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  4. 6-ROX

6-ROX  (Synonyms: 6-Carboxy-X-rhodamine)

Cat. No.: HY-D0053 Purity: 98.43%
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6-ROX is a selective fluorescent probe and potential inhibitor of COX-2. 6-ROX binds to the active site of COX-2 and inhibits its conversion of arachidonic acid into prostaglandins. 6-ROX is often used in the field of optical imaging related to tumors and inflammation, and helps detect diseased tissues with high expression of COX-2.

For research use only. We do not sell to patients.

6-ROX

6-ROX Chemical Structure

CAS No. : 194785-18-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
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6-ROX Related Antibodies

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Description

6-ROX is a selective fluorescent probe and potential inhibitor of COX-2. 6-ROX binds to the active site of COX-2 and inhibits its conversion of arachidonic acid into prostaglandins. 6-ROX is often used in the field of optical imaging related to tumors and inflammation, and helps detect diseased tissues with high expression of COX-2[1][2].

In Vitro

6-ROX can be conjugated to COX1/2 inhibitors such as Indomethacin (HY-14397). The 6-ROX conjugate can effectively cross the cell membrane to inhibit COX-2, and exhibits COX-2 inhibitory activity in LPS-activated RAW264.7 cells and 1483 HNSCC cells[1].
In in vitro cell optical imaging experiments (200 nM; 30 min treatment, 30 min washout)，the 6-ROX conjugate can induce strong red fluorescence in 1483 HNSCC cells that highly express COX-2, and this uptake and labeling can be blocked by the COX inhibitor Indomethacin (5 μM, 10 μM; 20 min) [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6-ROX Related Antibodies
Molecular Weight

534.60

Formula

C33H30N2O5
CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

585
Excitation (Ex)

568
SMILES

O=C(C1=CC=C(C(O)=O)C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)=C1)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (46.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8706 mL 9.3528 mL 18.7056 mL
5 mM 0.3741 mL 1.8706 mL 3.7411 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8706 mL 9.3528 mL 18.7056 mL 46.7639 mL
5 mM 0.3741 mL 1.8706 mL 3.7411 mL 9.3528 mL
10 mM 0.1871 mL 0.9353 mL 1.8706 mL 4.6764 mL
15 mM 0.1247 mL 0.6235 mL 1.2470 mL 3.1176 mL
20 mM 0.0935 mL 0.4676 mL 0.9353 mL 2.3382 mL
25 mM 0.0748 mL 0.3741 mL 0.7482 mL 1.8706 mL
30 mM 0.0624 mL 0.3118 mL 0.6235 mL 1.5588 mL
40 mM 0.0468 mL 0.2338 mL 0.4676 mL 1.1691 mL
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6-ROX Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-ROX194785-18-76-Carboxy-X-rhodamineFluorescent DyeInhibitorinhibitorinhibit

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