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  3. Fluorescent Dye Reactive Oxygen Species (ROS)
  4. H2DCFDA

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm).

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CAS 番号 : 4091-99-0

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
5 mg $20 在庫あり
10 mg $25 在庫あり
50 mg $45 在庫あり
100 mg $60 在庫あり
250 mg $135 在庫あり
500 mg $265 在庫あり
1 g $525 在庫あり
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カスタマーレビュー

Based on 747 publication(s) in Google Scholar

Other Forms of H2DCFDA:

DCFH-DA 関連抗体

Top Publications Citing Use of Products

顧客検証

IF
Flow Cytometry
Cell Imaging/Staining

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030.  [Abstract]

    DCFH-DA (5 μM; 30 min), a specific indicator for ROS detection, was employed to evaluate overall ROS levels in 4T1 cells.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4251-4268.  [Abstract]

    Inside A375 melanoma cells, the ROS level is measured with the help of the DCFH-DA probe under CLSM imaging.
    Representative CLSM images of ROS generation inside A375 cells after different treatments with or without light irradiation (scale bar: 50 μm; blue: Hoechst 33342, green: DCFH-DA probe; Xe lamp, 650 nm, 6.4 mW/cm2, 12 min).

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4403-4421.  [Abstract]

    Flow cytometry analysis of ROS levels in HQMitofactories using the DCFH-DA (10 μM; 30 min) probe. HQ-Mitofactories were exposed to 400 μM H2O2 for 6 h prior to measurement.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4403-4421.  [Abstract]

    DCFH-DA (10 μM; 30 min). Fluorescent images of ROS levels in PC12 cells. ROS were labeled with the DCFH-DA probe (green) and nuclei were stained with Hoechst 33342 (blue). Scale bar, 40 μm. Data are presented as mean ± SD.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4403-4421.  [Abstract]

    Confocal fluorescent images of ROS levels in HQ-Mitofactories. ROS were labeled with the DCFH-DA (10 μM; 30 min) probe (green) and nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 μm.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Nov 26:e2409176.  [Abstract]

    The ROS levels in the oxygen-glucose deprivation/reoxygenation (OGD/R) model of HT22 cells were detected using DCFH-DA (10 µm; 20 min). Confocal laser scanning microscope (CLSM) images showed bright green fluorescence in the OGD/R group, indicating a significant increase in ROS.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Oct 12:e2411618.  [Abstract]

    The capability of different biocatalysts to eliminate ROS was assessed through representative images of DCFH-DA (30 min) fluorescence across various cell types (MSCs, RSC96, Raw264.7) subjected to distinct treatments.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2024 Jul 9:41:30-45.  [Abstract]

    DCFH-DA (20 min) assay was further examined to evaluate the ROS scavenging capacity on the cellular level. As ROS oxidizes DCFH into DCF, it emits green fluorescence, which can be used to estimate the remaining ROS.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2024 May 23.

    DCFH-DA (10 µM; 20 min). Fluorescence images of DCFH illustrating intracellular ROS in BMSCs after different treatment (scale bar: 200 µm).

    H2DCFDA purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Sep;13(9):e12505.  [Abstract]

    H2DCFDA (10 µM; 20 min). Detection of intracellular ROS generation in A549 cells exposed to 20 µM H2O2. Scale bar: 200 µm.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2023 Jan;13(1):113-127.

    Treated HL-1 cells were washed three times and incubated with DMEM medium containing H2DCFDA (10 μM; 30 min) at 37 °C. The cells then were washed three times with warm DMEM medium and intracellular ROS production was detected by flow cytometry.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May;9(13):e2103317.  [Abstract]

    CLSM images of HUVEC cells using DCFH‐DA (0.5 hours) as the fluorescent probe: Control, H2O2, H2O2+10 µg/mL1 PDA NPs, H2O2+40 µg/mL PDA NPs, H2O2+80 µg/mL PDA NPs.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Nov:290:121842.  [Abstract]

    DCFH-DA (10 μM; 30 min) or C11-BODIPY-labeled cells were substantially increased after treatment with 1 μM FeSO4.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 May;284:121513.  [Abstract]

    DCFH-DA (10 μM; 30 min). The ROS generation in 4T1 cells incubated with various formulations for 4 h observed by CLSM.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Jul 21;287:121688.  [Abstract]

    CLSM images of 4T1 tumor cells stained with DCFH-DA (15 min) after different treatments.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2022 Oct 5;439:129502.  [Abstract]

    Representative DCFH-DA (10 μM; 30 min) staining images (Green) in PBNs exposed PSNPs and/or LPS in vivo and in vitro.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Cell. 2021 Jun 24;184(13):3528-3541.e12.  [Abstract]

    Stained protoplasts with H2DCFDA for the detection of ROS production and PI for PM integrity and tracked the process by live-cell imaging.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2021 Oct 26;15(10):16286-16297.  [Abstract]

    CLSM image of 4T1 cells after being treated with MM, MMP, and MMTP; cells are stained with DCFH-DA (20 μM; for 1 h).

