Allopurinol

Name: Allopurinol
Brand: MedChemExpress
SKU: HY-B0219
Rating: 5.0 (12 reviews)

Cat. No.: HY-B0219 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Allopurinol is a potent and orally active xanthine oxidase inhibitor with an IC₅₀ value of 0.2-50 μM. Allopurinol can be used in the research of hyperuricemia and gout. Allopurinol decreases the expression of HIF-1α and HIF-2α protein. Allopurinol shows anti-depressant and anti-nociception activity. Anti-leishmanial effect.

For research use only. We do not sell to patients.

CAS No. : 315-30-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Allopurinol:

12 Publications Citing Use of MCE Allopurinol

Histological Imaging/Staining

IHC

RT-PCR

Cell Imaging/Staining

: Allopurinol purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 May 13;31(1):184. [Abstract]; Allopurinol (10 mg/kg/day; oral gavage; once daily for 4 weeks) can significantly reduce the elevated IL-6 levels in the hearts of hyperuricemic mice.

: Allopurinol purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 May 13;31(1):184. [Abstract]; Allopurinol (10 mg/kg/day; oral gavage; once daily for 4 weeks) effectively reduced the phosphorylation of JAK2 and STAT3 and decreased the expression of HMGCS2 protein in the hearts of hyperuricemic mice.

: Allopurinol purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 May 13;31(1):184. [Abstract]; Representative transmission electron microscopy images of mitochondria in cardiomyocytes. Scale bar: 500 nm. Electron microscopy revealed severe mitochondrial damage in cardiomyocytes of hyperuricemic mice, which was normalized by treatment with Allopurinol (10 mg/kg/day; oral gavage; once daily for 4 weeks).

: Allopurinol purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Sep 3:e2400684. [Abstract]; Quantitative analyses of histopathological results reveal a notable increase in collagen volume fraction (9.95 ± 0.50) and oil red area fraction (10.06 ± 0.64) in CP mice treated with WD + Hx, meanwhile NAC (collagen volume fraction: 5.29 ± 0.60; oil red area fraction: 0.08 ± 0.01) and Allopurinol (Allo, 2 g/L in the drinking water and a Western diet for 8 weeks) treatment (collagen volume fraction: 4.63 ± 0.36; oil red area fraction: 0.06 ± 0.001) induced remarkable alleviation in parameters above.

: Allopurinol purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Sep 3:e2400684. [Abstract]; In the target organs (liver and brain cortex), Hx treatment suppressed the relative mRNA expression of critical enzymes in purine metabolism (HPRT, XOR, and PNP) as well as crucial players in anti‐oxidative system (Nrf2, SOD‐1, and CAT), meanwhile, NAC and Allopurinol (Allo, 2 g/L in the drinking water and a Western diet for 8 weeks) treatment induced significant upregulation in relative expression of these genes.

: Allopurinol purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Jun 15:177:116859. [Abstract]; Allopurinol (5 mg/kg; p.o.; single dose) significantly reduces the mRNA levels of XOD in HUA mice.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
L02	CC₅₀	6.842 μM Compound: Allopurinol	Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 35902063]
MRC5	CC₅₀	> 64 μM Compound: Allopurinol	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 3 days by resazurin dye based fluorescence analysis Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 3 days by resazurin dye based fluorescence analysis	[PMID: 35533570]
Peritoneal macrophage	CC₅₀	> 64 μM Compound: 1	Cytotoxicity against Swiss mouse peritoneal macrophages assessed as cell detachment, lysis and granulation measured after 5 days by microscopic analysis Cytotoxicity against Swiss mouse peritoneal macrophages assessed as cell detachment, lysis and granulation measured after 5 days by microscopic analysis	[PMID: 33784107]
Peritoneal macrophage	CC₅₀	> 64 μM Compound: Allopurinol	Cytotoxicity against Swiss mouse peritoneal macrophages assessed as reduction in cell viability measured after 5 days by microscopic analysis Cytotoxicity against Swiss mouse peritoneal macrophages assessed as reduction in cell viability measured after 5 days by microscopic analysis	[PMID: 35533570]
Vero	CC₅₀	428 μM Compound: R8	Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay	[PMID: 27933947]
Vero	IC₅₀	23.9 μM Compound: R8	Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells assessed as inhibition of Stx-induced cytotoxicity pre-treated with compound for 1 hr followed by Stx exposure for 24 hrs by neutral red uptake assay Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells assessed as inhibition of Stx-induced cytotoxicity pre-treated with compound for 1 hr followed by Stx exposure for 24 hrs by neutral red uptake assay	[PMID: 27933947]
Vero	IC₅₀	9.62 μM Compound: 1, allop, allopurinol	Antimicrobial activity against Trypanosoma cruzi RA amastigotes infected in African green monkey Vero cells assessed as growth inhibition after 72 hrs by Giemsa staining-based light microscopy Antimicrobial activity against Trypanosoma cruzi RA amastigotes infected in African green monkey Vero cells assessed as growth inhibition after 72 hrs by Giemsa staining-based light microscopy	[PMID: 24090917]

