ATP-Red 1 (solution)
Based on 1 Customer Validation
ATP-Red 1 (solution) is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).
Solvent and concentration: DMSO: 1 mM
For research use only. We do not sell to patients.
- CAS No.: 1847485-97-5
- Formula: C34H36BN3O4
- Molecular Weight:561.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Solvent and concentration: DMSO: 1 mM
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Disperse and suspend cells in 100 μL PBS in a 96-well plate at a density of 2×105/mL.
2.2 Add ATP-Red 1 at a final concentration of 1-10 μM.
2.3 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
2.4 Remove ATP-Red 1 working solution.
2.5 Analyze with a fluorescence microscope (Ex/Em = 510/590 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1847485-97-5
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Appearance Liquid (Density: 1.309±0.10 g/cm3)
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Molecular Weight 561.48
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Formula C34H36BN3O4
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Color Pale purple to purple
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SMILES
O=C1N(C2(C3=C1C=CC=C3)C4=C(C=C(C=C4)N(CC)CC)OC5=C2C=CC(N(CC)CC)=C5)C6=CC=CC=C6B(O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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