2. Apoptosis
  3. Ferroptosis
  4. Erastin2

Erastin2, an Erastin (HY-15763) analog, is a ferroptosis inducer and a potent, selective inhibitor of the system xc(-) cystine/glutamate transporter

For research use only. We do not sell to patients.

CAS No. : 1695533-44-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Erastin2 Related Antibodies

Top Publications Citing Use of Products

    Erastin2 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Feb 17:e23198.

    Cancer cells were isolated from the resected primary tumor in 4T1‐Balb/c and M231‐NSG models and incubated with dose‐range RSL3 and Erastin2 for 48 h. Cell viability was assessed using an absorbance‐based assay (MTT), and results are expressed as a ratio to the untreated condition (n = 6–8 mice per group).

    Erastin2 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8096):477-486.  [Abstract]

    Cell viability of B16-F0 and LN metastatic lines treated with increasing concentrations of Erastin2 or 0.5 μM for 24 h.

    Erastin2 purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2025 Aug 18:e0047125.  [Abstract]

    Erastin2 (0.125-2 μM) inhibited the proliferation of P. falciparum as single agents, yet none of them exhibited synergy with Pyrimethamine.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Erastin2, an Erastin (HY-15763) analog, is a ferroptosis inducer and a potent, selective inhibitor of the system xc(-) cystine/glutamate transporter[1][2][3][4]

    In Vitro

    Erastin2 (2 μM, 48 h) induced ferroptosis correlates with acute amino acid deprivationinduced proliferative arrest in a manner that can be independent of mTOR inhibition and GCN2/ATF4 pathway activation in both U-2 OSN and HT-1080N cells in response to erastin2[1].
    Erastin2 (1 μM, 48 h) -induced HT-1080 TP53 KO fibrosarcoma cell death are sensitive regardless of nutlin-3 pretreatment [4].
    p53 sabilization suppresses Erastin2 (1 μM, 48 h) -induced ferroptosis in response to system xc(-) inhibition in HT-1080 TP53 KO fibrosarcoma cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Erastin2 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: HT-1080N fibrosarcoma cells
    Concentration: 1 or 2 μM
    Incubation Time: 48 h
    Result: Consistently initiated cell death 8 h earlier than Camptothecin (HY-16560), such that Camptothecin (HY-16560) may not have time to act before the induction of ferroptosis has already committed cells to death via this pathway in HT-1080N fibrosarcoma cells.
    The six most potent compounds (Fer-1, SKI II, phenothiazine, JNJ-26854165, AZD3463, and bazedoxifene) all suppressed Erastin2 and ML162-induced ferroptosis with sub-150 nM potency in HT-1080N fibrosarcoma cells.

    Cell Proliferation Assay[1]

    Cell Line: U-2 OSN and HT-1080N cells
    Concentration: 2 μM
    Incubation Time: 48 h
    Result: Deprivation of glutamine, lysine, valine, methionine, and arginine, all suppressed ferroptosis in both U-2 OSN and HT-1080N cells in response to erastin2.

    Cell Proliferation Assay[4]

    Cell Line: Human HT-1080 TP53 KO fibrosarcoma cells
    Concentration: 1 μM, pretreated for 48 h with or without Nutlin-3 (HY-50696)
    Incubation Time: 48 h
    Result: Control cells pretreated with nutlin-3 were less sensitive to erastin2-induced cell death, by contrast, TP53 KO cells were equally sensitive to erastin2 regardless of nutlin-3 pretreatment.
    Molecular Weight

    623.14

    Formula

    C36H35ClN4O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1C2=C(N=C(CN3CCN(CC3)C(COC4=CC=C(C=C4)Cl)=O)N1C5=C(OC(C)C)C=CC(C6=CC=CC=C6)=C5)C=CC=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (160.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6048 mL 8.0239 mL 16.0478 mL
    5 mM 0.3210 mL 1.6048 mL 3.2096 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 98.55%

    SDS (252 KB)

    COA (253 KB) HNMR (283 KB) LCMS (96 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6048 mL 8.0239 mL 16.0478 mL 40.1194 mL
    5 mM 0.3210 mL 1.6048 mL 3.2096 mL 8.0239 mL
    10 mM 0.1605 mL 0.8024 mL 1.6048 mL 4.0119 mL
    15 mM 0.1070 mL 0.5349 mL 1.0699 mL 2.6746 mL
    20 mM 0.0802 mL 0.4012 mL 0.8024 mL 2.0060 mL
    25 mM 0.0642 mL 0.3210 mL 0.6419 mL 1.6048 mL
    30 mM 0.0535 mL 0.2675 mL 0.5349 mL 1.3373 mL
    40 mM 0.0401 mL 0.2006 mL 0.4012 mL 1.0030 mL
    50 mM 0.0321 mL 0.1605 mL 0.3210 mL 0.8024 mL
    60 mM 0.0267 mL 0.1337 mL 0.2675 mL 0.6687 mL
    80 mM 0.0201 mL 0.1003 mL 0.2006 mL 0.5015 mL
    100 mM 0.0160 mL 0.0802 mL 0.1605 mL 0.4012 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Erastin2 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Erastin21695533-44-8Erastin 2Erastin-2FerroptosisErastin analogferroptosisxc(-) inhibitorInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Erastin2
    Cat. No.:
    HY-139087
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.