1. Dye Reagents

TMRE (Synonyms: Tetramethylrhodamine ethyl ester perchlorate)

Cat. No.: HY-D0985A Purity: >95.0%
Handling Instructions

TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).

For research use only. We do not sell to patients.

TMRE Chemical Structure

TMRE Chemical Structure

CAS No. : 115532-52-0

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Estimated Time of Arrival: December 31
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).

In Vitro

TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm)[1]. Multidirectional dynamic movement of TMRE is observed in epithelial cells and bidirectional dynamic movement is seen in the superficial cortical fiber cells of live bovine lenses. In the epithelium, the movement of TMRE fluorescence is up to 5 μm/min whereas in the superficial cortex the observed movement is up to 18.5 μm/min. The movement of TMRE fluorescence is abolished with treatment of the uncoupler, carbonyl cyanide m-chlorophenylhydrazone (CCCP)[2].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (291.29 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9419 mL 9.7097 mL 19.4194 mL
5 mM 0.3884 mL 1.9419 mL 3.8839 mL
10 mM 0.1942 mL 0.9710 mL 1.9419 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

514.95

Formula

C₂₆H₂₇ClN₂O₇

CAS No.

115532-52-0

SMILES

O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OCC.O=Cl(=O)([O-])=O

Storage

4°C, protect from light

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
TMRE
Cat. No.:
HY-D0985A
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TMRE

Cat. No.: HY-D0985A