ML335
Based on 2 publication(s) in Google Scholar
ML335 is a selective activator of both TREK-1 and TREK-2.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.73%
- CAS. Nr.: 825658-06-8
- Formel: C15H14Cl2N2O3S
- Molecular Weight:373.25
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) ML335
More
Biologische Aktivität
TREK-1, TREK-2
Xenopus oocyte two-electrode voltage-clamp measurements show that ML335 and ML402 activate K2P2.1 and K2P10.1 but not K2P4.1 (14.3±2.7 μM, K2P2.1-ML335; 13.7±7.0 μM, K2P2.1-ML402; 5.2±0.5 μM, K2P10.1-ML335; and 5.9±1.6 μM, K2P10.1-ML402). Swapping the Lys271 equivalent between K2P2.1 and K2P4.1 results in a clear phenotype reversal for ML335 and M402 activation. ML335 and ML402 activate K2P2.1 in HEK293 cells similar to their effects in Xenopus oocytes (5.2±0.8 μM and 5.9±1.6 μM for ML335 and ML402, respectively (n≥3))[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 825658-06-8
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Appearance Solid
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Molecular Weight 373.25
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Formel C15H14Cl2N2O3S
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Color White to off-white
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SMILES
O=C(NCC1=CC=C(Cl)C=C1Cl)C2=CC=C(NS(=O)(C)=O)C=C2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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J Transl Med
ML335 inhibits TWIK2 channel-mediated potassium efflux and attenuates mitochondrial damage in MSU crystal-induced inflammation. [Abstract]2024 Aug 22;22(1):785. PMID: 39175013 -
Sci Rep
Calcium-dependent activation of TREK-1 and TREK-2 background potassium channels by calcineurin. [Abstract]2025 Dec 29. PMID: 41457157
Lösungsmittel & Löslichkeit
DMSO : 155 mg/mL (415.27 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
Mouse K2P2.1, human K2P4.1, and mutants are expressed from a previously described pIRES2-EGFP vector in HEK293T cells (ATTC). 70% confluent cells are transfected (in 35-mm diameter wells) with LipofectAMINE 2000 for 6 h, and plated onto coverslips coated with Matrigel. Effects of ML335, ML402 and arachidonic acid on K2P2.1 current at 0 mV are measured by whole-cell patch-clamp experiments 24 h after transfection. Acquisition and analysis are performed using pCLAMP9 and an Axopatch 200B amplifier. Pipette resistance ranges from 1 to 1.5 MΩ. Pipette solution contains the following: 145 mM KCl, 3 mM MgCl2, 5 mM EGTA and 20 mM HEPES (pH 7.2 with KOH). Bath solution contains the following: 145 mM NaCl, 5 mM KCl, 1 mM CaCl2, 3 mM MgCl2 and 20 mM HEPES (pH 7.4 with NaOH). K2P2.1 currents are elicited by a 1 s ramp from -100 to +50 mV from a -80 mV holding potential. After stabilization of the basal current, ML335 and ML402 are perfused at 200 mL per hour until potentiation is stably reached[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6792 mL | 13.3958 mL | 26.7917 mL | 66.9792 mL |
| 5 mM | 0.5358 mL | 2.6792 mL | 5.3583 mL | 13.3958 mL | |
| 10 mM | 0.2679 mL | 1.3396 mL | 2.6792 mL | 6.6979 mL | |
| 15 mM | 0.1786 mL | 0.8931 mL | 1.7861 mL | 4.4653 mL | |
| 20 mM | 0.1340 mL | 0.6698 mL | 1.3396 mL | 3.3490 mL | |
| 25 mM | 0.1072 mL | 0.5358 mL | 1.0717 mL | 2.6792 mL | |
| 30 mM | 0.0893 mL | 0.4465 mL | 0.8931 mL | 2.2326 mL | |
| 40 mM | 0.0670 mL | 0.3349 mL | 0.6698 mL | 1.6745 mL | |
| 50 mM | 0.0536 mL | 0.2679 mL | 0.5358 mL | 1.3396 mL | |
| 60 mM | 0.0447 mL | 0.2233 mL | 0.4465 mL | 1.1163 mL | |
| 80 mM | 0.0335 mL | 0.1674 mL | 0.3349 mL | 0.8372 mL | |
| 100 mM | 0.0268 mL | 0.1340 mL | 0.2679 mL | 0.6698 mL |
ML335 Related Classifications
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