ATP-Red 1

Name: ATP-Red 1
Brand: MedChemExpress
SKU: HY-U00451
Rating: 5.0 (9 reviews)

Cat. No.: HY-U00451 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).

For research use only. We do not sell to patients.

CAS No. : 1847485-97-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of ATP-Red 1:

ATP-Red 1 (solution) In-stock

9 Publications Citing Use of MCE ATP-Red 1

Cell Imaging/Staining

: ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2025 Aug;182(16):3923-3951. [Abstract]; ATP production in HeLa cells after BYB (0–15 μM) treatment for 24 h. The ATP was evaluated with ATP-Red 1 (red, λex = 561 nm; 2 μM; 30 min).

: ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Dec 24;26(1):44. [Abstract]; Representative images of ATP-Red 1 staining (red, labeling mitochondrial ATP; 5 μM; 30 min) in shCon-expressing-control,shATAD1-expressing and oe-ATAD1-expressing neuron.

: ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Drug Dev Res. 2025 Sep;86(6):e70150. [Abstract]; ATP‐Red 1 probe (30 min) was used to measure ATP content in H9C2 cells. Sesn2 overexpression increased ATP levels and alleviated the decrease in ATP caused by H/R.

: ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Drug Dev Res. 2025 Sep;86(6):e70150. [Abstract]; ATP‐Red 1 probe (30 min) was used to measure ATP content in H9C2 cells. ML385 was found to reduce ATP content.

: ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Eur J Med Res. 2025 Oct 17;30(1):991. [Abstract]; ATP production level as measured by fluorescence intensity in HK-2 cells by ATP-Red 1.

: ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Cells. 2022 Dec 23;12(1):68.; ATP-Red1 detection of ATP content in SNU-368 under PDE2A-overexpressed treatment and EV as control.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining (suspended cells)
2.1 Collect cells by centrifugation and wash twice with PBS for 5 minutes each time. The cell density is 1×10⁶/mL.
2.2 Add ATP-Red 1 at a final concentration of 5-10 μM.
2.3 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
2.4 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
2.5 Add PBS to wash the cells twice, 5 minutes each time.
2.6 Resuspend the cells of serum-free medium or PBS and observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Culture the adherent cells on a sterile coverslip.
3.2 Remove the coverslip from the culture medium and remove the excess culture medium. 3.3 Add ATP-Red 1 at a final concentration of 5-10 μM.
3.4 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
3.5 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope (Ex/Em = 510/590 nm) or flow cytometer. Note: If flow cytometry is required, the cells need to be digested with trypsin and resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

561.48

Formula

C₃₄H₃₆BN₃O₄

CAS No.

1847485-97-5

Appearance

Solid

Color

Pale purple to purple

Emission (Em)

590

Excitation (Ex)

510

SMILES

O=C1N(C2=CC=CC=C2B(O)O)C3(C4=C(OC5=C3C=CC(N(CC)CC)=C5)C=C(N(CC)CC)C=C4)C6=C1C=CC=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (89.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7810 mL	8.9050 mL	17.8101 mL
5 mM	0.3562 mL	1.7810 mL	3.5620 mL
10 mM	0.1781 mL	0.8905 mL	1.7810 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (285 KB) SDS (252 KB)

COA (263 KB) HNMR (288 KB)

Handling Instructions (2659 KB)

References

[1]. Wang L, et al. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6 [Content Brief]

Cell Assay
^[1]

OSCC Cells are plated in 96-well flat-bottomed plates at 1×10⁵ cells per well and allowed to grow 3 or 24 h prior to exposure to ATP-Red 1. Then MTT reagent is added for 4 h at 37 °C and DMSO (100 μL/well) is further incubated with cells for 15 min after removing the medium. The absorbance at 570 nm and 690 nm (background signal) is recorded in a Spectra Max M2 microplate reader. The following formula is used to calculate the viability of cell growth: Cell viability (%) = (mean of A value of treatment group / mean of A value of control) × 100^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Wang L, et al. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7810 mL	8.9050 mL	17.8101 mL	44.5252 mL
	5 mM	0.3562 mL	1.7810 mL	3.5620 mL	8.9050 mL
	10 mM	0.1781 mL	0.8905 mL	1.7810 mL	4.4525 mL
	15 mM	0.1187 mL	0.5937 mL	1.1873 mL	2.9683 mL
	20 mM	0.0891 mL	0.4453 mL	0.8905 mL	2.2263 mL
	25 mM	0.0712 mL	0.3562 mL	0.7124 mL	1.7810 mL
	30 mM	0.0594 mL	0.2968 mL	0.5937 mL	1.4842 mL
	40 mM	0.0445 mL	0.2226 mL	0.4453 mL	1.1131 mL
	50 mM	0.0356 mL	0.1781 mL	0.3562 mL	0.8905 mL
	60 mM	0.0297 mL	0.1484 mL	0.2968 mL	0.7421 mL
	80 mM	0.0223 mL	0.1113 mL	0.2226 mL	0.5566 mL

ATP-Red 1 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ATP-Red 11847485-97-5Fluorescent DyeInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

ATP-Red 1

Customer Review

Other Forms of ATP-Red 1:

ATP-Red 1 Related Antibodies

9 Publications Citing Use of MCE ATP-Red 1

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

ATP-Red 1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ATP-Red 1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report