Cat. No.: HY-N0410

Daucosterol

(Synonyms: Eleutheroside A; β-Sitosterol β-D-glucoside)

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Daucosterol is an orally active natural sterol compound, which has anti-inflammatory, anticancer and immunomodulatory activities. Daucosterol inhibits cancer cell proliferation by inducing autophagy through ROS-dependent manner. Daucosterol also inhibits colon cancer growth by inducing apoptosis, inhibiting cell migration and invasion and targeting caspase signalling pathway.

For research use only. We do not sell to patients.

Purity: 93.98%
CAS No.: 474-58-8
Formula: C₃₅H₆₀O₆
Molecular Weight:576.85

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Daucosterol Chemical Structure

CAS No.: 474-58-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human A2780 cells after 96 hrs by MTT assay Cytotoxicity against human A2780 cells after 96 hrs by MTT assay	[PMID: 17125236]
A549	ED₅₀	4.73 μg/mL Compound: beta-sitosterol-beta-D-glucopyranoside	Cytotoxicity against human A549 cells after 7 days Cytotoxicity against human A549 cells after 7 days	[PMID: 1453182]
A549	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human A549 cells after 96 hrs by MTT assay Cytotoxicity against human A549 cells after 96 hrs by MTT assay	[PMID: 17125236]
A549	GI₅₀	35.5 μM Compound: 6	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 20153184]
A549	IC₅₀	>20 μg/mL Compound: 10	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 22413887]
Bel-7402	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay	[PMID: 17125236]
BGC-823	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay	[PMID: 17125236]
Ca9-22	IC₅₀	>20 μg/mL Compound: 10	Cytotoxicity against human Ca9-22 cells by MTT assay Cytotoxicity against human Ca9-22 cells by MTT assay	[PMID: 22413887]
HCT-8	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay	[PMID: 17125236]
Hep 3B2	IC₅₀	>20 μg/mL Compound: 10	Cytotoxicity against human Hep3B cells by MTT assay Cytotoxicity against human Hep3B cells by MTT assay	[PMID: 22413887]
HepG2	IC₅₀	>20 μg/mL Compound: 10	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 22413887]
HL-60	GI₅₀	50.8 μM Compound: 6	Growth inhibition of human HL60 cells by MTT assay Growth inhibition of human HL60 cells by MTT assay	[PMID: 20153184]
HT-1080	ED₅₀	>100 μM Compound: 15	Antiproliferative activity against human HT1080 cells by MTT assay Antiproliferative activity against human HT1080 cells by MTT assay	[PMID: 11277741]
HT-29	GI₅₀	46.8 μM Compound: 6	Growth inhibition of human HT-29 cells by MTT assay Growth inhibition of human HT-29 cells by MTT assay	[PMID: 20153184]
HUVEC	ED₅₀	>5 μg/mL Compound: beta-sitosterol glucoside	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 15043409]
LNCaP	ED₅₀	>5 μg/mL Compound: beta-sitosterol glucoside	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15043409]
Lu1	ED₅₀	>5 μg/mL Compound: beta-sitosterol glucoside	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15043409]
MCF7	ED₅₀	44.39 μg/mL Compound: beta-sitosterol-beta-D-glucopyranoside	Cytotoxicity against human MCF7 cells after 7 days Cytotoxicity against human MCF7 cells after 7 days	[PMID: 1453182]
MCF7	ED₅₀	>5 μg/mL Compound: beta-sitosterol glucoside	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 15043409]
MCF7	IC₅₀	>20 μg/mL Compound: 10	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 22413887]
MDA-MB-231	IC₅₀	>20 μg/mL Compound: 10	Cytotoxicity against human MDA-MB-231 cells by MTT assay Cytotoxicity against human MDA-MB-231 cells by MTT assay	[PMID: 22413887]
NCI-H460	IC₅₀	>50 μM Compound: 3	Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay	[PMID: 28606759]
P388	ED₅₀	53 μg/mL Compound: Beta-sitosterol glycoside	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 3404159]
RAW264.7	IC₅₀	77.4 μM Compound: 116	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production	[PMID: 31255927]
SK-OV-3	GI₅₀	29.8 μM Compound: 6	Growth inhibition of human SKOV3 cells by MTT assay Growth inhibition of human SKOV3 cells by MTT assay	[PMID: 20153184]

