1. Cell Cycle/DNA Damage
  2. DNA Stain
  3. 5(6)-ROX

5(6)-ROX  (Synonyms: 5(6)-Carboxy-X-rhodamine)

Cat. No.: HY-D0043 Pureza: 98.50%
Instrucciones de manejo Technical Support

5(6)-ROX (5(6)-Carboxy-X-rhodamine) is a nucleic acid fluorescent label which can be used as a reference dye for real-time polymerase chain reaction (Em/Ex = 605/585 nm).

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5(6)-ROX

5(6)-ROX Estructura química

No. CAS : 198978-94-8

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
5 mg En stock
10 mg En stock
50 mg   Obtener un presupuesto  
100 mg   Obtener un presupuesto  

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This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 5(6)-ROX

  • Actividad biológica

  • Pureza y Documentación

  • Referencias

  • Revisión del cliente

Descripciòn

5(6)-ROX (5(6)-Carboxy-X-rhodamine) is a nucleic acid fluorescent label which can be used as a reference dye for real-time polymerase chain reaction (Em/Ex = 605/585 nm)[1].

In Vitro

5(6)-ROX is the mixture of 5-ROX (HY-D0784) and 6-ROX (HY-D0053).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

534.60

Fòrmula

C33H30N2O5

No. CAS
Appearance

Solid

Color

Brown to breen

Emission (Em)

605

Excitation (Ex)

585

SMILES

[O-]C(C1=CC(C(O)=O)=CC=C1C(C2=C(O3)C4=C5N(CCC4)CCCC5=C2)=C6C3=C(CCC7)C8=[N+]7CCCC8=C6)=O.[O-]C(C9=CC=C(C(O)=O)C=C9C(C%10=C(O%11)C%12=C%13N(CCC%12)CCCC%13=C%10)=C%14C%11=C(CCC%15)C%16=[N+]%15CCCC%16=C%14)=O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad
In Vitro: 

DMSO : 20 mg/mL (37.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8706 mL 9.3528 mL 18.7056 mL
5 mM 0.3741 mL 1.8706 mL 3.7411 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 98.50%

Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8706 mL 9.3528 mL 18.7056 mL 46.7639 mL
5 mM 0.3741 mL 1.8706 mL 3.7411 mL 9.3528 mL
10 mM 0.1871 mL 0.9353 mL 1.8706 mL 4.6764 mL
15 mM 0.1247 mL 0.6235 mL 1.2470 mL 3.1176 mL
20 mM 0.0935 mL 0.4676 mL 0.9353 mL 2.3382 mL
25 mM 0.0748 mL 0.3741 mL 0.7482 mL 1.8706 mL
30 mM 0.0624 mL 0.3118 mL 0.6235 mL 1.5588 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
5(6)-ROX
Cat. No.:
HY-D0043
Cantidad:
MCE Japan Authorized Agent: