2. Induced Disease Models Products Cell Cycle/DNA Damage
  3. Cancer Models DNA Alkylator/Crosslinker
  4. Colon Cancer Models
  5. Azoxymethane

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

For research use only. We do not sell to patients.

CAS No. : 25843-45-2

Size Price Stock Quantity
Solvent
5 mg (135 mM * 500 μL in Water) In-stock
Solvent
10 mg (135 mM * 1 mL in Water) In-stock
Solvent
25 mg (135 mM * 2.5 mL in Water) In-stock
Solvent
50 mg (135 mM * 5 mL in Water) In-stock
Solvent
100 mg (135 mM * 10 mL in Water) In-stock

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Based on 17 publication(s) in Google Scholar

Azoxymethane Related Antibodies

Top Publications Citing Use of Products

    Azoxymethane purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 8:e2500271.  [Abstract]

    H&E staining  in AOM (10 mg/kg; sc; Seven days later, 2.5% dextran sulfate sodium, was administered in the drinking water for 7 days, followed by 2 weeks of regular drinking water, This DSS treatment cycle was repeated twice for a total of three cycles)/DSS‐induced tumor‐bearing mice treated with DMSO, PRMT5i (40 mg kg−1), CPT‐11 (40 mg kg−1), or PRMT5i + CPT‐11.

    Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329.  [Abstract]

    Immunohistochemical staining showing the histological changes in colon tissues upon OTSSP167 treatment in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice.

    Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329.  [Abstract]

    Immunohistochemistry revealed the expression of ferroptosis related indicators, including 4HNE and MPO treated in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice

    Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329.  [Abstract]

    Differences in the expression of COX2 and SLC39A14 in colon tissues treated with or without OTSSP167 in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice.

    Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329.  [Abstract]

    Changes in the secretion of proinflammatory factors, including IL-1β, IL-6 and TNF-α , induced by the OTSSP167 treatment in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice.

    Azoxymethane purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2020 Nov;61:103068.  [Abstract]

    HE staining of normal epithelium and AOM-DSS (a single dose of AOM (12 mg/kg); ip; fter 3 days, 2% DSS dissolved in the drinking water was administered for 6 days, followed by 15 days of regular drinking water) induced colorectal carcinoma in WT and Ndrg2DIEC mice. Representative images from WT (n=24) and Ndrg2DIEC (n=26) mice.

    • Biological Activity

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    • References

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    Description

    Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

    In Vitro

    Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts. On an equal protein basis, hepatic microsomes are much more active than SI and colon microsomes in NADPH-dependent Azoxymethane bioactivation and N7-mG adduct formation. Hepatic microsomes show the highest activity in the hydroxylation of Azoxymethane, followed by SI and colon microsomes[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Azoxymethane Related Antibodies
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Azoxymethane can be used to induce colorectal cancer models.

    Induction of Colorectal Cancer
    Background
    Azoxymethane induces induces G to A transitions and leads to activating mutations in K-ras and β-catenin in colonocytes, ctivation of Wnt signaling by accumulation of beta-catenin is a major mechanism in the azoxymethane-induced colon carcinogenesis model.
    Specific Modeling Methods
    Mice: 20-25 g•Male A/J mice&bull
    Administration: 5 mg/kg• i.p.• weekly for 6 weeks
    Modeling Indicators
    Cellular/tissue level: Induced hyperproliferation and microadenoma formation, Increased the cyclin D1 and COX-2 expression.
    Molecular changes: Increased the protein expression of pro-TGF-α, pEGFR, pErbB2, pERK, induces K-ras mutations.
    Opposite Product(s): Gefitinib (HY-50895)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    74.08

    Formula

    C2H6N2O
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    C/[N+]([O-])=N/C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, 2 years
    Solvent & Solubility
    In Vitro: 

    H2O : 138.89 mg/mL (1874.87 mM; Need ultrasonic)

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥99.0%

    SDS (795 KB)

    COA (247 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    The assay for Azoxymethane-induced in vitro DNA adduct formation is performed. Briefly, microsomes (0.5 to 2.0 mg/mL) are incubated with calf thymus DNA (1 mg/mL) and Azoxymethane (200 μM) in a total volume of 1.0 mL. The assay buffer consists of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl2, 0.3 M KCl, and 1.5 mM NADPH. Incubations are carried out at 37°C for 60 min in a shaking water bath. An additional 30 nM of NADPH is added after the first 30 min. The reaction is stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA is then extracted for tissue homogenates. Control incubations are performed without NADPH[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Male, 8 to 10 week old, WT-A/J, IECN-A/J, and LCN-A/J mice (8 per group) are treated with either saline or Azoxymethane (7.5 mg/kg BW, s.c.), once weekly for 3 weeks. Mice are sacrificed 6 weeks post-treatment for aberrant crypt foci (ACF) detection. The entire colon is excised. A longitudinal incision is made along the entire length of the colon, which is further cut into two equal-length segments, representing proximal and distal portions of the colon. The segments are dipped in PBS to remove fecal pellets and then kept flat between filter papers in 10% buffered formalin for at least 24 h. Subsequently, the colons are immersed in freshly prepared 0.1% methylene blue for 10 min and rinsed briefly in deionized H2O to remove excess dye. The colon is mounted carefully on a microscope slide with the mucosal surface side up and viewed under a light microscope. The ACF in the entire mucosal surface of the colon are counted blindly and independently by two investigators and recorded[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 13.4989 mL 67.4946 mL 134.9892 mL 337.4730 mL
    5 mM 2.6998 mL 13.4989 mL 26.9978 mL 67.4946 mL
    10 mM 1.3499 mL 6.7495 mL 13.4989 mL 33.7473 mL
    15 mM 0.8999 mL 4.4996 mL 8.9993 mL 22.4982 mL
    20 mM 0.6749 mL 3.3747 mL 6.7495 mL 16.8736 mL
    25 mM 0.5400 mL 2.6998 mL 5.3996 mL 13.4989 mL
    30 mM 0.4500 mL 2.2498 mL 4.4996 mL 11.2491 mL
    40 mM 0.3375 mL 1.6874 mL 3.3747 mL 8.4368 mL
    50 mM 0.2700 mL 1.3499 mL 2.6998 mL 6.7495 mL
    60 mM 0.2250 mL 1.1249 mL 2.2498 mL 5.6245 mL
    80 mM 0.1687 mL 0.8437 mL 1.6874 mL 4.2184 mL
    100 mM 0.1350 mL 0.6749 mL 1.3499 mL 3.3747 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Azoxymethane Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Azoxymethane25843-45-2AOMDNA Alkylator/CrosslinkerInhibitorinhibitorinhibit

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