2. Metabolic Enzyme/Protease
  3. Oxidative Phosphorylation Mitochondrial Metabolism
  4. BAM 15

BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler.

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CAS 番号 : 210302-17-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 在庫あり
Solution
10 mM * 1 mL in DMSO USD 82 在庫あり
Solid
5 mg $75 在庫あり
10 mg $135 在庫あり
25 mg $250 在庫あり
50 mg $400 在庫あり
100 mg $600 在庫あり
250 mg $1000 在庫あり
500 mg $1350 在庫あり
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カスタマーレビュー

Based on 11 publication(s) in Google Scholar

BAM 15 関連抗体

Top Publications Citing Use of Products

    BAM 15 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 Dec 30:101890.  [Abstract]

    BAM 15 (16 h) effectively decreased SKP2 expression in prostate cancer cells.

    BAM 15 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Sep 25:221:107973.  [Abstract]

    H+ current densities at −160 mV in heart mitoplasts with different BAM 15 (0.25-5 µM) concentrations.

    BAM 15 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Sep 25:221:107973.  [Abstract]

    Representative time course of the changes in fluorescence evoked by BAM 15 (500 nM) in WT C2C12 cells expressing SE-pHluorin targeted to the mitochondrial matrix. Mitochondrial matrix acidification due to BAM15 induced alkalinization.

    BAM 15 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Res. 2025 Apr 22.  [Abstract]

    OCR measurement in control and MLN4924-treated activated human CD8+ T cells use BAM 15 (2.5 μmol/L) to induce maximal respiration.

    BAM 15 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2022 Apr:198:114948.  [Abstract]

    Four leukemia cell lines, U937, Kasumi1, THP1, and C1498, were incubated with BAM 15 (0-10 μM; 72 h) on a 96-well plate and the activity changes of the cell lines were measured by the CCK-8 method.

    BAM 15 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2022 Apr:198:114948.  [Abstract]

    The infiltration potential of leukemia cells in the drug-added and vehicle groups was evaluated by H&E staining. Tumor cell invasion in the BAM 15 (5 mg/kg; i.p.; Once every two days; three weeks) group was significantly reduced.

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    製品説明

    BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler[1].

    Cellular Effect
    Cell Line Type Value Description References
    L6 EC50
    0.27 μM
    Compound: BAM15
    Induction of mitochondrial uncoupling in rat L6 cells assessed as stimulation of cellular oxygen consumption
    Induction of mitochondrial uncoupling in rat L6 cells assessed as stimulation of cellular oxygen consumption
    		[PMID: 26119501]
    L6 EC50
    0.31 μM
    Compound: 8; BAM15
    Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay
    Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay
    		[PMID: 32392051]
    L6 EC50
    0.31 μM
    Compound: BAM15
    Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer
    Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer
    		[PMID: 35907607]
    L6 EC50
    0.49 μM
    Compound: 5; BAM15
    Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay
    Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay
    		[PMID: 32017849]
    MCF7 EC50
    0.31 μM
    Compound: BAM15
    Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 33569941]
    MDA-MB-231 IC50
    1.4 μM
    Compound: 179
    Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay
    Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay
    		[PMID: 37300915]
    体外実験

    BAM 15 is able to increase O2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1 μM increase cellular O2 consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1 μM to 50 μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BAM 15 関連抗体
    体内実験

    Compare to vehicle-treated mice, animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia, reduced tubular necrosis, less depletion of brush border villi, less obstruction of proximal tubules, and less immune cell infiltration[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    340.29

    分子式

    C16H10F2N6O
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    FC(C=CC=C1)=C1NC2=NC3=NON=C3N=C2NC4=C(C=CC=C4)F
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (146.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9387 mL 14.6933 mL 29.3867 mL
    5 mM 0.5877 mL 2.9387 mL 5.8773 mL
    10 mM 0.2939 mL 1.4693 mL 2.9387 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

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    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
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    +
    %
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    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.82%

    COA (253 KB) HNMR (310 KB) RP-HPLC (244 KB) LCMS (100 KB)

    取扱説明書 (2659 KB)
    参考文献
    キナーゼ実験
    [1]

    Electron flow assays are performed. Briefly, 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay, mitochondria are incubated at 37°C for 10 mins in MAS containing 10 mM pyruvate, 2 mM malate, and 5 μM BAM 15 or FCCP. Rotenone (2 μM), succinate (10 mM), antimycin A (4 μM), and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD, 100 μM) plus ascorbate (10 mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    細胞実験
    [1]

    L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM, 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×105 cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [1]

    Male mice (8-week old, C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5 mg/kg, 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400)[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9387 mL 14.6933 mL 29.3867 mL 73.4667 mL
    5 mM 0.5877 mL 2.9387 mL 5.8773 mL 14.6933 mL
    10 mM 0.2939 mL 1.4693 mL 2.9387 mL 7.3467 mL
    15 mM 0.1959 mL 0.9796 mL 1.9591 mL 4.8978 mL
    20 mM 0.1469 mL 0.7347 mL 1.4693 mL 3.6733 mL
    25 mM 0.1175 mL 0.5877 mL 1.1755 mL 2.9387 mL
    30 mM 0.0980 mL 0.4898 mL 0.9796 mL 2.4489 mL
    40 mM 0.0735 mL 0.3673 mL 0.7347 mL 1.8367 mL
    50 mM 0.0588 mL 0.2939 mL 0.5877 mL 1.4693 mL
    60 mM 0.0490 mL 0.2449 mL 0.4898 mL 1.2244 mL
    80 mM 0.0367 mL 0.1837 mL 0.3673 mL 0.9183 mL
    100 mM 0.0294 mL 0.1469 mL 0.2939 mL 0.7347 mL
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    BAM 15 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BAM 15210302-17-3BAM15BAM-15Oxidative PhosphorylationMitochondrial MetabolismInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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