6-ROX
Based on 1 Customer Validation
6-ROX is a selective fluorescent probe and potential inhibitor of COX-2. 6-ROX binds to the active site of COX-2 and inhibits its conversion of arachidonic acid into prostaglandins. 6-ROX is often used in the field of optical imaging related to tumors and inflammation, and helps detect diseased tissues with high expression of COX-2.
For research use only. We do not sell to patients.
- Purity: 98.43%
- CAS No.: 194785-18-7
- Formula: C33H30N2O5
- Molecular Weight:534.60
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
6-ROX can be conjugated to COX1/2 inhibitors such as Indomethacin (HY-14397). The 6-ROX conjugate can effectively cross the cell membrane to inhibit COX-2, and exhibits COX-2 inhibitory activity in LPS-activated RAW264.7 cells and 1483 HNSCC cells[1].
In in vitro cell optical imaging experiments (200 nM; 30 min treatment, 30 min washout)，the 6-ROX conjugate can induce strong red fluorescence in 1483 HNSCC cells that highly express COX-2, and this uptake and labeling can be blocked by the COX inhibitor Indomethacin (5 μM, 10 μM; 20 min) [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
585
568
Chemical Information
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CAS No. 194785-18-7
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Appearance Solid
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Molecular Weight 534.60
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Formula C33H30N2O5
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Color Brown to black
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SMILES
O=C(C1=CC=C(C(O)=O)C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)=C1)[O-]
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Synonyms
6-Carboxy-X-rhodamine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (46.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Uddin MJ, et al. Design, synthesis, and structure-activity relationship studies of fluorescent inhibitors of cycloxygenase-2 as targeted optical imaging agents. Bioconjug Chem. 2013 Apr 17;24 (4) :712-23. [Content Brief]
[2]. Butkevich AN, et al. Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED. Chemistry. 2017 Sep 7;23 (50) :12114-12119. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8706 mL | 9.3528 mL | 18.7056 mL | 46.7639 mL |
| 5 mM | 0.3741 mL | 1.8706 mL | 3.7411 mL | 9.3528 mL | |
| 10 mM | 0.1871 mL | 0.9353 mL | 1.8706 mL | 4.6764 mL | |
| 15 mM | 0.1247 mL | 0.6235 mL | 1.2470 mL | 3.1176 mL | |
| 20 mM | 0.0935 mL | 0.4676 mL | 0.9353 mL | 2.3382 mL | |
| 25 mM | 0.0748 mL | 0.3741 mL | 0.7482 mL | 1.8706 mL | |
| 30 mM | 0.0624 mL | 0.3118 mL | 0.6235 mL | 1.5588 mL | |
| 40 mM | 0.0468 mL | 0.2338 mL | 0.4676 mL | 1.1691 mL |
6-ROX Related Classifications
HY-D0053 Related Classifications
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