1. Cell Cycle/DNA Damage
    Epigenetics
    Apoptosis
  2. HDAC
    Apoptosis
  3. Romidepsin

Romidepsin (Synonyms: FK 228; FR 901228; NSC 630176)

Cat. No.: HY-15149 Purity: 99.98%
Handling Instructions

Romidepsin (FK 228) is a Histone deacetylase (HDAC) inhibitor with anti-tumor activities. Romidepsin (FK 228) inhibits HDAC1, HDAC2, HDAC4, and HDAC6 with IC50s of 36 nM, 47 nM, 510 nM and 1.4 μM, respectively. Romidepsin (FK 228) is produced by Chromobacterium violaceum, induces cell G2/M phase arrest and apoptosis.

For research use only. We do not sell to patients.

Romidepsin Chemical Structure

Romidepsin Chemical Structure

CAS No. : 128517-07-7

Size Price Stock Quantity
1 mg USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 300 In-stock
Estimated Time of Arrival: December 31
10 mg USD 540 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 16 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Romidepsin purchased from MCE. Usage Cited in: Neurosci Lett. 2017 May 12;653:12-18.

    Romidepsin increases histone acetylation in SH-SY5Y neuroblastoma cells. Western blots of pAcH3 and β-actin levels in SH-SY5Y cells cultured for 24 h with 0-40 nM of Romidepsin. 20 nM and 40 nM Romidepsin significantly increase the levels of pAcH3 relative to control, which is measured by densitometric quantification of pAcH3 protein levels relative to β-actin loading control.

    Romidepsin purchased from MCE. Usage Cited in: PLoS Pathog. 2018 Sep 13;14(9):e1007267. 

    Immunoblot analysis for vIL-6, ORF45, or actin is performed on lysates from BCBL-1 cells treated with NaB+TPA, Romidepsin or Panobinostat.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Romidepsin (FK 228) is a Histone deacetylase (HDAC) inhibitor with anti-tumor activities. Romidepsin (FK 228) inhibits HDAC1, HDAC2, HDAC4, and HDAC6 with IC50s of 36 nM, 47 nM, 510 nM and 1.4 μM, respectively[1]. Romidepsin (FK 228) is produced by Chromobacterium violaceum, induces cell G2/M phase arrest and apoptosis[2].

    IC50 & Target[1]

    HDAC1

    36 nM (IC50)

    HDAC2

    47 nM (IC50)

    HDAC4

    510 nM (IC50)

    HDAC6

    14000 nM (IC50)

    In Vitro

    Romidepsin (0-72 hours; 0-80 nM) inhibits proliferation of HCC cells in dose-dependent manner[2].
    Romidepsin (0-48 hours; 0-60 nM) leads to a time- and dose-dependent induction of cell cycle arrest in the G2/M phase in HCC cells[2].
    Romidepsin (0-48 hours; 0-60 nM) promotesapoptosis in HCC cells, increases c-caspase-3, c-caspase-9, and c-PARP protein expression[2].

    Cell Proliferation Assay[2]

    Cell Line: HCC cells
    Concentration: 0 nM; 10 nM; 20 nM; 30 nM; 40 nM; 50 nM; 60 nM; 70 nM; 80 nM
    Incubation Time: 0 hours; 12 hours; 24 hours; 48 hours; 72 hours
    Result: Inhibited HCC cells proliferation.

    Cell Cycle Analysis[2]

    Cell Line: HCC cells
    Concentration: 0 nM; 15 nM; 30 nM; 60 nM
    Incubation Time: 12 hours;24 hours; 48 hours
    Result: Caused a G2/M arrest.

    Western Blot Analysis[2]

    Cell Line: HCC cells
    Concentration: 0 nM; 15 nM; 30 nM; 60 nM
    Incubation Time: 12 hours;24 hours; 48 hours
    Result: Increaesd c-caspase-3, c-caspase-9, and c-PARP expression in HCC cells.
    In Vivo

    Romidepsin (intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days) inhibited the tumor growth, reveals a higher expression of p-cdc25C, ki67, c-caspase-3 and c-PARP, and a lower expression of Ki-67 in Romidepsin treated tumors [2].

    Animal Model: Nude mice with Huh7 cells[2]
    Dosage: 0.5 and 1 mg/kg
    Administration: Intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days
    Result: Suppressed tumor growth in mouse xenograft models.
    Clinical Trial
    Molecular Weight

    540.70

    Formula

    C₂₄H₃₆N₄O₆S₂

    CAS No.

    128517-07-7

    SMILES

    C/C=C(NC([[email protected]@H](CSSCC/C=C/[[email protected]@H](O1)CC2=O)NC([[email protected]@H](C(C)C)N2)=O)=O)/C(N[[email protected]@H](C(C)C)C1=O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (92.47 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8495 mL 9.2473 mL 18.4945 mL
    5 mM 0.3699 mL 1.8495 mL 3.6989 mL
    10 mM 0.1849 mL 0.9247 mL 1.8495 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (4.62 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.62 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.98%

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    Product Name:
    Romidepsin
    Cat. No.:
    HY-15149
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