In Vitro

Allopurinol (0, 10, 100, 1000 μg/ml; 17 h) decreases the expression of HIF-1α and HIF-2α protein in HFF and HUVEC cells^[5].
Allopurinol (0, 10, 100, 1000 μg/ml; 24 h) reduces angiogenesis traits of HUVEC cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[5]

Cell Line:	HFF, HUVEC cells
Concentration:	0, 10, 100, 1000 µg/ml
Incubation Time:	17 h
Result:	Reduced HIF-1α and HIF-2α protein expression in a dose dependent manner.

In Vivo

Allopurinol (39 mg/kg; p.o.; daily for 21 successive days) shows anti-depressant activity in mouse^[3].
Allopurinol (10-400 mg/kg; i.p.) induces anti-nociception activity in mouse^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	20-30 g, male Swiss Albino mice^[3]
Dosage:	39 mg/kg
Administration:	P.o.; daily for 21 successive days
Result:	Reduced the immobility time in the FST with the immobility time of 129.8±10.5 s.

Animal Model:	30-40 g, male adult Swiss albino mice^[4]
Dosage:	10, 50, 100, 200, 400 mg/kg
Administration:	I.p.
Result:	Produced dose-dependent anti-nociception in the tail-flick, hot-plate.

Clinical Trial

Molecular Weight

136.11

Formula

C₅H₄N₄O

CAS No.

315-30-0

Appearance

Solid

Color

White to off-white

SMILES

O=C1C2=C(NN=C2)N=CN1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 14 mg/mL (102.85 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (7.35 mM; ultrasonic and adjust pH to 11 with NaOH)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.3468 mL	36.7339 mL	73.4678 mL
5 mM	1.4694 mL	7.3468 mL	14.6936 mL
10 mM	0.7347 mL	3.6734 mL	7.3468 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.33 mg/mL (24.46 mM); Clear solution

This protocol yields a clear solution of ≥ 3.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.33 mg/mL (24.46 mM); Clear solution

This protocol yields a clear solution of ≥ 3.33 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.61 mg/mL (4.48 mM); Clear solution

This protocol yields a clear solution of ≥ 0.61 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (280 KB) SDS (480 KB)

COA (250 KB) HNMR (131 KB) RP-HPLC (202 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Pacher P, et al. Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacol Rev. 2006 Mar;58(1):87-114. [Content Brief]

[2]. Pfaller MA, et al. Antileishmanial effect of allopurinol. Antimicrob Agents Chemother. 1974;5(5):469-472. [Content Brief]

[3]. Karve AV, et al. Evaluation of effect of allopurinol and febuxostat in behavioral model of depression in mice. Indian J Pharmacol. 2013 May-Jun;45(3):244-7. [Content Brief]

[4]. Schmidt AP, et al. Anti-nociceptive properties of the xanthine oxidase inhibitor allopurinol in mice: role of A1 adenosine receptors. Br J Pharmacol. 2009 Jan;156(1):163-72. [Content Brief]

[5]. Sun Y, et al. Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia. PLoS One. 2015 Apr 1;10(4):e0123649. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	7.3468 mL	36.7339 mL	73.4678 mL	183.6696 mL
H₂O / DMSO	5 mM	1.4694 mL	7.3468 mL	14.6936 mL	36.7339 mL
DMSO	10 mM	0.7347 mL	3.6734 mL	7.3468 mL	18.3670 mL
	15 mM	0.4898 mL	2.4489 mL	4.8979 mL	12.2446 mL
	20 mM	0.3673 mL	1.8367 mL	3.6734 mL	9.1835 mL
	25 mM	0.2939 mL	1.4694 mL	2.9387 mL	7.3468 mL
	30 mM	0.2449 mL	1.2245 mL	2.4489 mL	6.1223 mL
	40 mM	0.1837 mL	0.9183 mL	1.8367 mL	4.5917 mL
	50 mM	0.1469 mL	0.7347 mL	1.4694 mL	3.6734 mL
	60 mM	0.1224 mL	0.6122 mL	1.2245 mL	3.0612 mL
	80 mM	0.0918 mL	0.4592 mL	0.9183 mL	2.2959 mL
	100 mM	0.0735 mL	0.3673 mL	0.7347 mL	1.8367 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Allopurinol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Allopurinol315-30-0Xanthine OxidaseXOorally activehyperuricemiagoutHIF-1αHIF-2αanti-depressantanti-nociceptionanti-leishmanialHUVEC cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Allopurinol

Customer Review

Other Forms of Allopurinol:

Allopurinol Related Antibodies

12 Publications Citing Use of MCE Allopurinol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Allopurinol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Allopurinol Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report