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HPPH 

Cat. No.: HY-13722 Purity: >98.0%
Handling Instructions

HPPH is a second generation photosensitizer, which acts as a photodynamic therapy (PDT) agent.

For research use only. We do not sell to patients.

HPPH Chemical Structure

HPPH Chemical Structure

CAS No. : 149402-51-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 252 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
Estimated Time of Arrival: December 31
10 mg USD 320 In-stock
Estimated Time of Arrival: December 31
25 mg USD 550 In-stock
Estimated Time of Arrival: December 31
50 mg USD 800 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1350 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

HPPH is a second generation photosensitizer, which acts as a photodynamic therapy (PDT) agent.

In Vitro

Fluorescence image of 4T1 cells incubated with 0.49 µg/mL GO-PEG, 1 μM HPPH (free HPPH) or equivalent amount of GO-PEG-HPPH (1 µM HPPH and 0.49 µg/mL GO-PEG) after 24 h. The cellular uptake of GO-PEG-HPPH and HPPH is investigated with 4T1 murine mammary cancer cells. The cells are incubated with GO-PEG-HPPH and free HPPH at equivalent HPPH concentration (1 µM) for 24 h and then observed with a confocal microscope. Cells treated with GO-PEG-HHPH shows stronger fluorescence signal than those treated with free HPPH. In fact, the fluorescence of HPPH is rather weak[1].

In Vivo

Tumors are treated with an immune-enhancing PDT regimen followed by a tumor-controlling PDT regimen can leads to enhancement of anti-tumor immunity, while retaining effective control of primary tumor growth. To test this hypothesis, a combination treatment regimen is devised in which Colo26-HA tumor-bearing BALB/c mice are treated with a HPPH-PDT regimen known to lead to enhanced anti-tumor immunity (0.4 μmoles/kg HPPH followed 18 h later by illumination with 665 nm light for a total dose of 48 J/cm2). Following illumination, mice are rested for 9 days; on the ninth day, mice are injected with HPPH. On day 10 following the first treatment, tumors are treated with a tumor control treatment regimen (illumination with 665 nm light for a total dose of 132 J/cm2 given)[2].

Clinical Trial
Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5703 mL 7.8515 mL 15.7030 mL
5 mM 0.3141 mL 1.5703 mL 3.1406 mL
10 mM 0.1570 mL 0.7852 mL 1.5703 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

4T1 cells are cultured in 96-well cell culture plates at 1×104/well for 24 h and then treated with GO-PEG-HPPH, HPPH, or GO-PEG at a series of concentrations (0.078125, 0.15625, 0.3125, 0.625, 1.25, 2.5, 5, 10, and 20 μM). Then, 20 µL of MTT solution (5.0 mg/mL) is added to each well. After the 4 h incubation with the MTT, the media are removed and 100 µL of DMSO is added to solubilize the formazan crystals. The cell toxicity efficacy is measured with a microplate reader at an absorbance of 570 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
Tumor-bearing mice are injected in the tail vein with 0.4 μmol/kg HPPH or 5 mg/kg Porfimer sodium (PII), followed 18-24 h later by illumination to a total light dose of 48 J/cm2 or 132 J/cm2 delivered at a light dose-rate of 14 mW/cm2. Control mice are treated with photosensitizer or light alone. Mice receiving a combination PDT regimen are treated initially with 0.4 μmol/kg HPPH or 5 mg/kg PII followed 18-24 h later by light dose of 48 J/cm2 given at 14 mW/cm2; 9 days later, mice are again injected with photosensitizer and tumors are illuminated with light at a dose of 132 J/cm2 given at 14 mW/cm2[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

636.82

Formula

C₃₉H₄₈N₄O₄

CAS No.

149402-51-7

SMILES

OC(CC[[email protected]@H]([[email protected]@H]1C)/C2=C(C3)/C4=C(C(C)=C(/C=C5C(CC)=C(C)C(/C=C6N/C(C(C)=C\6C(C)OCCCCCC)=C\C1=N2)=N/5)N4)C3=O)=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
HPPH
Cat. No.:
HY-13722
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HPPH

Cat. No.: HY-13722