A-769662
Based on 47 publication(s) in Google Scholar
A-769662 is a AMP-activated protein kinase (AMPK) activator. A-769662 inhibits the function of the 26S proteasome by an AMPK-independent mechanism and leads to cell cycle arrest. A-769662 directly stimulates partially purified rat liver AMPK (EC50 = 0.8 μM) and inhibits fatty acid synthesis in primary rat hepatocytes (IC50 = 3.2 μM). A-769662 can alleviate the symptoms of metabolic diseases such as type 2 diabetes.
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- Pureza: 99.33%
- No. CAS: 844499-71-4
- Fòrmula: C20H12N2O3S
- Peso molecular:360.39
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Publications Citing Use of MedChemExpress (MCE) A-769662
More- Nat Nanotechnol. 2021 Jul;16(7):830-839. [Abstract]
- Cell Metab. 2019 Jul 2;30(1):157-173.e7. [Abstract]
- Immunity. 2024 Jun 11;57(6):1289-1305.e9. [Abstract]
- Cancer Commun (Lond). 2025 Mar;45(3):245-280. [Abstract]
- Nat Cell Biol. 2026 Jun;28(6):1204-1218. [Abstract]
- Nat Commun. 2025 Feb 18;16(1):1736. [Abstract]
- Nat Commun. 2025 Feb 4;16(1):1343. [Abstract]
- Nat Commun. 2023 Dec 14;14(1):8316. [Abstract]
- Nat Commun. 2019 Feb 6;10(1):620. [Abstract]
- Metabolism. 2026 May 20:182:156651. [Abstract]
- Mol Cell. 2017 Oct 19;68(2):336-349.e6. [Abstract]
- J Nanobiotechnology. 2026 Jun 14. [Abstract]
- J Biomed Sci. 2023 Nov 7;30(1):91. [Abstract]
- Cell Commun Signal. 2023 May 4;21(1):99. [Abstract]
- Cell Commun Signal. 2022 Jul 16;20(1):105. [Abstract]
- J Neuroinflammation. 2023 Jun 1;20(1):137. [Abstract]
- Phytomedicine. 2026 Jan:150:157688. [Abstract]
- Phytomedicine. 2025 May 19:145:156776. [Abstract]
- Acta Pharmacol Sin. 2025 Mar 25. [Abstract]
- Cell Chem Biol. 2026 Jun 18;33(6):767-784.e7. [Abstract]
- Int J Biol Macromol. 2025 May 8;311(Pt 4):144076. [Abstract]
- EMBO J. 2024 Dec;43(24):6383-6409. [Abstract]
- EMBO Mol Med. 2023 Sep 11;15(9):e17399. [Abstract]
- Cell Rep. 2025 Jun 13;44(6):115851. [Abstract]
- Cell Rep. 2022 Nov 22;41(8):111707. [Abstract]
- Front Immunol. 2018 Dec 18:9:3026. [Abstract]
- Food Biosci. 2026 Jun 8;81:109140.
- J Diabetes. 2024 Jul;16(7):e13516. [Abstract]
- Int Immunopharmacol. 2025 Jul 28:160:114985. [Abstract]
- Int Immunopharmacol. 2023 Jul:120:110410. [Abstract]
- Mol Neurobiol. 2017 Apr;54(3):2301-2312. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Jan;1871(1):167510. [Abstract]
- Sci Rep. 2021 Jun 11;11(1):12387. [Abstract]
- J Cell Mol Med. 2021 Aug;25(16):7642-7659. [Abstract]
- Viruses. 2019 Dec 10;11(12):1145. [Abstract]
- Mol Immunol. 2023 Aug:160:44-54. [Abstract]
- Mol Cell Endocrinol. 2022 May 1:547:111598. [Abstract]
- Andrology. 2020 May;8(3):663-670. [Abstract]
- J Appl Toxicol. 2026 Feb 2. [Abstract]
- PLoS One. 2024 Mar 7;19(3):e0296792. [Abstract]
- Reprod Sci. 2021 Aug;28(8):2314-2322. [Abstract]
- Immunol Res. 2025 Feb 11;73(1):49. [Abstract]
- Biochem Biophys Res Commun. 2024 Oct 20:730:150367. [Abstract]
- Virus Genes. 2022 Apr;58(2):133-142. [Abstract]
- bioRxiv. 2024 Oct 29:2024.10.25.620328. [Abstract]
- Research Square Preprint. 2021 Dec.
- Patent. US20180263995A1.
