4μ8C (Synonyms: IRE1 Inhibitor III)

Name: 4μ8C
Brand: MedChemExpress
SKU: HY-19707
Rating: 5.0 (69 reviews)

製品番号: HY-19707 純度: 99.95%: Data Sheet SDS COA 取扱説明書
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4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α.

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CAS 番号 : 14003-96-4

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 60	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 60	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$55	在庫あり	Estimated Time of Arrival: December 31
10 mg	$77	在庫あり	Estimated Time of Arrival: December 31
25 mg	$132	在庫あり	Estimated Time of Arrival: December 31
50 mg	$209	在庫あり	Estimated Time of Arrival: December 31
100 mg	$319	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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Based on 69 publication(s) in Google Scholar

顧客検証

2D/3D Cell Culture and Differentiation

RT-PCR

: 4μ8C purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]; Representative images of mouse colon organoids treated with G'9668 or 4μ8C (10 μM) for 24 h after being differentiated with DAPT for 5 days. Scale bars, 360 μm.

: 4μ8C purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]; Graphs depicting relative mRNA expression of Muc2 in colon organoids treated with G'9668 or 4μ8C (10 μM) for 24 h after incubation, with or without DAPT for 5 days, measured by RT-qPCR.

: 4μ8C purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 22:S1074-7613(25)00332-2. [Abstract]; Graphs depicting relative mRNA expression of Agr2 in colon organoids treated with G'9668 or 4μ8C (10 μM) for 24 h after incubation, with or without DAPT for 5 days, measured by RT-qPCR.

: 4μ8C purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Aug;6(8):1601-1615. [Abstract]; Xbp1 splicing and Gcg expression in NaHS-treated cells in the presence of 60 μM 4μ8c for 2h (n = 4 for all groups).

: 4μ8C purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 Jul;38(7):1756-1767. [Abstract]; 4μ8C (20 μM; 1 h) significantly inhibits NaF-induced increased expression of p-IRE1 , GRP78 , p-TRAF2 , p-JNK, and LC3II/I in LS8 cells.

: 4μ8C purchased from MedChemExpress. Usage Cited in: Toxins. 2020 Jan 16;12(1):55. [Abstract]; The effect of the IRE1 specific inhibitor 4μ8C on the expression of apoptosis-related proteins. The cells are exposed to FB1 with or without 4μ8C for 24 h, and the phosphorylation of JNK, Mcl-1, Bak, Bax, and Puma were analyzed by Western blotting. n = 3.

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製品説明

4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α.

Cellular Effect

Cell Line	Type	Value	Description	References
JeKo-1	IC₅₀	1.57 3 Compound: 5, 4mu8C	Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis	[PMID: 24749861]
JeKo-1	IC₅₀	1.57 3 Compound: 5, 4mu8C	Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis	[PMID: 24749861]
JeKo-1	IC₅₀	1.57 3 Compound: 5, 4mu8C	Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis	[PMID: 24749861]
Sf21	IC₅₀	206 1 Compound: 5, 4mu8C	Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay	[PMID: 24749861]
Sf21	IC₅₀	206 1 Compound: 5, 4mu8C	Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay	[PMID: 24749861]
Sf21	IC₅₀	206 1 Compound: 5, 4mu8C	Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay	[PMID: 24749861]

体外実験

When applies to the media of ER stressed cultured cells, 4μ8C (IRE1 Inhibitor III) inhibits Xbp1 splicing in a concentration-dependent manner. 4μ8C dissociates slowly from IRE1, but ishout of inhibitor leads to rapid recovery of Xbp1 splicing in cells^[1].The IRE1 endoribonuclease inhibitor 4μ8c prevents the splicing of the XBP1 mRNA in response to ER stress caused by mutant proinsulin production^[2]. The inositol-requiring enzyme 1α (IRE1α) is a serine-threonine kinase that plays crucial roles in activating the unfolded protein response. 4μ8C treatment dramatically inhibits IL-4 production by CD4⁺ T cells under Th0 conditions because both the IL-4 levels in the culture supernatant and the percentage of IL-4 positive cells are reduced by 4μ8C treatment. In addition, both IL-5 and IL-13 production are significantly reduced upon treatment with 4μ8C^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

