2. Autophagy Apoptosis
  3. Autophagy Mitophagy Ferroptosis
  4. Hemin

ヘミン  (Synonyms: Hemin; Hemin chloride)

製品番号: HY-19424 純度: 99.59%
COA 取扱説明書 Technical Support

Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

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CAS 番号 : 16009-13-5

容量 価格（税別） 在庫状況 数量
1 g $50 在庫あり
5 g $90 在庫あり
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カスタマーレビュー

Based on 85 publication(s) in Google Scholar

ヘミン 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
In Vivo Efficacy Study
IHC
IF

    Hemin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(22):e2206798.  [Abstract]

    Hemin (dissolved in DMSO to the required concentration and stirred at RT for 2 h.) induced ferroptosis more rapidly when treated in combination with donafenib+GSK-J4.

    Hemin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Apr;19(4):516-526.  [Abstract]

    MCA205 and B16 cells treated with 20 μM Hemin or vehicle were infected with HSV-1 (0.25 MOI), and the expression level of BACH1 and genome copies of HSV-1 were measured by western blotting and qRT–PCR, respectively, at 24 hpi.

    Hemin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Apr;19(4):516-526.  [Abstract]

    Hemin (50 mg/kg hemin daily by intraperitoneal injection) treatment further enhanced CD45+CD8+ T lymphocyte infiltration in MCA205 fibrosarcoma tumors infected with HSV-1.

    Hemin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Apr;19(4):516-526.  [Abstract]

    Hemin (50 mg/kg hemin daily by intraperitoneal injection) treatment further enhanced CD45+CD8+ T lymphocyte infiltration in MCA205 fibrosarcoma tumors infected with HSV-1.

    Hemin purchased from MedChemExpress. Usage Cited in: Genome Biol. 2022 Dec 15;23(1):259.  [Abstract]

    Hemin (0, 20, 40, 80 µM; 2 h) increases G4 levels in a dose-dependent manner in HeLa cells. Immunostaining of G4 using BG4-EGFP (green). The nuclei are stained with DAPI (blue).

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    製品説明

    Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

    IC50 & Target

    Heme oxygenase[1]
    体外実験

    Hemin and PGJ2, used as positive controls, strongly increase both expression and activity of HMOX after 4 and 12 h, respectively. Indeed, a significant effect is found of 30 μM Hemin on cell proliferation in all used cell lines after 48 h, which is dose-dependent. Hemin treatment decreases cell proliferation to 62±5 %, 51±3 %, and 38±8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively, with p<0.0001 for all comparisons. Furthermore, enhancement of anti-proliferative effects of statins is observed by Hemin, documented as decreased cell proliferation after 48 h of co-treatment[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ヘミン 関連抗体
    体内実験

    Following the i.p. administration of Hemin (100 μmol/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    651.94

    分子式

    C34H32ClFeN4O4
    CAS 番号
    Appearance

    Solid

    Color

    Dark purple to black

    SMILES

    [Cl-][Fe+3]123[N-]4C5=C(CCC(O)=O)C(C)=C4C=C(C(C=C)=C6C)[N]1=C6C=C(C(C=C)=C7C)[N-]2C7=CC(C(C)=C8CCC(O)=O)=[N]3C8=C5
    Structure Classification
    Initial Source

    Red blood cells
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    1M NaOH : 6.67 mg/mL (10.23 mM; ultrasonic and adjust pH to 12 with NaOH)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5339 mL 7.6694 mL 15.3388 mL
    5 mM 0.3068 mL 1.5339 mL 3.0678 mL
    10 mM 0.1534 mL 0.7669 mL 1.5339 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.59%

    COA (260 KB) RP-HPLC (246 KB) LCMS (94 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [1]

    For the cell proliferation assay, cells are seeded into 96 well (5-12.5×104 cells per mL according to the cell line) and kept at 37°C and 5 % CO2. After 24 h, cells are treated with statins or/and Hemin, followed by the MTT test as a general cell proliferation assay[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [2]

    Mice[2]
    Eight- to ten-week-old male BABL/c mice are used for the ischemia-reperfusion (I/R) experiments. The animals are divided into five groups as follows: (1) the sham group undergo isolation of the bilateral renal arteries without clamping; (2) the vehicle group receive an intraperitoneal (i.p.) injection of 4 mL/kg PBS as a vehicle control (with IRI); (3) the Hemin-preconditioned group receive Hemin, a potent inducer of HO-1, at 100 μmol/kg i.p.; (4) the Hemin plus ZnPP group receive zinc protoporphyrin IX, an inhibitor of HO-1 activity, at 5 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p.; and (5) the Hemin plus PD98059 group receive PD98059, an inhibitor of ERK1/2 activity, at 10 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p. Both inhibitors are administered i.p. 2 h before I/R, whereas Hemin was administered 8 h before I/R.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    1M NaOH 1 mM 1.5339 mL 7.6694 mL 15.3388 mL 38.3471 mL
    5 mM 0.3068 mL 1.5339 mL 3.0678 mL 7.6694 mL
    10 mM 0.1534 mL 0.7669 mL 1.5339 mL 3.8347 mL
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    Hemin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hemin16009-13-5AutophagyMitophagyFerroptosisMitochondrial AutophagyInhibitorinhibitorinhibit

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