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Ver todos los productos específicos de isoformas AMPK
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Actividad biológica
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AMPK 0.8 μM (EC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Hepatocyte | IC50 |
3.2 μM
Compound: 2, A-769662
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Activation of AMPK in rat primary hepatocytes assessed as inhibition of fatty acid biosynthesis by 14C-acetate incorporation assay
Activation of AMPK in rat primary hepatocytes assessed as inhibition of fatty acid biosynthesis by 14C-acetate incorporation assay
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[PMID: 24900695] |
A-769662 (0.1-100 μM) increases AMPK activity in the presence of a saturating concentration of AMP (300 μM)[1].
A-769662 (1 nM-1 mM, 4h) increases acetyl-CoA carboxylase (ACC) phosphorylation dose-dependently in primary rat hepatocytes[1].
A-769662 (50-300 μM, 1 h) increases the GFP-dependent fluorescence dose-dependently in HEK-GFPu-1 cells[3].
A-769662 (0-50 μM, 1 h) is able to reduce the activity of pre-existing proteasomal activity in MEF cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary rat hepatocytes
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Concentration:Primary rat hepatocytes
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Incubation Time:4 h
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Result:Increased ACC phosphorylation dose-dependently.
Showed that increased ACC phosphorylation correlated with inhibition of fatty acid synthesis as measured by incorporation of 14C-acetate into fatty acids with IC50 of 3.2 μM.
A-769662 (3-30 mg/kg, i.p., b.i.d., for 5 d) reduces plasma glucose levels, lipids, and ACC activity in ob/ob and lean littermate mice[1].
A-769662 (3-30 mg/kg, i.p., b.i.d., for 14 d) reduces plasma glucose in ob/ob and lean littermate mice[1].
A-769662 (10-300 μM, 1 h) inhibits the proteasomal function in mouse embryonic fibroblast (MEF) cells with an IC50 of 62 μM in wild-type and double α1 -/- α1 -/- KO mice [3].
A-769662 (300 μM) has toxic effects on MEF cells in wild-type and double α1 -/- α1 -/- KO mice[3].
A-769662 (15-30 mg/kg, i.p., 2 w) induces AMPK activation that normalizes the nerve functional changes and reduces pain associated with diabetic neuropathy (DN) in diabetic mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague Dawley rats fed ad libitum prior were first acclimated by placing them in a gas exchange indirect calorimeter without food for 2 h then gavaged with a mixed meal plus A-769662[1]
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Dosage:30 mg/kg
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Administration:Intraperitoneal injection (i.p.), single dose
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Result:Resulted in 33% reduction of malonyl CoA levels in livers of treated animals in relative to the vehicle.
Had a statistically nonsignificant decrease in malonyl CoA levels in skeletal muscle of rats treated with A-769662.
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Animal Model:6- to 7-weeks-old ob/ob and lean littermate mice[3]
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Dosage:3 or 30 mg/kg b.i.d. for 5 days; 3, 10, or 30 mg/kg b.i.d. for 14 days
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Administration:Intraperitoneal injection (i.p.)
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Result:Significantly decreases fed plasma glucose (30-40% reduction) at 30 mg/kg in both 5 d and 14 d studies.
Reduced 42% of the fed glucose levels (16 h postdose) in the 5 day study.
Resulted in a modest reduction in body weight gain in ob/ob mice treated with 30 mg/kg dose for 5 days.
Resulted in a transient reduction in food intake in the 14 d study.
Increased the compound concentrations in liver 1 h after 30 or 3 mg/kg of A-769662 approximately 30-fold or 2.5-fold above the cellular IC50 for fatty acid synthesis.
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Animal Model:Healthy male Sprague-Dawley rats (250-270 g) fed on standard diet and water ad libitum then induced diabetes with Streptozotocin (HY-13753) (55 mg/kg, i.p.)[4]
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Dosage:15 or 30 mg/kg for 14 days
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Administration:Intraperitoneal injection (i.p.)
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Result:Attenuated the pain hypersensitivity and resulted in improved tail flick latencies and paw withdrawal threshold.
Resulted in marked reduction in motor/sensory nerve conduction velocities (MNCV and SNCV) as well as restoring impaired nerve blood flow (NBF).
Significantly reduced TUNEL-positive cells, inhibited the lipid peroxidation and improved the antioxidant defence by improving glutathione levels in the sciatic nerves of treated diabetes rats.
Significantly reduces the expression of IL-6 and TNF-α and reduced the expression of NF-κB and COX-2 compared to diabetic rats.
Reversed AMPK inactivation and associated mitochondrial biogenesis and autophagy deficits in diabetic rats.
Significantly elevated mitochondrial ATP production in treated rats.
