1. GPCR/G Protein
  2. CRFR
  3. CRF, bovine

CRF, bovine (Synonyms: Corticotropin Releasing Factor bovine)

Cat. No.: HY-P1533
Handling Instructions

CRF, bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CRF, bovine Chemical Structure

CRF, bovine Chemical Structure

CAS No. : 92307-52-3

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Description

CRF, bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM.

IC50 & Target

Ki: 3.52 nM (CRF receptor)[1]

In Vitro

CRF, bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM[1]. CRF shows pEC50s of 11.16, 8.53 and 8.70 for human CRF1, human CRF2 and rat CRF[2]. CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress, and leads to production of glucocorticoids which down regulate immune responses. CRF also has proinflammatory effects. CRF affects brain microvessel endothelial cells (BMEC) structure or function, CRF (100 nM) significantly increases cAMP in BMEC[3].

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Cell Assay
[3]

CRF (1 μM) is added to the cell cultures that are further incubated for 5, 15 and 30 min at 37°C. Control cells are incubated with medium only. Following incubation time, cells are lysed directly on the growth dish using the detergent provided by the cAMP enzyme immunoassay kit. Following trypan blue staining to ensure complete lysis, the cell lysate is collected and assayed for cAMP. In some cases, the brain microvessel endothelial cells (BMEC) are treated with forskolin or pretreated either with the CRFR antagonist Antalarmin (1 μM) or the ATP analogue 2′5′-deoxyadenosine for 5 min at 37°C[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

4697.34

Formula

C₂₀₆H₃₄₀N₆₀O₆₃S

CAS No.

92307-52-3

Sequence

Ser-Gln-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Thr-Lys-Ala-Asp-Gln-Leu-Ala-Gln-Gln-Ala-His-Asn-Asn-Arg-Lys-Leu-Leu-Asp-Ile-Ala-NH2

Sequence Shortening

SQEPPISLDLTFHLLREVLEMTKADQLAQQAHNNRKLLDIA-NH2

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
CRF, bovine
Cat. No.:
HY-P1533
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CRF, bovine

Cat. No.: HY-P1533