DAPI dihydrochloride (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride)

Name: DAPI dihydrochloride
Brand: MedChemExpress
SKU: HY-D0814
Rating: 5.0 (141 reviews)

Cat. No.: HY-D0814 Purity: 99.90%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).

For research use only. We do not sell to patients.

CAS No. : 28718-90-3

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 141 publication(s) in Google Scholar

Other Forms of DAPI dihydrochloride:

DAPI dihydrochloride (solution) In-stock
DAPI dilactate In-stock
DAPI In-stock

141 Publications Citing Use of MCE DAPI dihydrochloride

Cell Imaging/Staining

: DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620. [Abstract]; Effects of silencing and overexpression of vimentin on apoptosis of MH7A cells. TMRM/DAPI staining (n = 4).

: DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2022 Jun 6;15(6):721.; The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

: DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123. [Abstract]; Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

: DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952. [Abstract]; Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

: DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15:96:468-479. [Abstract]; CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

: DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146. [Abstract]; MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells^[1]. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment^[2] (Ex/Em = 356/451 nm).

In Vitro

Guidelines (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
1. Preparation of DAPI dihydrochloride working solution
Dilute the stock solution with serum-free cell culture medium or PBS to obtain a working solution of 1-10 μg/mL.
Note: Please adjust the concentration of the DAPI dihydrochloride working solution according to your needs.
2. Cell staining
2.1 Suspension cells (96-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes, then discard the supernatant. Wash twice with PBS for 5 minutes each time. Adjust the cell density to 1×10⁶/mL.
b. Add 1 mL working solution and incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and discard the supernatant.
d. Wash twice with PBS for 5 minutes each time.
e. Resuspend the cells in serum-free cell culture medium or PBS. Observe using a fluorescence microscope or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslips from the culture medium and aspirate excess medium.
c. Add 100 μL working solution, gently shake to fully cover the cells, and incubate at room temperature for 3-10 minutes.
d. Wash twice with culture medium for 5 minutes each time. Observe using a fluorescence microscope or flow cytometer.

Storage
Store at -20°C for 1 year. Protect from light.

Notes 1. Please adjust the concentration of the DAPI dihydrochloride working solution according to actual conditions.
2. This product is for R&D use only and is not intended for use in drugs, household, or other purposes.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

350.25

Formula

C₁₆H₁₇Cl₂N₅

CAS No.

28718-90-3

Appearance

Solid

Color

Light yellow to green yellow

Emission (Em)

451

Excitation (Ex)

356

SMILES

N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 75 mg/mL (214.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5.56 mg/mL (15.87 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8551 mL	14.2755 mL	28.5510 mL
5 mM	0.5710 mL	2.8551 mL	5.7102 mL
10 mM	0.2855 mL	1.4276 mL	2.8551 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (274 KB) SDS (394 KB)

COA (247 KB) HNMR (276 KB) RP-HPLC (261 KB) MS (217 KB)

Handling Instructions (2659 KB)

Dyeing Example

References

[1]. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33. [Content Brief]

[2]. Kuduk SD, et al. Amidine derived inhibitors of acid-sensing ion channel-3 (ASIC3). Bioorg Med Chem Lett. 2009 Aug 1;19(15):4059-63. Sep;70(5):220-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.8551 mL	14.2755 mL	28.5510 mL	71.3776 mL
	5 mM	0.5710 mL	2.8551 mL	5.7102 mL	14.2755 mL
	10 mM	0.2855 mL	1.4276 mL	2.8551 mL	7.1378 mL
	15 mM	0.1903 mL	0.9517 mL	1.9034 mL	4.7585 mL
DMSO	20 mM	0.1428 mL	0.7138 mL	1.4276 mL	3.5689 mL
	25 mM	0.1142 mL	0.5710 mL	1.1420 mL	2.8551 mL
	30 mM	0.0952 mL	0.4759 mL	0.9517 mL	2.3793 mL
	40 mM	0.0714 mL	0.3569 mL	0.7138 mL	1.7844 mL
	50 mM	0.0571 mL	0.2855 mL	0.5710 mL	1.4276 mL
	60 mM	0.0476 mL	0.2379 mL	0.4759 mL	1.1896 mL
	80 mM	0.0357 mL	0.1784 mL	0.3569 mL	0.8922 mL
	100 mM	0.0286 mL	0.1428 mL	0.2855 mL	0.7138 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

DAPI dihydrochloride Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAPI28718-90-34',6-Diamidino-2-phenylindoleDNA StainSodium ChannelNa channelsNa+ channelsInhibitorinhibitorinhibit

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DAPI dihydrochloride (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride)

Customer Review

Other Forms of DAPI dihydrochloride:

DAPI dihydrochloride Related Antibodies

141 Publications Citing Use of MCE DAPI dihydrochloride

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•Related Small Molecules:

•Related Proteins:

View All Sodium Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

DAPI dihydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DAPI dihydrochloride Related Classifications

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