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  4. ATP-Red 1

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).

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CAS 番号 : 1847485-97-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 803 在庫あり
Solution
10 mM * 1 mL in DMSO USD 803 在庫あり
Solid
1 mg $320 在庫あり
5 mg $650 在庫あり
10 mg $1200 在庫あり
25 mg $2400 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 9 publication(s) in Google Scholar

Other Forms of ATP-Red 1:

ATP-Red 1 関連抗体

Top Publications Citing Use of Products

    ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2025 Aug;182(16):3923-3951.  [Abstract]

    ATP production in HeLa cells after BYB (0–15 μM) treatment for 24 h. The ATP was evaluated with ATP-Red 1 (red, λex = 561 nm; 2 μM; 30 min).

    ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Dec 24;26(1):44.  [Abstract]

    Representative images of ATP-Red 1 staining (red, labeling mitochondrial ATP; 5 μM; 30 min) in shCon-expressing-control,shATAD1-expressing and oe-ATAD1-expressing neuron.

    ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Drug Dev Res. 2025 Sep;86(6):e70150.  [Abstract]

    ATP‐Red 1 probe (30 min) was used to measure ATP content in H9C2 cells. Sesn2 overexpression increased ATP levels and alleviated the decrease in ATP caused by H/R.

    ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Drug Dev Res. 2025 Sep;86(6):e70150.  [Abstract]

    ATP‐Red 1 probe (30 min) was used to measure ATP content in H9C2 cells. ML385 was found to reduce ATP content.

    ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Eur J Med Res. 2025 Oct 17;30(1):991.  [Abstract]

    ATP production level as measured by fluorescence intensity in HK-2 cells by ATP-Red 1.

    ATP-Red 1 purchased from MedChemExpress. Usage Cited in: Cells. 2022 Dec 23;12(1):68.

    ATP-Red1 detection of ATP content in SNU-368 under PDE2A-overexpressed treatment and EV as control.

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    製品説明

    ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).

    体外実験

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 10 mM is recommended.
    Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining (suspended cells)
    2.1 Collect cells by centrifugation and wash twice with PBS for 5 minutes each time. The cell density is 1×106/mL.
    2.2 Add ATP-Red 1 at a final concentration of 5-10 μM.
    2.3 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
    2.4 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
    2.5 Add PBS to wash the cells twice, 5 minutes each time.
    2.6 Resuspend the cells of serum-free medium or PBS and observe using a fluorescence microscope or flow cytometer.
    3. Cell staining (adherent cells)
    3.1 Culture the adherent cells on a sterile coverslip.
    3.2 Remove the coverslip from the culture medium and remove the excess culture medium. 3.3 Add ATP-Red 1 at a final concentration of 5-10 μM.
    3.4 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
    3.5 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope (Ex/Em = 510/590 nm) or flow cytometer. Note: If flow cytometry is required, the cells need to be digested with trypsin and resuspended before staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP-Red 1 関連抗体
    分子量

    561.48

    分子式

    C34H36BN3O4
    Emission (Em)

    590
    Excitation (Ex)

    510
    CAS 番号
    Appearance

    Solid

    Color

    Pale purple to purple

    SMILES

    O=C1N(C2=CC=CC=C2B(O)O)C3(C4=C(OC5=C3C=CC(N(CC)CC)=C5)C=C(N(CC)CC)C=C4)C6=C1C=CC=C6
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (89.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7810 mL 8.9050 mL 17.8101 mL
    5 mM 0.3562 mL 1.7810 mL 3.5620 mL
    10 mM 0.1781 mL 0.8905 mL 1.7810 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    純度とドキュメンテーション

    純度: 98.0%

    COA (263 KB) HNMR (288 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [1]

    OSCC Cells are plated in 96-well flat-bottomed plates at 1×105 cells per well and allowed to grow 3 or 24 h prior to exposure to ATP-Red 1. Then MTT reagent is added for 4 h at 37 °C and DMSO (100 μL/well) is further incubated with cells for 15 min after removing the medium. The absorbance at 570 nm and 690 nm (background signal) is recorded in a Spectra Max M2 microplate reader. The following formula is used to calculate the viability of cell growth: Cell viability (%) = (mean of A value of treatment group / mean of A value of control) × 100[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7810 mL 8.9050 mL 17.8101 mL 44.5252 mL
    5 mM 0.3562 mL 1.7810 mL 3.5620 mL 8.9050 mL
    10 mM 0.1781 mL 0.8905 mL 1.7810 mL 4.4525 mL
    15 mM 0.1187 mL 0.5937 mL 1.1873 mL 2.9683 mL
    20 mM 0.0891 mL 0.4453 mL 0.8905 mL 2.2263 mL
    25 mM 0.0712 mL 0.3562 mL 0.7124 mL 1.7810 mL
    30 mM 0.0594 mL 0.2968 mL 0.5937 mL 1.4842 mL
    40 mM 0.0445 mL 0.2226 mL 0.4453 mL 1.1131 mL
    50 mM 0.0356 mL 0.1781 mL 0.3562 mL 0.8905 mL
    60 mM 0.0297 mL 0.1484 mL 0.2968 mL 0.7421 mL
    80 mM 0.0223 mL 0.1113 mL 0.2226 mL 0.5566 mL
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    ATP-Red 1 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP-Red 11847485-97-5Fluorescent DyeInhibitorinhibitorinhibit

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    製品名:
    ATP-Red 1
    製品番号:
    HY-U00451
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