Acridine Orange 10-Nonyl Bromide (solution) 

Acridine Orange 10-Nonyl Bromide (solution) is a fluorescent probe for cardiolipin (λ_ex: 489 nm, λ_em: 525 nm).
Solvent and concentration: DMSO: 10 mM

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λ_ex: 489 nm, λ_em: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria. In 15 min at 20°C, Acridine Orange 10-Nonyl Bromide at 4.75 μM saturates the mitochondrial binding sites present in 1.5 X 10⁶ cells^[1].
When Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

472.52

C₂₆H₃₈BrN₃

75168-11-5

CCCCCCCCC[N+]1=C2C=C(N(C)C)C=CC2=CC3=C1C=C(N(C)C)C=C3.[Br-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12.  [Content Brief]

  [2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9.  [Content Brief]