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  4. Acridine Orange 10-Nonyl Bromide

Acridine Orange 10-Nonyl Bromide  (Synonyms: Nonylacridine orange)

Cat. No.: HY-D0993 Purity: 99.50%
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Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

For research use only. We do not sell to patients.

CAS No. : 75168-11-5

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Acridine Orange 10-Nonyl Bromide:

Acridine Orange 10-Nonyl Bromide Related Antibodies

Top Publications Citing Use of Products

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557.

    Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin in HeLa cells was detected by employing NAO staining.

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557.

    Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin was detected in Fer-1 (2 μM; 2 h) or Mito-Q (2 μM; 2 h) pretreated HeLa cells or in MFF or DRP1 knockdown HeLa cells.

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557.

    Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin in HeLa cells was detected by NAO staining.

    Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Chin Med J (Engl). 2024 Sep 3.  [Abstract]

    Acridine Orange 10-Nonyl Bromide (NAO) (5 μM; 30 min) was used to evaluate mitochondrial mass of SCC9 and SCC25 cells after being treated with afatinib (1 μM), KN93 (30 μM), or their combination for 24 h.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

    In Vitro

    Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria. In 15 min at 20°C, Acridine Orange 10-Nonyl Bromide at 4.75 μM saturates the mitochondrial binding sites present in 1.5 X 106 cells[1].
    When Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acridine Orange 10-Nonyl Bromide Related Antibodies
    Molecular Weight

    472.50

    Formula

    C26H38BrN3
    CAS No.
    Appearance

    Solid

    Color

    Brown to red

    Emission (Em)

    524
    Excitation (Ex)

    496
    SMILES

    CCCCCCCCC[N+]1=C2C=C(N(C)C)C=CC2=CC3=C1C=C(N(C)C)C=C3.[Br-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 20 mg/mL (42.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1164 mL 10.5820 mL 21.1640 mL
    5 mM 0.4233 mL 2.1164 mL 4.2328 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation

    Purity: 99.50%

    SDS (393 KB)

    COA (249 KB) HNMR (250 KB) LCMS (256 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    Yeast cells in log phase are fixed in cold ethanol (70% by vol.) and stored at -20°C. Fixed cells are washed three times with cold 10 mM Tris/HCI pH 7, then mildly sonicated to eliminate aggregates and finally counted. Yeast cells are added to 45 μM Acridine Orange 10-Nonyl Bromide and incubated for 15 min at 20°C. Cells are centrifuged (3000×g, 5 min) then washed twice in 10 mM Tris/HCl pH 7. Red fluorescence of Acridine Orange 10-Nonyl Bromide bound to 106 yeast cells is measured at 640 nm and correlated to the calibration curve run with thin-walled vesicles containing known amounts of cardiolipin[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1164 mL 10.5820 mL 21.1640 mL 52.9101 mL
    5 mM 0.4233 mL 2.1164 mL 4.2328 mL 10.5820 mL
    10 mM 0.2116 mL 1.0582 mL 2.1164 mL 5.2910 mL
    15 mM 0.1411 mL 0.7055 mL 1.4109 mL 3.5273 mL
    20 mM 0.1058 mL 0.5291 mL 1.0582 mL 2.6455 mL
    25 mM 0.0847 mL 0.4233 mL 0.8466 mL 2.1164 mL
    30 mM 0.0705 mL 0.3527 mL 0.7055 mL 1.7637 mL
    40 mM 0.0529 mL 0.2646 mL 0.5291 mL 1.3228 mL
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    Acridine Orange 10-Nonyl Bromide Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acridine Orange 10-Nonyl75168-11-5Nonylacridine orangeFluorescent DyeInhibitorinhibitorinhibit

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