Ac-CoA Synthase-IN-1

Name: Ac-CoA Synthase-IN-1
Brand: MedChemExpress
SKU: HY-104032
Rating: 5.0 (7 reviews)

Cat. No.: HY-104032 Pureza: 99.70%: Ficha de datos
COA

SDS
Instrucciones de manejo
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Ac-CoA Synthase-IN-1 is a potent, reversible acetate-dependent acetyl-CoA synthetase 2 (ACSS2) inhibitor with an IC₅₀ of 0.6 μM. Ac-CoA Synthase-IN-1 inhibits the respiratory syncytial virus (RSV).

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 508186-14-9

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 7 publication(s) in Google Scholar

Other Forms of Ac-CoA Synthase-IN-1:

Ac-CoA Synthase-IN-1 (Standard) Obtener un presupuesto

7 Publications Citing Use of MCE Ac-CoA Synthase-IN-1

•Toxicol Appl Pharmacol. 2022 Dec 15:457:116319. [Abstract]

Others

: Ac-CoA Synthase-IN-1 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 15;14(1):28193. [Abstract]; Ac-CoA Synthase-IN-1 (20 µM). Effect of hypoxia on total lipid content after inhibition of the citrate or acetic acid-dependent pathway. Intracellular lipid accumulation in 3T3-L1 adipocytes after exposure to hypoxia for 7 or 14 days.

: Ac-CoA Synthase-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Nov 7;14(11):722. [Abstract]; Western blot analysis of Ac-CoA Synthase-IN-1 (ACSS2-IN: 5 μM) and PDK1 in Neuro-2a cells after high glucose stimulation.

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Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Ac-CoA Synthase-IN-1 is a potent, reversible acetate-dependent acetyl-CoA synthetase 2 (ACSS2) inhibitor with an IC₅₀ of 0.6 μM^[1]. Ac-CoA Synthase-IN-1 inhibits the respiratory syncytial virus (RSV)^[2].

In Vitro

Ac-CoA Synthase-IN-1 inhibits cellular [¹⁴C]acetate uptake into both lipids and histones with an IC₅₀ of 5 μM. Ac-CoA Synthase-IN-1 inhibits the ability of HepG2 cells to incorporate [¹⁴C]acetate into lipids with IC₅₀ of 6.8 μM. Ac-CoA Synthase-IN-1 inhibits HepG2 utilization of [¹⁴C]acetate for histone acetylation with IC₅₀ of 5.5 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

410.51

Fòrmula

C₂₀H₁₈N₄O₂S₂

No. CAS

508186-14-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NCCOC)NC1=CC=C2N=C(C3=CC=CS3)C(C4=CC=CS4)=NC2=C1

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : 31.25 mg/mL (76.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4360 mL	12.1800 mL	24.3599 mL
5 mM	0.4872 mL	2.4360 mL	4.8720 mL
10 mM	0.2436 mL	1.2180 mL	2.4360 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.70%

Select Batch:

Ficha de datos (273 KB) SDS (396 KB)

COA (248 KB) LCMS (99 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Sarah A Comerford, et al. Acetate dependence of tumors. Cell. 2014 Dec 18;159(7):1591-602. [Content Brief]

[2]. Bill Severson, et al. Anti-viral treatment and assay to screen for anti-viral agent. WO 2011097607 A1

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4360 mL	12.1800 mL	24.3599 mL	60.8999 mL
	5 mM	0.4872 mL	2.4360 mL	4.8720 mL	12.1800 mL
	10 mM	0.2436 mL	1.2180 mL	2.4360 mL	6.0900 mL
	15 mM	0.1624 mL	0.8120 mL	1.6240 mL	4.0600 mL
	20 mM	0.1218 mL	0.6090 mL	1.2180 mL	3.0450 mL
	25 mM	0.0974 mL	0.4872 mL	0.9744 mL	2.4360 mL
	30 mM	0.0812 mL	0.4060 mL	0.8120 mL	2.0300 mL
	40 mM	0.0609 mL	0.3045 mL	0.6090 mL	1.5225 mL
	50 mM	0.0487 mL	0.2436 mL	0.4872 mL	1.2180 mL
	60 mM	0.0406 mL	0.2030 mL	0.4060 mL	1.0150 mL