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2021 Oct;277:121103.  [Abstract]

    In vitro experiments are conducted to measure the cellular ROS levels in A549 cells from different treatments via flow cytometry using fluorescent probe H2DCFDA (5 μM; 30 min) to better understand the potential molecular mechanism of SOD@ARA290-HBc on radiation-induced lung injury. H2DCFDA fluorescence intensity in A549 cells significantly increased after irradiation.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Small. 2021 Aug;17(32):e2101368.  [Abstract]

    Laser scanning confocal fluorescence microscopy images of HT-1080 cells treated with different nanodrugs for the detection of intracellular lipid peroxidation and total ROS using C11-BODIPY and DCFH-DA (100 nM; 10 min) as the corresponding fluorescence probes.

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Curr Biol. 2021 Jul 26;31(14):3004-3016.e4.  [Abstract]

    Co-staining stigmas with H2DCFDA (50 μM; for 1-2 hours) and the cell wall indicator propidium iodide (PI) shows that ROS is located in the cytoplasm of papilla cells, near the periphery of the plasma membrane.

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    製品説明

    H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)[1].

    体外実験

    Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
    1. Prepare the storage solution and working solution 1.1 Prepare a 10 mM stock solution of H2DCFDA using anhydrous DMSO.
    1.2 Dilute the stored solution with pre-warmed serum-free cell culture medium or PBS to prepare a working solution of 1-10 μM H2DCFDA.
    Note: Please adjust the concentration of the H2DCFDA working solution according to the actual situation, and prepare it as needed.
    2. Cell Staining (Suspension Cells) 2.1 Centrifuge the cells and add PBS for two washes, each for 5 minutes. The cell density is 1×106 cells/mL.
    2.2 Add 1 mL of the dye working solution and incubate at room temperature for 5 to 30 minutes.
    2.3 400 grams, centrifuge for 3-4 minutes, then discard the supernatant.
    2.4 Add PBS to wash the cells twice, for 5 minutes each time.
    2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
    3. Cell Staining (Monolayer Cells) 3.1 Cultivate adherent cells on sterile coverslips.
    3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
    3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
    3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Then observe using a fluorescence microscope or flow cytometer.
    Note: If flow cytometry is to be used for detection, the cells should first be digested with trypsin, resuspended, and then stained.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DCFH-DA 関連抗体
    分子量

    487.29

    分子式

    C24H16Cl2O7
    Emission (Em)

    517
    Excitation (Ex)

    492
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=C(Cl)C(OC(C)=O)=C4)C=C(OC(C)=O)C(Cl)=C3
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 250 mg/mL (513.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0522 mL 10.2608 mL 20.5217 mL
    5 mM 0.4104 mL 2.0522 mL 4.1043 mL
    10 mM 0.2052 mL 1.0261 mL 2.0522 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション

    純度: 99.82%

    COA (252 KB) HNMR (228 KB) LCMS (120 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献
    キナーゼ実験

    ROS Measurements[1]
    For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0522 mL 10.2608 mL 20.5217 mL 51.3042 mL
    5 mM 0.4104 mL 2.0522 mL 4.1043 mL 10.2608 mL
    10 mM 0.2052 mL 1.0261 mL 2.0522 mL 5.1304 mL
    15 mM 0.1368 mL 0.6841 mL 1.3681 mL 3.4203 mL
    20 mM 0.1026 mL 0.5130 mL 1.0261 mL 2.5652 mL
    25 mM 0.0821 mL 0.4104 mL 0.8209 mL 2.0522 mL
    30 mM 0.0684 mL 0.3420 mL 0.6841 mL 1.7101 mL
    40 mM 0.0513 mL 0.2565 mL 0.5130 mL 1.2826 mL
    50 mM 0.0410 mL 0.2052 mL 0.4104 mL 1.0261 mL
    60 mM 0.0342 mL 0.1710 mL 0.3420 mL 0.8551 mL
    80 mM 0.0257 mL 0.1283 mL 0.2565 mL 0.6413 mL
    100 mM 0.0205 mL 0.1026 mL 0.2052 mL 0.5130 mL
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    H2DCFDA Related Classifications

    • Molarity Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    H2DCFDA4091-99-0DCFH-DA 2',7'-Dichlorodihydrofluorescein diacetateFluorescent DyeReactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

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    製品名:
    H2DCFDA
    製品番号:
    HY-D0940
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