1. Stem Cell/Wnt
  2. Smo
  3. Purmorphamine

Purmorphamine (Synonyms: Shh Signaling Antagonist VI)

Cat. No.: HY-15108 Purity: 99.56%
Handling Instructions

Purmorphamine (Shh Signaling Antagonist VI) is a smoothened/Smo receptor agonist with an EC50 of 1 μM.

For research use only. We do not sell to patients.

Purmorphamine Chemical Structure

Purmorphamine Chemical Structure

CAS No. : 483367-10-8

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10 mM * 1 mL in DMSO USD 103 In-stock
Estimated Time of Arrival: December 31
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5 mg USD 90 In-stock
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10 mg USD 160 In-stock
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50 mg USD 720 In-stock
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Customer Review

Based on 10 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Purmorphamine purchased from MCE. Usage Cited in: Cancer Med. 2018 Nov;7(11):5704-5715.

    The Hh signaling pathway is involved in DHA induced inhibition of cell proliferation. A and B, CCK8 assay of SKOV3 and SKOV3-IP cells is conducted following treatment with purmorphamine, DHA or a combination of DHA and purmorphamine for 48 h. C and D, SKOV3 and SKOV3‐IP cells are treated with GANT61, DHA, or a combination of DHA and GATN61 for 48 h, while controls are treated with DMSO. CCK8 assay is used to analyze cell viability of SKOV3 and SKOV3-IP cells.
    • Biological Activity

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    • Customer Review


    Purmorphamine (Shh Signaling Antagonist VI) is a smoothened/Smo receptor agonist with an EC50 of 1 μM.

    IC50 & Target

    IC50: 1.5 μM (Smoothened)

    In Vitro

    Purmorphamine (Shh Signaling Antagonist VI; 10, 20 μM) in combination with sirolimus significantly decreases cell numbers according to the MTT assay. Purmorphamine induces up-regulation of alkaline phosphatase activity and expression of RUNX-2 at day 14. Up-regulation of osteocalcin is detected at the 3 and 5 μM doses of purmorphamine on day 14 post-induction. Matrix mineralization remains unchanged in the presence or absence of purmorphamine[1]. Purmorphamine induces STAT3 phosphorylation in mouse ES cell line ES14 and mesenchymal stem cell line C3H10T1/2[2]. Purmorphamine up-regulates the expressionof markers of the osteoblast phenotype-ALP activity and bone-like nodule formationd-in human bonemarrow mesenchymal cells[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.



    Room temperature in continental US; may vary elsewhere.

    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (96.04 mM; Need ultrasonic)

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9208 mL 9.6039 mL 19.2079 mL
    5 mM 0.3842 mL 1.9208 mL 3.8416 mL
    10 mM 0.1921 mL 0.9604 mL 1.9208 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    Cell Assay

    To determine non-toxic doses of the small molecules, 5×105 passaged-3 human MSCs are seeded in each well of a six-well culture plate and incubated in expansion medium (as mentioned above) at 37°Cand 5% CO2. Two days later, the medium is exchanged with osteogenic medium (OM) that consisted of α-MEM supplemented with 10% FBS, 10 nM dexamethasone, 50 μg/mL ascorbic acid 2-phosphate, and 10 mM beta-glycerol phosphate. This OM is supplemented with different concentrations of purmorphamine (1, 3, 5, 10, and 20 μM) and sirolimus (0.1, 1, 10, 100, and 200 nM). The cultures are maintained for an additional two days and then assessed for the presence of viable cells with the MTT assay, by the addition of MTT solution (5 mg/mL in PBS) to the medium at a ratio of 1:5. Cells are then incubated at 37°C and 5% CO2. Two hours later, the medium is removed and 500 μL of DMSO is added to the treated cells in order to dissolve the formazone precipitate. The optical absorption rate is read at 570 nm. Cell viability is calculated as percent value relative to the control group which is only treated with OM.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.


    Purity: 99.89%

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