Chemical Information
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No. CAS 844499-71-4
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Appearance Solid
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Peso molecular 360.39
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Fòrmula C20H12N2O3S
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Color Off-white to yellow
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SMILES
N#CC1C(C2=C(NC1=O)SC=C2C3=CC=C(C4=CC=CC=C4O)C=C3)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (47)
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Journal Impact Factor
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Most Recent
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Nat Nanotechnol
Therapeutically reprogrammed nutrient signalling enhances nanoparticulate albumin bound drug uptake and efficacy in KRAS-mutant cancer. [Abstract]2021 Jul;16(7):830-839. PMID: 33958764 -
Cell Metab
2019 Jul 2;30(1):157-173.e7. PMID: 31155494 -
Immunity
Metabolic regulator LKB1 controls adipose tissue ILC2 PD-1 expression and mitochondrial homeostasis to prevent insulin resistance. [Abstract]2024 Jun 11;57(6):1289-1305.e9. PMID: 38772366
A-769662 purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jun 11;57(6):1289-1305.e9. [Abstract]
Immunoblots of P-AMPK and total AMPK in VAT ILC2s.VAT ILC2s sorted fromId2 cre-ERT2 andId2 cre-ERT2Stk11f/f mice 5 days post tamoxifen administration were treated with A-769662 (2μM) in vitro for 5 days.
A-769662 purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jun 11;57(6):1289-1305.e9. [Abstract]
Frequency of IL-5+ ILC2s (left), IL-13+ILC2s (right).VAT ILC2s sorted fromId2 cre-ERT2 andId2 cre-ERT2Stk11 f/f mice 5 days post tamoxifen administration were treated with A-769662 (2μM) in vitro for 5 days.
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Cancer Commun (Lond)
Orchestrated desaturation reprogramming from stearoyl-CoA desaturase to fatty acid desaturase 2 in cancer epithelial-mesenchymal transition and metastasis. [Abstract]2025 Mar;45(3):245-280. PMID: 39722173
A-769662 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Mar;45(3):245-280. [Abstract]
Western blotting of CDH2, phosphorylated AMPKαThr172, and phosphorylated S6Ser235/236 in SW780 and MCF7 treated 48 h with A939572 (10 µmol/L), A-769662 (A769662;100 µmol/L), and Rapamycin (20 µmol/L).
A-769662 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Mar;45(3):245-280. [Abstract]
RT-qPCR measurement of CDH2, CDH1, SNAI2, and FABP4 in SW780 and MCF7 after A-769662（A769662） treatment (100 µmol/L； 48 h).
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Nat Cell Biol
2026 Jun;28(6):1204-1218. PMID: 42174134 -
Nat Commun
A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma. [Abstract]2025 Feb 18;16(1):1736. PMID: 39966385 -
Nat Commun
The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signaling. [Abstract]2025 Feb 4;16(1):1343. PMID: 39905041 -
Nat Commun
2023 Dec 14;14(1):8316. PMID: 38097578 -
Nat Commun
Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy. [Abstract]2019 Feb 6;10(1):620. PMID: 30728358 -
Metabolism
2026 May 20:182:156651. PMID: 42162901 -
Mol Cell
2017 Oct 19;68(2):336-349.e6. PMID: 29053957
A-769662 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2017 Oct 19;68(2):336-349.e6. [Abstract]
AMPK DKO SV40-immortalized MEFs are electroporated with plasmids encoding Myc-tagged AMPK α1, Myc-tagged AMPK α2 and Myc-tagged AMPK α2 S > A. 48 hr later, MEFs are starved for 3 hr and treated for 2 hr with DMSO or 50 mM A769662 before protein extraction. Western blot analysis shows the A769662-induced ACC phosphorylation in transfected cells.
