5-(Biotinamido)pentylamine

Name: 5-(Biotinamido)pentylamine
Brand: MedChemExpress
SKU: HY-W076543
Rating: 4.5 (0 reviews)

Cat. No.: HY-W076543 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

5-(Biotinamido)pentylamine is an amine donor substrate for transglutaminase. 5-(Biotinamido)pentylamine can be used as a biotin-labeled probe to specifically participate in the calcium-dependent reaction catalyzed by TG, bind to the γ-carboxamide group of the glutamine residue in the protein, introduce the biotin tag into the target protein, and form a biotinylated protein product. 5-(Biotinamido)pentylamine can be used for the labeling, separation, and detection of TG amine receptor protein substrates.

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5-(Biotinamido)pentylamine 화학구조

CAS No. : 115416-38-1

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

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TGM2

Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

Alkyl-Chain

In Vitro

5-(Biotinamido)pentylamine (5 mM; 1 h) can specifically label a variety of protein substrates in mouse, rat, and guinea pig liver extracts through the catalysis of endogenous transglutaminase (TG). Glyceraldehyde-3-phosphate dehydrogenase (38 kDa) and glutathione S-transferase (28 kDa) are identified by affinity chromatography and SDS-PAGE separation^[1].
5-(Biotinamido)pentylamine (1 mM; 30 min) can label 26, 40, 55, and 60 kDa proteins in MCF-7 human breast cancer cell lysate under Ca2+-dependent conditions. The 55 kDa protein was significantly labeled in resistant cells, while the labeling was weak in sensitive cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

328.47

화학식

C₁₅H₂₈N₄O₂S

CAS No.

115416-38-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NCCCCCN)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (304.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0444 mL	15.2221 mL	30.4442 mL
5 mM	0.6089 mL	3.0444 mL	6.0888 mL
10 mM	0.3044 mL	1.5222 mL	3.0444 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.96%

Select Batch:

Data Sheet (270 KB) SDS (392 KB)

COA (244 KB) HNMR (294 KB) RP-HPLC (209 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Ikura K, et al. Identification of amine acceptor protein substrates of transglutaminase in liver extracts: use of 5-(biotinamido) pentylamine as a probe. Arch Biochem Biophys. 1998 Aug 15;356(2):280-6. [Content Brief]

[2]. Singh RN, et al. Detection of the amine acceptor protein substrates of transglutaminase with 5-(biotinamido) pentylamine. Anal Biochem. 1995 Oct 10;231(1):261-3. [Content Brief]

[3]. Ichikawa A, et al. Identification of new amine acceptor protein substrate candidates of transglutaminase in rat liver extract: use of 5-(biotinamido) pentylamine as a probe. Biosci Biotechnol Biochem. 2008 Apr;72(4):1056-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0444 mL	15.2221 mL	30.4442 mL	76.1105 mL
	5 mM	0.6089 mL	3.0444 mL	6.0888 mL	15.2221 mL
	10 mM	0.3044 mL	1.5222 mL	3.0444 mL	7.6110 mL
	15 mM	0.2030 mL	1.0148 mL	2.0296 mL	5.0740 mL
	20 mM	0.1522 mL	0.7611 mL	1.5222 mL	3.8055 mL
	25 mM	0.1218 mL	0.6089 mL	1.2178 mL	3.0444 mL
	30 mM	0.1015 mL	0.5074 mL	1.0148 mL	2.5370 mL
	40 mM	0.0761 mL	0.3806 mL	0.7611 mL	1.9028 mL
	50 mM	0.0609 mL	0.3044 mL	0.6089 mL	1.5222 mL
	60 mM	0.0507 mL	0.2537 mL	0.5074 mL	1.2685 mL
	80 mM	0.0381 mL	0.1903 mL	0.3806 mL	0.9514 mL
	100 mM	0.0304 mL	0.1522 mL	0.3044 mL	0.7611 mL