In Vitro

Daucosterol (0.01-100 μM, 48 h) inhibits the proliferation of MCF-7, AGS, MGC803 and BGC823 cells in a dose-dependent manner, with IC₅₀s of 19.96 μM, 3.13 μM, 24.19 μM and 16.95 μM, respectively^[1].
Daucosterol (50 μM, 24 h) significantly increases ROS production in MCF-7 and BGC823 cells^[1].
Daucosterol (0-80 μM, 48 h) inhibits proliferation and promotes apoptosis in PC3 and LNCap cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

PC3 and LNCap cells
Concentration:

0-80 μM
Incubation Time:

48 h
Result:

Increased the expressions of cleaved caspase 3, cleaved caspase 9, Bax protein, LC3II/LC3I ration and Beclin 1.
Decreased the the expressions of Bax protein and p62.

In Vivo

Daucosterol (0.5-2.5 mg/kg, p.o., once per day for 7 days) significantly inhibits the H22 tumor growth in ICR mice inoculated with H22 hepatoma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 474-58-8
Appearance Solid
Molecular Weight 576.85
Formula C₃₅H₆₀O₆
Color White to off-white
SMILES

C[C@H](CC[C@@H](CC)C(C)C)[C@](CC1)([H])[C@]2(C)[C@]1([H])[C@]([C@]3([H])CC2)([H])CC=C([C@]3(C)CC4)C[C@H]4O[C@@]5([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O5
Synonyms

Eleutheroside A; β-Sitosterol β-D-glucoside
Structure Classification
- Steroids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

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Br J Pharmacol

Schedule-dependent neuroprotection by pioglitazone in a novel model of α-synucleinopathy in rats: Integrated behavioural and histological outcomes. [Abstract]

2026 Apr 25. PMID: 42033189
PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
Eur J Pharmacol

Daucosterol disturbs redox homeostasis and elicits oxidative-stress mediated apoptosis in A549 cells via targeting thioredoxin reductase by a p53 dependent mechanism. [Abstract]

2019 Jul 15:855:112-123. PMID: 31059712

Daucosterol purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2019 Jul 15:855:112-123. [Abstract]

Western blot analysis of p53 expression and phosphorylation in DS treated A549 cells with the indicated concentrations after 72 h incubation. DS up-regulates p53 expression and phosphorylation which resulted in activation of downstream regulator p21.
Ann Bot

Integrated LC/MS-MS approaches reveal toxicity differences across seed layers of Cycas angulata. [Abstract]

2026 Jun 17:mcag169. PMID: 42308138
Behav Brain Res

A single intranigral administration of β-sitosterol β-d-glucoside elicits bilateral sensorimotor and non-motor alterations in the rat. [Abstract]

2020 Jan 27;378:112279. PMID: 31606429
MethodsX

A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents. [Abstract]

2020 Feb 20;7:100821. PMID: 32195138
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

DMSO : 3.33 mg/mL (5.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7336 mL

8.6678 mL

17.3355 mL

5 mM

0.3467 mL

1.7336 mL

3.4671 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhao C, et al. Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner. Life Sci. 2015 Sep 15;137:37-43. [Content Brief]

[2]. Gao P, et al. Daucosterol induces autophagic-dependent apoptosis in prostate cancer via JNK activation. Biosci Trends. 2019 May 12;13(2):160-167. [Content Brief]

[3]. Jiang LH, et al. Daucosterol protects neurons against oxygen-glucose deprivation/reperfusion-mediated injury by activating IGF1 signaling pathway. J Steroid Biochem Mol Biol. 2015 Aug;152:45-52. [Content Brief]

[4]. Wang GQ, et al. Daucosterol inhibits colon cancer growth by inducing apoptosis, inhibiting cell migration and invasion and targeting caspase signalling pathway. Bangladesh Journal of Pharmacolog, 2016, 11(2): 395-401.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7336 mL	8.6678 mL	17.3355 mL	43.3388 mL
DMSO	5 mM	0.3467 mL	1.7336 mL	3.4671 mL	8.6678 mL

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Daucosterol

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Publications Citing Use of MedChemExpress (MCE) Daucosterol