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  4. Hexadimethrine bromide

Hexadimethrine bromide  (Synonyms: Polybrene; 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide)

Cat. No.: HY-112735
COA
SDS
Instrucciones de manejo Technical Support

Hexadimethrine bromide (Polybrene) is a cationic polymer used routinely to enhance the efficiency of retrovirus vector-mediated gene transfer.

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Hexadimethrine bromide

Hexadimethrine bromide Estructura química

No. CAS : 28728-55-4

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Revisión del cliente

Based on 109 publication(s) in Google Scholar

Hexadimethrine bromide Related Antibodies

Top Publications Citing Use of Products

109 Publications Citing Use of MCE Hexadimethrine bromide

  • Actividad biológica

  • Protocolo

  • Pureza y Documentación

  • Referencias

  • Revisión del cliente

Descripciòn

Hexadimethrine bromide (Polybrene) is a cationic polymer used routinely to enhance the efficiency of retrovirus vector-mediated gene transfer[1][2].

In Vitro

Hexadimethrine bromide inhibits human mesenchymal stem cell proliferation during lentiviral transduction. Hexadimethrine bromide is considered non-toxic at low concentrations, but has been found to negatively affect cell proliferation in some cell types at concentrations greater than 10 μg/mL. Trypsinized cells exposed to Hexadimethrine bromide are visibly larger in size when viewed under the microscope[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hexadimethrine bromide Related Antibodies
Fòrmula

(C13H30N2Br2)n.
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

C[N+](CCCCCCC)(C)CCC[N+](C)(C)C.[Br-].[Br-].[n]
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvente y solubilidad
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

DMSO : 5 mg/mL (Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación

Purity: 99.69%

SDS (393 KB)

COA (240 KB) HNMR (234 KB)

Instrucciones de manejo (2659 KB)
Referencias
Ensayo celular
[1]

Trypsinized cells are washed and resuspended in medium at 2×104 cells/mL with or without rhFGF-2 (final concentration=10 ng/mL, PeproTech) and with or without Hexadimethrine bromide at a final Hexadimethrine bromide concentration of 1, 4, or 8 µg/mL. The different conditions are seeded in 96-well plates at 1×103 cells in 50 µL per well in triplicate and cultured at 37°C, 5% CO2. After 6, 9, or 24 hr, the medium is changed and subsequent medium changes occurred every 3-4 days with 50 µL of ± FGF-2 medium (10 ng/mL). Plates are harvested on day 4, 7, 14, and 21 by removing the medium and placing the plates in the -80°C freezer until the day of analysis. The CyQUANT assay is then performed on the wells[1].

MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Referencias
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Hexadimethrine bromide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hexadimethrine28728-55-4Polybrene 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromideBiochemical Assay ReagentsInhibitorinhibitorinhibit

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Nombre del producto:
Hexadimethrine bromide
Cat. No.:
HY-112735
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SAFETY DATA SHEET (SDS)

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