mL
    40 mM 0.0855 mL 0.4277 mL 0.8554 mL 2.1385 mL
    50 mM 0.0684 mL 0.3422 mL 0.6843 mL 1.7108 mL
    60 mM 0.0570 mL 0.2851 mL 0.5703 mL 1.4257 mL
    80 mM 0.0428 mL 0.2139 mL 0.4277 mL 1.0693 mL
    100 mM 0.0342 mL 0.1711 mL 0.3422 mL 0.8554 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    2F-Peracetyl-Fucose Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2F-Peracetyl-Fucose188783-78-01,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranosGlycosyltransferasefucosyltransferaseFUT inhibitorAnti-tumorInhibitorinhibitorinhibit

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    製品名:
    2F-Peracetyl-Fucose
    製品番号:
    HY-W096600
    数量:
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