2. Cell Cycle/DNA Damage Membrane Transporter/Ion Channel
  3. DNA Stain Sodium Channel
  4. DAPI dihydrochloride

DAPI (dihydrochloride)  (Synonyms: DAPI 2HCl)

製品番号: HY-D0814 純度: 99.90%
COA 取扱説明書 Technical Support

DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 28718-90-3

容量 価格（税別） 在庫状況 数量
1 mg $30 在庫あり
5 mg $46 在庫あり
10 mg $78 在庫あり
25 mg $163 在庫あり
50 mg $260 在庫あり
100 mg   お問い合わせ  
200 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 141 publication(s) in Google Scholar

Other Forms of DAPI dihydrochloride:

DAPI (dihydrochloride) 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Imaging/Staining
IF

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620.  [Abstract]

    Effects of silencing and overexpression of vimentin on apoptosis of MH7A cells. TMRM/DAPI staining (n = 4).

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2022 Jun 6;15(6):721.

    The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123.  [Abstract]

    Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952.  [Abstract]

    Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15:96:468-479.  [Abstract]

    CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146.  [Abstract]

    MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.

    Sodium Channel アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Nav1.1 Nav1.2 Nav1.3 Nav1.4 Nav1.5 Nav1.6 Nav1.7 Nav1.8 Nav1.9

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1]. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment[2] (Ex/Em = 356/451 nm).

    体外実験

    Guidelines (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
    1. Preparation of DAPI dihydrochloride working solution
    Dilute the stock solution with serum-free cell culture medium or PBS to obtain a working solution of 1-10 μg/mL.
    Note: Please adjust the concentration of the DAPI dihydrochloride working solution according to your needs.
    2. Cell staining
    2.1 Suspension cells (96-well plate)
    a. Centrifuge at 1000 g at 4°C for 3-5 minutes, then discard the supernatant. Wash twice with PBS for 5 minutes each time. Adjust the cell density to 1×106/mL.
    b. Add 1 mL working solution and incubate at room temperature for 3-10 minutes.
    c. Centrifuge at 400 g at 4°C for 3-4 minutes and discard the supernatant.
    d. Wash twice with PBS for 5 minutes each time.
    e. Resuspend the cells in serum-free cell culture medium or PBS. Observe using a fluorescence microscope or flow cytometry.
    2.2 Adherent cells
    a. Culture adherent cells on sterile coverslips.
    b. Remove the coverslips from the culture medium and aspirate excess medium.
    c. Add 100 μL working solution, gently shake to fully cover the cells, and incubate at room temperature for 3-10 minutes.
    d. Wash twice with culture medium for 5 minutes each time. Observe using a fluorescence microscope or flow cytometer.

    Storage
    Store at -20°C for 1 year. Protect from light.

    Notes 1. Please adjust the concentration of the DAPI dihydrochloride working solution according to actual conditions.
    2. This product is for R&D use only and is not intended for use in drugs, household, or other purposes.
    3. For your safety and health, please wear a lab coat and disposable gloves during operation.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DAPI (dihydrochloride) 関連抗体
    分子量

    350.25

    分子式

    C16H17Cl2N5
    Emission (Em)

    451
    Excitation (Ex)

    356
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to green yellow

    SMILES

    N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 75 mg/mL (214.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5.56 mg/mL (15.87 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8551 mL 14.2755 mL 28.5510 mL
    5 mM 0.5710 mL 2.8551 mL 5.7102 mL
    10 mM 0.2855 mL 1.4276 mL 2.8551 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.90%

    COA (247 KB) HNMR (276 KB) RP-HPLC (261 KB) MS (217 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.8551 mL 14.2755 mL 28.5510 mL 71.3776 mL
    5 mM 0.5710 mL 2.8551 mL 5.7102 mL 14.2755 mL
    10 mM 0.2855 mL 1.4276 mL 2.8551 mL 7.1378 mL
    15 mM 0.1903 mL 0.9517 mL 1.9034 mL 4.7585 mL
    DMSO 20 mM 0.1428 mL 0.7138 mL 1.4276 mL 3.5689 mL
    25 mM 0.1142 mL 0.5710 mL 1.1420 mL 2.8551 mL
    30 mM 0.0952 mL 0.4759 mL 0.9517 mL 2.3793 mL
    40 mM 0.0714 mL 0.3569 mL 0.7138 mL 1.7844 mL
    50 mM 0.0571 mL 0.2855 mL 0.5710 mL 1.4276 mL
    60 mM 0.0476 mL 0.2379 mL 0.4759 mL 1.1896 mL
    80 mM 0.0357 mL 0.1784 mL 0.3569 mL 0.8922 mL
    100 mM 0.0286 mL 0.1428 mL 0.2855 mL 0.7138 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    DAPI dihydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DAPI28718-90-34',6-Diamidino-2-phenylindoleDNA StainSodium ChannelNa channelsNa+ channelsInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    DAPI dihydrochloride
    製品番号:
    HY-D0814
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.