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  3. Lysozyme from chicken egg white

Lysozyme from chicken egg white is a bactericidal enzyme, and it lyses gram-positive bacteria. Lysozyme from chicken egg white can also be used for the research of HIV infection and pulmonary emphysema.

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No. CAS : 12650-88-3

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Revisión del cliente

Based on 10 publication(s) in Google Scholar

Other Forms of Lysozyme from chicken egg white:

Top Publications Citing Use of Products

    Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.

    Amount of RNA in ng per 106 cells from 5 mL N. europaea cultures extracted with NucleoSpin RNA XS kit preceded by enzymatic digestion with lysozyme (1-3 mg/mL) solution. The effect on RNA yield and quality of different lysozyme concentrations, different volumes of RA1 buffer after lysozyme digestion and different lysozyme incubation times are shown.

    Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.

    Examples of electropherograms generated from RNA samples with the Agilent BioAnalyzer 2100 represents an RNA sample extracted with the NS XS kit after pretreatment with a 1 mg/mL lysozyme solution and 15 min of incubation at 37°C with a RIN of 10.0.

    Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.

    The protocol on N. winogradskyi cultures using higher lysozyme concentrations, with different incubation times with 1 mg/mL lysozyme solution.

    Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.

    The protocol on N. winogradskyi cultures using higher lysozyme concentrations, with the addition of Proteinase K (ProtK) to a 15 mg/mL lysozyme solution and/or vortexing (indicated by V).

    Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.

    The protocol on N. winogradskyi cultures using various lysozyme concentrations during shaking incubation at 1,400 rpm for 15 min.

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    Descripciòn

    Lysozyme from chicken egg white is a bactericidal enzyme, and it lyses gram-positive bacteria. Lysozyme from chicken egg white can also be used for the research of HIV infection and pulmonary emphysema[1][2][3].

    IC50 & Target

    Bacteria[1]

    In Vitro

    Lysozyme is an ubiquitous enzyme. The hen egg is the most abundant source of Lysozyme, which constitutes approximately 3.4% of the albumen proteins. Lysozyme is a natural antimicrobial that hydrolyzes the β(1-4) glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine found in the peptidoglycan layer of the bacterial cell wall and causing cell lysis. The bactericidal effect of Lysozyme is primarily limited to gram-positive bacteria, including pathogens such as Listeria monocytogenes and certain Clostridium species as well as some spoilage organisms, including thermophilic spore-forming bacteria and certain yeasts. The gram-negative bacteria are more resistant to Lysozyme action because of their complex cell wall structure[1].
    Lysozyme (1 mg/mL) impairs the ability of hyaluronan (HA) to prevent elastase injury to elastic fibers[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Syrian hamsters exposes to aerosolized Lysozyme (20 mg in 20 ml of water; 50 min) prior to elastase administration showes significantly increased airspace enlargement[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    No. CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [Lysozyme(chicken egg white)]

    Structure Classification
    Initial Source

    chicken egg white

    Envío

    Room temperature in continental US; may vary elsewhere.

    Almacenamiento

    Please store the product under the recommended conditions in the Certificate of Analysis.

    Solvente y solubilidad
    In Vitro: 

    H2O : 10 mg/mL (Need ultrasonic and warming)

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureza y Documentación

    Referencias
    Ensayo de quinasas
    [1]

    For measurement of lytic activity in egg white at each pH, temperature, and CO2 condition, eggs are randomly selected from a flat of eggs (2 dozen eggs) obtained from a local grocery store. To determine the amount of egg white to be added to obtain a 0.001% lysozyme concentration, it is documented that chicken egg white contains approximately 3.4% lysozyme. For determining egg white activity, 0.030 g of albumen was added to 100 mL of the buffered solutions. This equated to a concentration of approximately 0.001% lysozyme. In addition, the egg white contains other antimicrobial proteins that are naturally present, as mentioned in the Introduction section[1].

    MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.

    Referencias
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Nombre del producto:
    Lysozyme from chicken egg white
    Cat. No.:
    HY-B2237
    Cantidad:
    MCE Japan Authorized Agent: