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  3. Fluorescent Dye
  4. TMRE

TMRE  (Synonyms: Tetramethylrhodamine ethyl ester perchlorate)

Cat. No.: HY-D0985A Purity: 98.70%
COA Handling Instructions

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

For research use only. We do not sell to patients.

TMRE Chemical Structure

TMRE Chemical Structure

CAS No. : 115532-52-0

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 57 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 57 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 70 In-stock
25 mg USD 100 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 24 publication(s) in Google Scholar

TMRE Related Antibodies

Top Publications Citing Use of Products

    TMRE purchased from MCE. Usage Cited in: Int J Biol Sci. 2021 Jun 26;17(11):2703-2717.  [Abstract]

    When the mitochondrial membrane potential (ΔΨm) is high, TMRE gathers in the mitochondria and produces red-orange fluorescence. HCT116 cells are seeded in 96‐well plates and treated with Tagitinin C. Then cells are incubated for 30 minutes at 37 °C with TMRE (1 µM) in PBS. The excitation wavelength is 540 nm, and the emission wavelength is 595 nm.

    TMRE purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2021 Sep 15;221:112425.  [Abstract]

    AML-12 cells after treated with TDBPP, then TMRE (100 nM) is added to each well. Cells were stained for 30 min. The red fluorescence stained by TMRE represented mitochondria.

    TMRE purchased from MCE. Usage Cited in: FASEB J. 2020 Apr;34(4):5740-5753.  [Abstract]

    Cells are rinsed with D-PBS, and then incubated with TMRE (50 nM) at 37°C for 20 minutes.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    In Vitro

    1.Preparation of TMRE working solution
    1.1Preparation of the stock solution
    Dissolve 1 mg TMRE in DMSO to obtain 5 mM of stock solution.
    1.2 Preparation of TMRE working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
    Note: Please adjust the concentration of TMRE working solution according to the actual situation.
    2.Cell staining
    2.1 Suspension cells (6-well plate)
    a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
    d.Wash twice with PBS, 5 minutes each time.
    e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a.Culture adherent cells on sterile coverslips.
    b.Remove the coverslip from the medium and aspirate excess medium.
    c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
    d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
    Note: If detection by flow cytometry, cells need to be resuspended before staining.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    TMRE Related Antibodies
    Molecular Weight

    514.95

    Appearance

    Solid

    Formula

    C26H27ClN2O7
    CAS No.
    Emission (Em)

    576
    Excitation (Ex)

    550
    SMILES

    O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OCC.O=Cl(=O)([O-])=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 27.78 mg/mL (53.95 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9419 mL 9.7097 mL 19.4194 mL
    5 mM 0.3884 mL 1.9419 mL 3.8839 mL
    10 mM 0.1942 mL 0.9710 mL 1.9419 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 98.70%

    COA (256 KB) HNMR (244 KB) LCMS (266 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay
    [1]

    The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    TMRE Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    TMRE115532-52-0Tetramethylrhodamine ethyl esterFluorescent DyeInhibitorinhibitorinhibit

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