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  3. α-synuclein Environmental Pollutants
  4. Fast Green FCF

Fast Green FCF  (Synonyms: FD&C Green No. 3; Food green 3; C.I. 42053)

Art. -Nr.: HY-D0914 Reinheit: 99.95%
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Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function.

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Fast Green FCF

Fast Green FCF Chemische Struktur

CAS. Nr. : 2353-45-9

Größe Preis Verfügbarkeit Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 Auf Lager
Solution
10 mM * 1 mL in Water Auf Lager
Solid
500 mg Auf Lager
1 g Auf Lager
5 g   Angebot einholen  
10 g   Angebot einholen  

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

This product is a controlled substance and not for sale in your territory.

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Other Forms of Fast Green FCF:

Fast Green FCF Related Antibodies

Top Publications Citing Use of Products

Alle α-synuclein Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
α-synuclein α-synuclein Aggregation α-synuclein/CHMP2B

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Beschreibung

Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function[1][2][3][4][5][6][7][8][9].

IC50 & Target[1][2][3][4][5][6][7][8][9]

α-synuclein Aggregation

 

In Vitro

Fast Green FCF (molar ratios of HEWL/FCF: 1 to 0.01-1 to 1, 10 days) suppresses the generation of lysozyme fibrils in a concentration-dependent manner and exhibits disaggregating activity against the preformed/existing lysozyme fibrils[2].
Fast Green FCF (100 μg/mL-1 mg/mL) inhibits synaptic activity in rat hippocampal interneurons, with half-maximum effective concentration of ~200 μg/mL (250 μM)[3].
Fast Green FCF (5-100 μM, 24 h) inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity in PC12 cells[4].
Fast green FCF (molar ratios of Aβ40 to FGF of 3:1-1:3) alleviates the cytotoxicity induced by Aβ40 fibrillogenesis in PC12 cells[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fast Green FCF Related Antibodies

Cell Viability Assay[4]

Cell Line: PC12 cells with α-syn fibrils
Concentration: 5, 25, 50, 100 μM
Incubation Time: 24 h
Result: Reduced the cytotoxicity, and the cell viability respectively reached 66.2% (5 μM) and 72.7% (25 μM).
Increased cell viability, reaching 76.4% (50 μM) and 89.0% (100 μM), respectively.
In Vivo

Fast Green FCF (100 mg/kg, i.p., 30 min prior to each trial) attenuates Aβ-induced cognitive impairment in mice[5].
Fast Green FCF (10-100 mg/kg, i.p., once daily, 30 min before surgery) prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice[6].
Fast Green FCF (100 mg/kg, i.p., daily for 7 days) attenuates lipopolysaccharide-induced depressive-like behavior and downregulates TLR4/Myd88/NF-κB signal pathway in the mouse hippocampus[7].
Fast Green FCF (30-100 mg/kg, i.p., daily for 9 days) alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model[8].
Fast Green FCF (0.017-0.041 gm per kg body weight, p.o., 42 days) causes a significant decrease in testicular weight, tubular diameter, sperm density, and sperm motility[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Aβ-induced cognitive impairment mice[5]
Dosage: 30, 100 mg/kg
Administration: Intraperitoneal injection (i.p.), 30 min prior to each trial
Result: Did not alter the recognition index.
Prevented the Aβ42-induced recognition impairment at 100 mg/kg.
Prevented the Aβ42-induced impairment of spatial learning at 100 mg/kg.
reduced the Aβ42-induced memory impairment.
Molekulargewicht

808.85

Formel

C37H34N2Na2O10S3
CAS. Nr.
Appearance

Solid

Color

Dark green to black

Emission (Em)

522
Excitation (Ex)

486
SMILES

O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

H2O : 10 mg/mL (12.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2363 mL 6.1816 mL 12.3632 mL
5 mM 0.2473 mL 1.2363 mL 2.4726 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Reinheit & Dokumentation

Purity: 99.95%

SDS (644 KB)

COA (259 KB) HNMR (325 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2363 mL 6.1816 mL 12.3632 mL 30.9081 mL
5 mM 0.2473 mL 1.2363 mL 2.4726 mL 6.1816 mL
10 mM 0.1236 mL 0.6182 mL 1.2363 mL 3.0908 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Fast Green FCF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fast Green FCF2353-45-9FD&C Green No. 3 Food green 3 C.I. 42053Food green3Food green 3Food green-3α-synucleinEnvironmental Pollutantsα-SynEnvironmental Contaminantsprotein stainquantitative stainhistonescarcinogenicInhibitorinhibitorinhibit

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