2. Epigenetics
  3. Epigenetic Reader Domain
  4. 5-Ph-IAA

5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression.

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CAS 番号 : 168649-23-8

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 在庫あり
Solution
10 mM * 1 mL in DMSO USD 77 在庫あり
Solid
5 mg $70 在庫あり
10 mg $120 在庫あり
25 mg $240 在庫あり
50 mg $400 在庫あり
100 mg $680 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 52 publication(s) in Google Scholar

5-Ph-IAA 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
Cell Imaging/Staining

    5-Ph-IAA purchased from MedChemExpress. Usage Cited in: Nature. 2023 Jun;618(7963):180-187.  [Abstract]

    Representative immunoblot in Smarce1-AID clones #6, #23, and #13het treated with 1 μM 5-Ph-IAA (0,10,30 min, 1,2,4, 6 h) for the indicated time following release from mitotic arrest. GAPDH was used as a loading control

    5-Ph-IAA purchased from MedChemExpress. Usage Cited in: Nature. 2023 Jun;618(7963):180-187.  [Abstract]

    Representative immunoblot in mitotic Smarce1-AID cells (clones #6 and #23) treated with DMSO or 1 μM 5-Ph-IAA for 1 hour (lanes 1 and 2) and in cells that underwent washout and release from mitotic arrest for the indicated time periods.

    5-Ph-IAA purchased from MedChemExpress. Usage Cited in: Nature. 2023 Jun;618(7963):180-187.  [Abstract]

    Cell morphologies of DMSO treated Smarce1-AID cells (clones #6 and #23) and the cells treated with 1 μM 5-Ph-IAA for the indicated days (0, 1, 3, 7 days).

    5-Ph-IAA purchased from MedChemExpress. Usage Cited in: Nature. 2023 Jun;618(7963):180-187.  [Abstract]

    Representative immunoblot of Smarce1-AID cells (clones #6 and #23) treated with DMSO or 1 μM (30, 60, 240, 480 min, 1, 3, 7 days) 5-Ph-IAA.

    5-Ph-IAA purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Jan 19;83(2):252-265.e13.  [Abstract]

    5-Ph-IAA (5 μM; 1 h) rapidly induces Zpr1 depletion (Fig A), leading to cell growth arrest (Fig B), in ZPR1-AID cells.

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    製品説明

    5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression[1].

    体外実験

    5-Ph-IAA (1 μM; 6 hours) leads to DHC1-mAC degradation in HCT116 cells constitutively expressing OsTIR1(F74G), which means the AID2 system better than the original AID system in generating conditional mutant cell lines[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    5-Ph-IAA 関連抗体

    Western Blot Analysis[1]

    Cell Line: HCT116 cells
    Concentration: 1 µM
    Incubation Time: 6 hours
    Result: Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G).
    体内実験

    5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system, is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells[1]
    Dosage: 0 mg/kg, 1 mg/kg, 3 mg/kg and 10 mg/kg
    Administration: Intraperitoneally injection; every day; 7 days
    Result: Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts.
    分子量

    251.28

    分子式

    C16H13NO2
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(O)CC1=CNC2=C1C=C(C3=CC=CC=C3)C=C2
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (397.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9796 mL 19.8981 mL 39.7962 mL
    5 mM 0.7959 mL 3.9796 mL 7.9592 mL
    10 mM 0.3980 mL 1.9898 mL 3.9796 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 1 mg/mL (3.98 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.80%

    COA (250 KB) HNMR (164 KB) RP-HPLC (267 KB) MS (71 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.9796 mL 19.8981 mL 39.7962 mL 99.4906 mL
    5 mM 0.7959 mL 3.9796 mL 7.9592 mL 19.8981 mL
    10 mM 0.3980 mL 1.9898 mL 3.9796 mL 9.9491 mL
    15 mM 0.2653 mL 1.3265 mL 2.6531 mL 6.6327 mL
    20 mM 0.1990 mL 0.9949 mL 1.9898 mL 4.9745 mL
    25 mM 0.1592 mL 0.7959 mL 1.5918 mL 3.9796 mL
    30 mM 0.1327 mL 0.6633 mL 1.3265 mL 3.3164 mL
    40 mM 0.0995 mL 0.4975 mL 0.9949 mL 2.4873 mL
    50 mM 0.0796 mL 0.3980 mL 0.7959 mL 1.9898 mL
    60 mM 0.0663 mL 0.3316 mL 0.6633 mL 1.6582 mL
    80 mM 0.0497 mL 0.2487 mL 0.4975 mL 1.2436 mL
    100 mM 0.0398 mL 0.1990 mL 0.3980 mL 0.9949 mL
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    5-Ph-IAA Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5-Ph-IAA168649-23-8Epigenetic Reader DomainAID2auxinOsTIR1antitumorInhibitorinhibitorinhibit

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    製品名:
    5-Ph-IAA
    製品番号:
    HY-134653
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