1. Dye Reagents

Acridine Orange 10-Nonyl Bromide (Synonyms: Nonylacridine orange)

Cat. No.: HY-D0993 Purity: >95.0%
Handling Instructions

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

For research use only. We do not sell to patients.

Acridine Orange 10-Nonyl Bromide Chemical Structure

Acridine Orange 10-Nonyl Bromide Chemical Structure

CAS No. : 75168-11-5

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10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

In Vivo

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].

References
Cell Assay
[2]

Yeast cells in log phase are fixed in cold ethanol (70% by vol.) and stored at -20°C. Fixed cells are washed three times with cold 10 mM Tris/HCI pH 7, then mildly sonicated to eliminate aggregates and finally counted. Yeast cells are added to 45 μM Acridine Orange 10-Nonyl Bromide and incubated for 15 min at 20°C. Cells are centrifuged (3000×g, 5 min) then washed twice in 10 mM Tris/HCl pH 7. Red fluorescence of Acridine Orange 10-Nonyl Bromide bound to 106 yeast cells is measured at 640 nm and correlated to the calibration curve run with thin-walled vesicles containing known amounts of cardiolipin[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

472.50

Formula

C₂₆H₃₈BrN₃

CAS No.

75168-11-5

SMILES

CCCCCCCCC[N+]1=C2C=C(N(C)C)C=CC2=CC3=C1C=C(N(C)C)C=C3.[Br-]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Acridine Orange 10-Nonyl Bromide
Cat. No.:
HY-D0993
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Acridine Orange 10-Nonyl Bromide

Cat. No.: HY-D0993