2. Metabolic Enzyme/Protease
  3. Xanthine Oxidase
  4. Allopurinol

Allopurinol 

Cat. No.: HY-B0219 Purity: 99.96%
COA Handling Instructions

Allopurinol is a potent and orally active xanthine oxidase inhibitor with an IC50 value of 0.2-50 μM. Allopurinol can be used in the research of hyperuricemia and gout. Allopurinol decreases the expression of HIF-1α and HIF-2α protein. Allopurinol shows anti-depressant and anti-nociception activity. Anti-leishmanial effect.

For research use only. We do not sell to patients.

Allopurinol Chemical Structure

Allopurinol Chemical Structure

CAS No. : 315-30-0

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
500 mg USD 55 In-stock
5 g USD 66 In-stock
10 g USD 79 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Allopurinol:

Allopurinol Related Antibodies

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Description

Allopurinol is a potent and orally active xanthine oxidase inhibitor with an IC50 value of 0.2-50 μM. Allopurinol can be used in the research of hyperuricemia and gout. Allopurinol decreases the expression of HIF-1α and HIF-2α protein. Allopurinol shows anti-depressant and anti-nociception activity. Anti-leishmanial effect[1][2][3][4][5].

In Vitro

Allopurinol (0, 10, 100, 1000 µg/ml; 17 h) decreases the expression of HIF-1α and HIF-2α protein in HFF and HUVEC cells[5].
Allopurinol (0, 10, 100, 1000 µg/ml; 24 h) reduces angiogenesis traits of HUVEC cells[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Allopurinol Related Antibodies

Western Blot Analysis[5]

Cell Line: HFF, HUVEC cells
Concentration: 0, 10, 100, 1000 µg/ml
Incubation Time: 17 h
Result: Reduced HIF-1α and HIF-2α protein expression in a dose dependent manner.
In Vivo

Allopurinol (39 mg/kg; p.o.; daily for 21 successive days) shows anti-depressant activity in mouse[3].
Allopurinol (10-400 mg/kg; i.p.) induces anti-nociception activity in mouse[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 20-30 g, male Swiss Albino mice[3]
Dosage: 39 mg/kg
Administration: P.o.; daily for 21 successive days
Result: Reduced the immobility time in the FST with the immobility time of 129.8±10.5 s.
Animal Model: 30-40 g, male adult Swiss albino mice[4]
Dosage: 10, 50, 100, 200, 400 mg/kg
Administration: I.p.
Result: Produced dose-dependent anti-nociception in the tail-flick, hot-plate.
Clinical Trial
Molecular Weight

136.11

Appearance

Solid

Formula

C5H4N4O
CAS No.
SMILES

O=C1C2=C(NN=C2)N=CN1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 14 mg/mL (102.86 mM; Need ultrasonic and warming)

H2O : 1 mg/mL (7.35 mM; ultrasonic and adjust pH to 11 with NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.3470 mL 36.7350 mL 73.4700 mL
5 mM 1.4694 mL 7.3470 mL 14.6940 mL
10 mM 0.7347 mL 3.6735 mL 7.3470 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.33 mg/mL (24.47 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 3.33 mg/mL (24.47 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.61 mg/mL (4.48 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.96%

COA (250 KB) HNMR (251 KB) RP-HPLC (243 KB) MS (69 KB)

Handling Instructions (2659 KB)
References
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Allopurinol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Allopurinol315-30-0Xanthine OxidaseXOorally activehyperuricemiagoutHIF-1αHIF-2αanti-depressantanti-nociceptionanti-leishmanialHUVEC cellsInhibitorinhibitorinhibit

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