1. Anti-infection
  2. Fungal

Myclobutanil 

Cat. No.: HY-B2148 Purity: 99.61%
Handling Instructions

Myclobutanil is a conazole class fungicide widely used as an agrichemical.

For research use only. We do not sell to patients.

Myclobutanil Chemical Structure

Myclobutanil Chemical Structure

CAS No. : 88671-89-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
100 mg USD 72 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Myclobutanil is a conazole class fungicide widely used as an agrichemical.

In Vitro

Myclobutanil reduces cell viability to <50% at 100 ppm and to <10% at 500 ppm. Myclobutanil promotes a slight, but significant, increase in fatty acid (FA)-induced steatotosis at doses from 1 to 100 ppm. Anti-apoptotic biomarkers are significantly reduced by Myclobutanil[1].

Solvent & Solubility
In Vitro: 

DMSO : 103 mg/mL (356.67 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4628 mL 17.3142 mL 34.6284 mL
5 mM 0.6926 mL 3.4628 mL 6.9257 mL
10 mM 0.3463 mL 1.7314 mL 3.4628 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

To further evaluate apoptosis, cell extracts are collected after 24 h of exposure to Myclobutanil, centrifuged, and analyzed with a multiplex biometric ELISA-based immunoassay containing dyed microspheres conjugated to a monoclonal antibody specific for the target protein. Apoptosis biomarkers are BCL-xL/Bak dimer and Mcl-1/Bak dimer, quantified using RBM Apoptosis Panel 3. Each experiment is performed in triplicate and apoptosis biomarker levels determined using the Bio-Plex Array Reader. The analytic concentrations are calculated using a standard curve, according to the manufacturer’s instructions[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

The hepatoma cell line HepG2 is used in this study. The cells are grown on tissue culture plates in an incubator with a humidified atmosphere (95% air/5% CO2 v/v) at 37°C. Steatosis is induced by incubating the hepatocytes with 6 mM of a 1:1 v/v mixture of oleic (18:1) and linoleic (18:2) fatty acids (Fas) for 24 h. After a wash with PBS, cells are exposed for an additional 24 h to Myclobutanil at 0.1, 1, 10, 100 or 500 ppm. Cytotoxicity is assessed in HepG2 cells (1.0×105 cells/well in 24-well plates) by measuring the reduction of the tetrazolium dye 3-(4, 5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTT)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

288.78

Formula

C₁₅H₁₇ClN₄

CAS No.

88671-89-0

SMILES

N#CC(C1=CC=C(Cl)C=C1)(CCCC)CN2N=CN=C2

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Myclobutanil
Cat. No.:
HY-B2148
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Myclobutanil

Cat. No.: HY-B2148