2. Membrane Transporter/Ion Channel
  3. Chloride Channel
  4. NPPB

NPPB is a blocker of the outwardly rectifying chloride channel (ORCC).

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NPPB

NPPB 構造式

CAS 番号 : 107254-86-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 41 在庫あり
Solution
10 mM * 1 mL in DMSO USD 41 在庫あり
Solid
5 mg $38 在庫あり
10 mg $60 在庫あり
25 mg $114 在庫あり
50 mg $192 在庫あり
100 mg $336 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 4 publication(s) in Google Scholar

Other Forms of NPPB:

NPPB 関連抗体

Top Publications Citing Use of Products

  • 生物活性

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製品説明

NPPB is a blocker of the outwardly rectifying chloride channel (ORCC).

IC50 & Target

ORCC[1]
Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
0.32 3
Compound: NPPB
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by FLIPR analysis
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by FLIPR analysis
[PMID: 30878828]
HEK293 EC50
0.32 3
Compound: NPPB
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by FLIPR analysis
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by FLIPR analysis
[PMID: 30878828]
HEK293 EC50
0.6 3
Compound: NPPB
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
[PMID: 30878828]
HEK293 EC50
0.6 3
Compound: NPPB
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
[PMID: 30878828]
Oocyte IC50
240 3
Compound: 5-Nitro-2-(3-phenylpropylamino)benzoate
TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes
TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes
[PMID: 11101640]
HEK293 EC50
0.32 3
Compound: NPPB
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by FLIPR analysis
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by FLIPR analysis
[PMID: 30878828]
HEK293 EC50
0.6 3
Compound: NPPB
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
[PMID: 30878828]
Oocyte IC50
240 3
Compound: 5-Nitro-2-(3-phenylpropylamino)benzoate
TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes
TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes
[PMID: 11101640]
体外実験

0.1 mM NPPB in the bath solution reduces channel open probability from 0.89±0.06 to 0.11±0.04 (n=5, P<0.01)[1]. Dose-dependent inhibition of chloride currents is observed with a 50% inhibitory concentration (IC50) of 125 μM NPPB. NPPB itself also shows cytotoxicity against glioma cells with a GI50 of approximately 500 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NPPB 関連抗体
分子量

300.31

分子式

C16H16N2O4
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)C1=CC([N+]([O-])=O)=CC=C1NCCCC2=CC=CC=C2
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (332.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3299 mL 16.6495 mL 33.2989 mL
5 mM 0.6660 mL 3.3299 mL 6.6598 mL
10 mM 0.3330 mL 1.6649 mL 3.3299 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.78%

COA (247 KB) HNMR (155 KB) LCMS (89 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[2]

Cells are seeded in the 96-well microtiter plate at a density of 5×103 cells per well and incubated at 37°C for 24 h in a humidified 5% CO2 atmosphere. After removing the culture medium, fresh media containing various concentrations of NPPB is added, and incubated for 24 h. Next, 100 μL of Thiazolyl blue tetrazolium bromide at 0.5 mg/mL is added to each well and incubated at 37°C for 1 h. Cells are then dissolved in 100 μL of DMSO, and the absorbance is measured at 570 nm with a Microplate Reader. Concentration-response curves of NPPB are fitted to a Hill equation to obtain GI50 and GI80 (50% and 80% growth inhibition concentrations, respectively) values[2].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3299 mL 16.6495 mL 33.2989 mL 83.2473 mL
5 mM 0.6660 mL 3.3299 mL 6.6598 mL 16.6495 mL
10 mM 0.3330 mL 1.6649 mL 3.3299 mL 8.3247 mL
15 mM 0.2220 mL 1.1100 mL 2.2199 mL 5.5498 mL
20 mM 0.1665 mL 0.8325 mL 1.6649 mL 4.1624 mL
25 mM 0.1332 mL 0.6660 mL 1.3320 mL 3.3299 mL
30 mM 0.1110 mL 0.5550 mL 1.1100 mL 2.7749 mL
40 mM 0.0832 mL 0.4162 mL 0.8325 mL 2.0812 mL
50 mM 0.0666 mL 0.3330 mL 0.6660 mL 1.6649 mL
60 mM 0.0555 mL 0.2775 mL 0.5550 mL 1.3875 mL
80 mM 0.0416 mL 0.2081 mL 0.4162 mL 1.0406 mL
100 mM 0.0333 mL 0.1665 mL 0.3330 mL 0.8325 mL
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NPPB Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NPPB107254-86-4Chloride ChannelCl− ChannelsInhibitorinhibitorinhibit

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製品名:
NPPB
製品番号:
HY-101012
数量:
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