Acridine Orange 10-Nonyl Bromide
Based on 4 publication(s) in Google Scholar
Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).
For research use only. We do not sell to patients.
- Purity: 99.50%
- CAS No.: 75168-11-5
- Formula: C26H38BrN3
- Molecular Weight:472.50
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Acridine Orange 10-Nonyl Bromide
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Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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Flow Cytometry
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Flow Cytometry
Biological Activity
Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria. In 15 min at 20°C, Acridine Orange 10-Nonyl Bromide at 4.75 μM saturates the mitochondrial binding sites present in 1.5 X 106 cells[1].
When Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 75168-11-5
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Appearance Solid
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Molecular Weight 472.50
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Formula C26H38BrN3
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Color Brown to red
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SMILES
CCCCCCCCC[N+]1=C2C=C(N(C)C)C=CC2=CC3=C1C=C(N(C)C)C=C3.[Br-]
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Synonyms
Nonylacridine orange
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
2024 Sep;26(9):1545-1557. PMID: 38997456
Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557. [Abstract]
Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin in HeLa cells was detected by employing NAO staining.
Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557. [Abstract]
Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin was detected in Fer-1 (2 μM; 2 h) or Mito-Q (2 μM; 2 h) pretreated HeLa cells or in MFF or DRP1 knockdown HeLa cells.
Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1545-1557. [Abstract]
Acridine Orange 10-Nonyl Bromide (NAO) (50 nM; 15 min; 37 ℃). The oxidation of cardiolipin in HeLa cells was detected by NAO staining.
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Chin Med J (Engl)
Precision therapy targeting CAMK2 to overcome resistance to EGFR inhibitors in FAT1-mutated oral squamous cell carcinoma. [Abstract]2025 Aug 5;138(15):1853-1865. PMID: 39227322
Acridine Orange 10-Nonyl Bromide purchased from MedChemExpress. Usage Cited in: Chin Med J (Engl). 2025 Aug 5;138(15):1853-1865. [Abstract]
Acridine Orange 10-Nonyl Bromide (NAO) (5 μM; 30 min) was used to evaluate mitochondrial mass of SCC9 and SCC25 cells after being treated with afatinib (1 μM), KN93 (30 μM), or their combination for 24 h.
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Chem Biol Interact
A pivotal role of selective autophagy in mitochondrial quality control: Implications for zinc oxide nanoparticles induced neurotoxicity. [Abstract]2022 Aug 25:363:110003. PMID: 35661739 -
Solvent & Solubility
DMSO : 20 mg/mL (42.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Yeast cells in log phase are fixed in cold ethanol (70% by vol.) and stored at -20°C. Fixed cells are washed three times with cold 10 mM Tris/HCI pH 7, then mildly sonicated to eliminate aggregates and finally counted. Yeast cells are added to 45 μM Acridine Orange 10-Nonyl Bromide and incubated for 15 min at 20°C. Cells are centrifuged (3000×g, 5 min) then washed twice in 10 mM Tris/HCl pH 7. Red fluorescence of Acridine Orange 10-Nonyl Bromide bound to 106 yeast cells is measured at 640 nm and correlated to the calibration curve run with thin-walled vesicles containing known amounts of cardiolipin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12. [Content Brief]
[2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1164 mL | 10.5820 mL | 21.1640 mL | 52.9101 mL |
| 5 mM | 0.4233 mL | 2.1164 mL | 4.2328 mL | 10.5820 mL | |
| 10 mM | 0.2116 mL | 1.0582 mL | 2.1164 mL | 5.2910 mL | |
| 15 mM | 0.1411 mL | 0.7055 mL | 1.4109 mL | 3.5273 mL | |
| 20 mM | 0.1058 mL | 0.5291 mL | 1.0582 mL | 2.6455 mL | |
| 25 mM | 0.0847 mL | 0.4233 mL | 0.8466 mL | 2.1164 mL | |
| 30 mM | 0.0705 mL | 0.3527 mL | 0.7055 mL | 1.7637 mL | |
| 40 mM | 0.0529 mL | 0.2646 mL | 0.5291 mL | 1.3228 mL |
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