1. Dye Reagents

ATP-Red 1 

Cat. No.: HY-U00451 Purity: 98.57%
Handling Instructions

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells.

For research use only. We do not sell to patients.

ATP-Red 1 Chemical Structure

ATP-Red 1 Chemical Structure

CAS No. : 1847485-97-5

Size Price Stock Quantity
5 mg USD 1300 In-stock
Estimated Time of Arrival: December 31
10 mg USD 2400 In-stock
Estimated Time of Arrival: December 31
25 mg USD 4800 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells.

In Vitro

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells. The maximum absorption and emission wavelength of are 570/566 nm and 590/585 nm. ATP-Red 1 has good membrane permeability, and in the presence of 5 mM ATP, the fluorescence intensity of ATP-Red 1 increases 5.6-fold. ATP-Red 1 (2.5 μM, 20 min) shows much weaker fluorescence after KCN-induced inhibition of OXPHOS, which results in reduced mitochondrial ATP levels in OSCC cells[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7810 mL 8.9050 mL 17.8101 mL
5 mM 0.3562 mL 1.7810 mL 3.5620 mL
10 mM 0.1781 mL 0.8905 mL 1.7810 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

OSCC Cells are plated in 96-well flat-bottomed plates at 1×105 cells per well and allowed to grow 3 or 24 h prior to exposure to ATP-Red 1. Then MTT reagent is added for 4 h at 37 °C and DMSO (100 μL/well) is further incubated with cells for 15 min after removing the medium. The absorbance at 570 nm and 690 nm (background signal) is recorded in a Spectra Max M2 microplate reader. The following formula is used to calculate the viability of cell growth: Cell viability (%) = (mean of A value of treatment group / mean of A value of control) × 100[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

561.48

Formula

C₃₄H₃₆BN₃O₄

CAS No.

1847485-97-5

SMILES

O=C1N(C2=CC=CC=C2B(O)O)C3(C4=C(OC5=C3C=CC(N(CC)CC)=C5)C=C(N(CC)CC)C=C4)C6=C1C=CC=C6

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
C1   V1   C2   V2

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Product Name:
ATP-Red 1
Cat. No.:
HY-U00451
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ATP-Red 1

Cat. No.: HY-U00451