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  2. TMRM Perchlorate

TMRM Perchlorate (Synonyms: T668)

Cat. No.: HY-D0984A Purity: >97.0%
Handling Instructions

TMRM Perchlorate is a cell-permeant cationic lipophilic red fluorescent dye (λex=530 nm, λem=592 nm).

For research use only. We do not sell to patients.

TMRM Perchlorate Chemical Structure

TMRM Perchlorate Chemical Structure

CAS No. : 115532-50-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 154 In-stock
Estimated Time of Arrival: December 31
5 mg USD 140 In-stock
Estimated Time of Arrival: December 31
10 mg USD 210 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of TMRM Perchlorate:

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  • Biological Activity

  • Protocol

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  • References

Description

TMRM Perchlorate is a cell-permeant cationic lipophilic red fluorescent dye (λex=530 nm, λem=592 nm).

In Vitro

TMRM Perchlorate is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of TMRM Perchlorate shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM Perchlorate (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%[1]. Exposure of hippocampal cultures to low concentrations of TMRM Perchlorate (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM Perchlorate (1 to 25 µM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM Perchlorate (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 µM) enhance apoptosis (KD = 500 nM)[2].

Storage

4°C, protect from light

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (124.77 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9963 mL 9.9814 mL 19.9629 mL
5 mM 0.3993 mL 1.9963 mL 3.9926 mL
10 mM 0.1996 mL 0.9981 mL 1.9963 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

500.93

Formula

C₂₅H₂₅ClN₂O₇

CAS No.

115532-50-8

SMILES

O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OC.O=Cl(=O)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
TMRM Perchlorate
Cat. No.:
HY-D0984A
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TMRM Perchlorate

Cat. No.: HY-D0984A