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J Nanobiotechnology
2026 Jun 14. PMID: 42289699 -
J Biomed Sci
Spatiotemporal roles of AMPK in PARP-1- and autophagy-dependent retinal pigment epithelial cell death caused by UVA. [Abstract]2023 Nov 7;30(1):91. PMID: 37936170 -
Cell Commun Signal
Promoting AMPK/SR-A1-mediated clearance of HMGB1 attenuates chemotherapy-induced peripheral neuropathy. [Abstract]2023 May 4;21(1):99. PMID: 37143083 -
Cell Commun Signal
Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth. [Abstract]2022 Jul 16;20(1):105. PMID: 35842652 -
J Neuroinflammation
Microglia-derived TNF-α contributes to RVLM neuronal mitochondrial dysfunction via blocking the AMPK-Sirt3 pathway in stress-induced hypertension. [Abstract]2023 Jun 1;20(1):137. PMID: 37264405 -
Phytomedicine
Periplocin and bufalin induce cardiotoxicity by regulating AMPK/SIRT1/PGC-1α pathway to inhibit energy metabolism and trigger autophagy. [Abstract]2026 Jan:150:157688. PMID: 41453294 -
Phytomedicine
Keluoxin reduces renal lipid deposition in diabetic nephropathy via AMPK/NF-κB-mediated mtabolic regulation. [Abstract]2025 May 19:145:156776. PMID: 40578033 -
Acta Pharmacol Sin
Hepatocyte cellular repressor of E1A-stimulated genes 1 protects against acetaminophen-induced liver injury by promoting autophagy. [Abstract]2025 Mar 25. PMID: 40133627 -
Cell Chem Biol
The cytotoxic effect of prexasertib is a consequence of dual inhibition on both CHK1 and AMPK. [Abstract]2026 Jun 18;33(6):767-784.e7. PMID: 42061410 -
Int J Biol Macromol
CD36 molecule and AMP-activated protein kinase signaling drive docosahexaenoic acid-induced lipid remodeling in goat mammary epithelial cells. [Abstract]2025 May 8;311(Pt 4):144076. PMID: 40348225 -
EMBO J
Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes. [Abstract]2024 Dec;43(24):6383-6409. PMID: 39496977 -
EMBO Mol Med
Metabolic switch from fatty acid oxidation to glycolysis in knock-in mouse model of Barth syndrome. [Abstract]2023 Sep 11;15(9):e17399. PMID: 37533404 -
Cell Rep
ENPP1 governs the metabolic regulation of effector T cells in autoimmunity by detecting cytosolic mitochondrial DNA. [Abstract]2025 Jun 13;44(6):115851. PMID: 40516055 -
Cell Rep
circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells. [Abstract]2022 Nov 22;41(8):111707. PMID: 36417875 -
Front Immunol
Inhibiting Glycolysis and ATP Production Attenuates IL-33-Mediated Mast Cell Function and Peritonitis. [Abstract]2018 Dec 18:9:3026. PMID: 30619366 -
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J Diabetes
Duodenal-jejunal bypass surgery activates eNOS and enhances antioxidant system by activating AMPK pathway to improve heart oxidative stress in diabetic cardiomyopathy rats. [Abstract]2024 Jul;16(7):e13516. PMID: 38087869 -
Int Immunopharmacol
OTUD1 inhibits macrophage ferroptosis via regulation of AMPK and GSK3β/β-catenin signaling pathways exerting protective effects in sepsis-induced acute lung injury. [Abstract]2025 Jul 28:160:114985. PMID: 40460602 -
Int Immunopharmacol
Sialic acids promote macrophage M1 polarization and atherosclerosis by upregulating ROS and autophagy blockage. [Abstract]2023 Jul:120:110410. PMID: 37270929 -
Mol Neurobiol
Adenosine Monophosphate-Activated Protein Kinase Abates Hyperglycaemia-Induced Neuronal Injury in Experimental Models of Diabetic Neuropathy: Effects on Mitochondrial Biogenesis, Autophagy and Neuroinflammation. [Abstract]2017 Apr;54(3):2301-2312. PMID: 26957299
A-769662 purchased from MedChemExpress. Usage Cited in: Mol Neurobiol. 2017 Apr;54(3):2301-2312. [Abstract]
Effect of A769662 on high glucose-induced inflammatory changes in N2A cells and inflammatory marker expression in sciatic nerves of STZ induced rats. Representative immunoblot images of iNOS, COX-2, NF-κB, β-actin and corresponding graphical representations of densitometric analysis.