4μ8c (IRE1 Inhibitor III) (i.p. injection; 10 mg/kg/day for 4 more weeks) leads to a significant reduction (45.2%) in atherosclerotic lesion area in en face aorta preparations. 4μ8c can effectively mitigate plaque development in mice^[4].
4μ8C (orally; 10, 50, or 100 mg/kg) suppresses passive cutaneous anaphylaxis (PCA) in mice (ED50 = 25.1 mg/kg)^[5].
4μ8C reverses the ER stress-dependent loss of several known RIDD targets, with an EC₅₀ of approximately 4 μM, approximating that of inhibition of XBP1 target gene activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ApoE^-/- mice^[4]
Dosage:	10 mg/kg
Administration:	I.p. injection; daily; for 4 more weeks
Result:	Led to a significant reduction (45.2%) in atherosclerotic lesion area in en face aorta preparations.

分子量

204.18

分子式

C₁₁H₈O₄

CAS 番号

14003-96-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=CC1=C(O)C=CC2=C1OC(C=C2C)=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 33.33 mg/mL (163.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8976 mL	24.4882 mL	48.9764 mL
5 mM	0.9795 mL	4.8976 mL	9.7953 mL
10 mM	0.4898 mL	2.4488 mL	4.8976 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 25 mg/mL (122.44 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 99.95%

Select Batch:

データシート (276 KB) SDS (393 KB)

COA (250 KB) HNMR (244 KB) RP-HPLC (237 KB) LCMS (95 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Cross BC, et al. The molecular basis for selective inhibition of unconventional mRNA splicing by an IRE1-binding small molecule. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):E869-78. [Content Brief]

[2]. Zhang L, et al. IRE1 inhibition perturbs the unfolded protein response in a pancreatic β-cell line expressing mutant proinsulin, but does not sensitize the cells to apoptosis. BMC Cell Biol. 2014 Jul 10;15:29. [Content Brief]

[3]. Kemp K, et al. The serine-threonine kinase inositol-requiring enzyme 1α (IRE1α) promotes IL-4 production in T helper cells. J Biol Chem. 2013 Nov 15;288(46):33272-82.

[4]. Tufanli O, et al. Targeting IRE1 with small molecules counteracts progression of atherosclerosis. Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):E1395-E1404. [Content Brief]

[5]. Nam ST, et al. Suppression of IgE-mediated mast cell activation and mouse anaphylaxis via inhibition of Sykactivation by 8-formyl-7-hydroxy-4-methylcoumarin, 4μ8C. Toxicol Appl Pharmacol. 2017 Oct 1;332:25-31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8976 mL	24.4882 mL	48.9764 mL	122.4410 mL
	5 mM	0.9795 mL	4.8976 mL	9.7953 mL	24.4882 mL
	10 mM	0.4898 mL	2.4488 mL	4.8976 mL	12.2441 mL
	15 mM	0.3265 mL	1.6325 mL	3.2651 mL	8.1627 mL
	20 mM	0.2449 mL	1.2244 mL	2.4488 mL	6.1220 mL
	25 mM	0.1959 mL	0.9795 mL	1.9591 mL	4.8976 mL
	30 mM	0.1633 mL	0.8163 mL	1.6325 mL	4.0814 mL
	40 mM	0.1224 mL	0.6122 mL	1.2244 mL	3.0610 mL
	50 mM	0.0980 mL	0.4898 mL	0.9795 mL	2.4488 mL
	60 mM	0.0816 mL	0.4081 mL	0.8163 mL	2.0407 mL
	80 mM	0.0612 mL	0.3061 mL	0.6122 mL	1.5305 mL
	100 mM	0.0490 mL	0.2449 mL	0.4898 mL	1.2244 mL

4μ8C Related Classifications

Metabolic Disease

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4μ8C14003-96-4IRE1 Inhibitor IIIIRE1Inositol requiring enzyme 1Inhibitorinhibitorinhibit

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4μ8C (Synonyms: IRE1 Inhibitor III)

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4μ8C 関連抗体

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Complete Stock Solution Preparation Table

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