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  4. Acridine Orange 10-Nonyl Bromide

Acridine Orange 10-Nonyl Bromide  (Synonyms: Nonylacridine orange)

製品番号: HY-D0993 純度: 99.50%
COA 取扱説明書 Technical Support

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

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CAS 番号 : 75168-11-5

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 41 在庫あり
Solution
10 mM * 1 mL in DMSO USD 41 在庫あり
Solid
5 mg $39 在庫あり
10 mg $66 在庫あり
25 mg $140 在庫あり
50 mg $222 在庫あり
100 mg $344 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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Based on 4 publication(s) in Google Scholar

Other Forms of Acridine Orange 10-Nonyl Bromide:

Acridine Orange 10-Nonyl Bromide 関連抗体

Top Publications Citing Use of Products

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557.

    Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin in HeLa cells was detected by employing NAO staining.

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557.

    Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin was detected in Fer-1 (2 μM; 2 h) or Mito-Q (2 μM; 2 h) pretreated HeLa cells or in MFF or DRP1 knockdown HeLa cells.

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557.

    Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin in HeLa cells was detected by NAO staining.

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Chin Med J (Engl). 2024 Sep 3.  [Abstract]

    Acridine Orange 10-Nonyl Bromide (NAO) (5 μM; 30 min) was used to evaluate mitochondrial mass of SCC9 and SCC25 cells after being treated with afatinib (1 μM), KN93 (30 μM), or their combination for 24 h.

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    製品説明

    Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

    体外実験

    Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria. In 15 min at 20°C, Acridine Orange 10-Nonyl Bromide at 4.75 μM saturates the mitochondrial binding sites present in 1.5 X 106 cells[1].
    When Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acridine Orange 10-Nonyl Bromide 関連抗体
    分子量

    472.50

    分子式

    C26H38BrN3
    Emission (Em)

    524
    Excitation (Ex)

    496
    CAS 番号
    Appearance

    Solid

    Color

    Brown to red

    SMILES

    CCCCCCCCC[N+]1=C2C=C(N(C)C)C=CC2=CC3=C1C=C(N(C)C)C=C3.[Br-]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 20 mg/mL (42.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1164 mL 10.5820 mL 21.1640 mL
    5 mM 0.4233 mL 2.1164 mL 4.2328 mL
    10 mM 0.2116 mL 1.0582 mL 2.1164 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション

    純度: 99.50%

    COA (249 KB) HNMR (250 KB) LCMS (256 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    Yeast cells in log phase are fixed in cold ethanol (70% by vol.) and stored at -20°C. Fixed cells are washed three times with cold 10 mM Tris/HCI pH 7, then mildly sonicated to eliminate aggregates and finally counted. Yeast cells are added to 45 μM Acridine Orange 10-Nonyl Bromide and incubated for 15 min at 20°C. Cells are centrifuged (3000×g, 5 min) then washed twice in 10 mM Tris/HCl pH 7. Red fluorescence of Acridine Orange 10-Nonyl Bromide bound to 106 yeast cells is measured at 640 nm and correlated to the calibration curve run with thin-walled vesicles containing known amounts of cardiolipin[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1164 mL 10.5820 mL 21.1640 mL 52.9101 mL
    5 mM 0.4233 mL 2.1164 mL 4.2328 mL 10.5820 mL
    10 mM 0.2116 mL 1.0582 mL 2.1164 mL 5.2910 mL
    15 mM 0.1411 mL 0.7055 mL 1.4109 mL 3.5273 mL
    20 mM 0.1058 mL 0.5291 mL 1.0582 mL 2.6455 mL
    25 mM 0.0847 mL 0.4233 mL 0.8466 mL 2.1164 mL
    30 mM 0.0705 mL 0.3527 mL 0.7055 mL 1.7637 mL
    40 mM 0.0529 mL 0.2646 mL 0.5291 mL 1.3228 mL
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    Acridine Orange 10-Nonyl Bromide Related Classifications

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acridine Orange 10-Nonyl75168-11-5Nonylacridine orangeFluorescent DyeInhibitorinhibitorinhibit

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    製品名:
    Acridine Orange 10-Nonyl Bromide
    製品番号:
    HY-D0993
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