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Biochim Biophys Acta Mol Basis Dis
Pharmacological targeting of AMPK to restore glucose and fatty acid metabolism homeostasis attenuates transplanted kidney fibrosis. [Abstract]2025 Jan;1871(1):167510. PMID: 39278511 -
Sci Rep
AMPK activates Parkin independent autophagy and improves post sepsis immune defense against secondary bacterial lung infections. [Abstract]2021 Jun 11;11(1):12387. PMID: 34117280 -
J Cell Mol Med
Dapagliflozin alleviates cardiac fibrosis through suppressing EndMT and fibroblast activation via AMPKα/TGF-β/Smad signalling in type 2 diabetic rats. [Abstract]2021 Aug;25(16):7642-7659. PMID: 34169635 -
Viruses
Fatty Acids Regulate Porcine Reproductive and Respiratory Syndrome Virus Infection via the AMPK-ACC1 Signaling Pathway. [Abstract]2019 Dec 10;11(12):1145. PMID: 31835577 -
Mol Immunol
Autotaxin promotes the degradation of the mucus layer by inhibiting autophagy in mouse colitis. [Abstract]2023 Aug:160:44-54. PMID: 37356325 -
Mol Cell Endocrinol
Stanniocalcin2 inhibits the epithelial-mesenchymal transition and invasion of trophoblasts via activation of autophagy under high-glucose conditions. [Abstract]2022 May 1:547:111598. PMID: 35157929 -
Andrology
In vitro exposure to metformin activates human spermatozoa at therapeutically relevant concentrations. [Abstract]2020 May;8(3):663-670. PMID: 31944615 -
J Appl Toxicol
Capacitation-Induced Changes in Sperm Motility, AMPK Phosphorylation, and Tyrosine Phosphorylation Are Diminished by Therapeutically Relevant Concentrations of Metformin. [Abstract]2026 Feb 2. PMID: 41628886 -
PLoS One
Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway. [Abstract]2024 Mar 7;19(3):e0296792. PMID: 38452099 -
Reprod Sci
Modulation of Autophagy Through Regulation of 5'-AMP-Activated Protein Kinase Affects Mitophagy and Mitochondrial Function in Primary Human Trophoblasts. [Abstract]2021 Aug;28(8):2314-2322. PMID: 33619701 -
Immunol Res
Metformin suppresses gammadelta T17 cell differentiation alleviating DSS-induced colitis. [Abstract]2025 Feb 11;73(1):49. PMID: 39934569 -
Biochem Biophys Res Commun
CD39 is induced by nutrient deprivation in colorectal cancer cells and contributes to the tolerance to hypoxia. [Abstract]2024 Oct 20:730:150367. PMID: 38991255 -
Virus Genes
AMP-activated kinase regulates porcine reproductive and respiratory syndrome virus infection in vitro. [Abstract]2022 Apr;58(2):133-142. PMID: 35254586 -
bioRxiv
Adiponectin Signaling Regulates Urinary Bladder Function by Blunting Smooth Muscle Purinergic Contractility. [Abstract]2024 Oct 29:2024.10.25.620328. PMID: 39554160 -
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Solvente y solubilidad
DMSO : 50 mg/mL (138.74 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
-
+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (290 KB)
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SDS (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Cool B, et al. Identification and characterization of a small molecule AMPK activator that treats key components of type 2 diabetes and the metabolic syndrome. Cell Metab, 2006, 3(6), 403-416. [Content Brief]
[2]. Sanders MJ, et al. Defining the mechanism of activation of AMP-activated protein kinase by the small molecule A-769662, a member of the thienopyridone family. J Biol Chem, 2007, 282(45), 32539-32548. [Content Brief]
[3]. Moreno D, et al, A769662, a novel activator of AMP-activated protein kinase, inhibits non-proteolytic components of the 26S proteasome by an AMPK-independent mechanism. FEBS Lett, 2008, 583(17), 2650-2654. [Content Brief]
[4]. Yerra VG, et al. Adenosine Monophosphate-Activated Protein Kinase Abates Hyperglycaemia-Induced Neuronal Injury in Experimental Models of Diabetic Neuropathy: Effects on Mitochondrial Biogenesis, Autophagy and Neuroinflammation. Mol Neurobiol. 2017 Apr;54(3):2301-2312. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7748 mL | 13.8739 mL | 27.7477 mL | 69.3693 mL |
| 5 mM | 0.5550 mL | 2.7748 mL | 5.5495 mL | 13.8739 mL | |
| 10 mM | 0.2775 mL | 1.3874 mL | 2.7748 mL | 6.9369 mL | |
| 15 mM | 0.1850 mL | 0.9249 mL | 1.8498 mL | 4.6246 mL | |
| 20 mM | 0.1387 mL | 0.6937 mL | 1.3874 mL | 3.4685 mL | |
| 25 mM | 0.1110 mL | 0.5550 mL | 1.1099 mL | 2.7748 mL | |
| 30 mM | 0.0925 mL | 0.4625 mL | 0.9249 mL | 2.3123 mL | |
| 40 mM | 0.0694 mL | 0.3468 mL | 0.6937 mL | 1.7342 mL | |
| 50 mM | 0.0555 mL | 0.2775 mL | 0.5550 mL | 1.3874 mL | |
| 60 mM | 0.0462 mL | 0.2312 mL | 0.4625 mL | 1.1562 mL | |
| 80 mM | 0.0347 mL | 0.1734 mL | 0.3468 mL | 0.8671 mL | |
| 100 mM | 0.0277 mL | 0.1387 mL | 0.2775 mL | 0.6937 mL |
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