SAR7334 hydrochloride
Based on 21 publication(s) in Google Scholar
SAR7334 hydrochloride is a potent and specific TRPC6 inhibitor, inhibiting TRPC6 currents with IC50 of 7.9 nM.
For research use only. We do not sell to patients.
- Purity: 96.04%
- CAS No.: 1333207-63-8
- Formula: C21H24Cl3N3O
- Molecular Weight:440.79
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) SAR7334 hydrochloride
More- Mol Ther. 2022 Apr 6;30(4):1754-1774. [Abstract]
- Cell Death Dis. 2020 Mar 5;11(3):170. [Abstract]
- Cell Death Dis. 2018 Oct 3;9(10):1015. [Abstract]
- Virulence. 2025 Dec;16(1):2490208. [Abstract]
- Cells. 2020 Jan 14;9(1):202. [Abstract]
- Eur J Pharmacol. 2022 Oct 15:933:175254. [Abstract]
- J Physiol. 2025 Aug 28. [Abstract]
- J Physiol. 2024 Feb 10:10.1113/JP286118. [Abstract]
- FASEB J. 2022 Oct;36(10):e22527. [Abstract]
- J Mol Med (Berl). 2018 Jul;96(7):631-644. [Abstract]
- Biochim Biophys Acta. 2017 Jun 16;1863(9):2342-2354. [Abstract]
- Cell Calcium. 2019 Jan:77:8-19. [Abstract]
- Biochim Biophys Acta. 2017 Jul 25;1863(11):2964-2972. [Abstract]
- Exp Cell Res. 2022 Dec 1;421(1):113374. [Abstract]
- Food Chem Toxicol. 2019 Jul:129:281-290. [Abstract]
- J Diabetes Res. 2020 Sep 16;2020:3634974. [Abstract]
- Biochem Biophys Res Commun. 2025 Nov 1:787:152776. [Abstract]
- Heart Vessels. 2020 Dec;35(12):1755-1765. [Abstract]
- The Medical College of Wisconsin. 2025.
- bioRxiv. 2024 Dec 23:2024.12.23.629519. [Abstract]
- Research Square Preprint. 2024 Mar 28.
Customer Validation & Images
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Biological Activity
IC50: 7.9 nM (TRPC6 currents)[3]
SAR7334 inhibits TRPC6, TRPC3 and TRPC7-mediated Ca2+ influx into cells with IC50s of 9.5, 282 and 226 nM[1][2][3], whereas TRPC4 and TRPC5-mediated Ca2+ entry is not affected. SAR7334 (1 μM) results in a major block of the Ang II-evoked calcium influx in the podocytes[1]. SAR7334 (1 μM) has negligible effect on SOCE[2]. SAR7334 dose-dependently reduces TRPC6 currents with an IC50 of 7.9 nM. SAR7334 (100 nM) substantially reduces TRPC6 currents[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1333207-63-8
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Appearance Solid
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Molecular Weight 440.79
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Formula C21H24Cl3N3O
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Color White to yellow
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SMILES
N[C@@H](C1)CCCN1[C@@H]2CC3=CC=CC=C3[C@H]2OC4=CC=C(C#N)C=C4Cl.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (21)
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Journal Impact Factor
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Most Recent
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Mol Ther
2022 Apr 6;30(4):1754-1774. PMID: 35077860 -
Cell Death Dis
TRPC6-dependent Ca2+ signaling mediates airway inflammation in response to oxidative stress via ERK pathway. [Abstract]2020 Mar 5;11(3):170. PMID: 32139669 -
Cell Death Dis
Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. [Abstract]2018 Oct 3;9(10):1015. PMID: 30282964
SAR7334 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 3;9(10):1015. [Abstract]
HK-2 cells are treated with different concentrations of SAR7334 for 12 h. Representative western blot images and the relative quantification of LC3-II are shown.
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Virulence
2025 Dec;16(1):2490208. PMID: 40202859 -
Cells
2020 Jan 14;9(1):202. PMID: 31947602 -
Eur J Pharmacol
Puerarin ameliorates myocardial remodeling of spontaneously hypertensive rats through inhibiting TRPC6-CaN-NFATc3 pathway. [Abstract]2022 Oct 15:933:175254. PMID: 36087696 -
J Physiol
GsMTx-4 inhibits the exercise pressor reflex and the muscle mechanoreflex primarily through TRPC inhibition. [Abstract]2025 Aug 28. PMID: 40876864 -
J Physiol
Inhibition and potentiation of the exercise pressor reflex by pharmacological modulation of TRPC6 in male rats. [Abstract]2024 Feb 10:10.1113/JP286118. PMID: 38340081 -
FASEB J
TRPC6 inhibits renal tubular epithelial cell pyroptosis through regulating zinc influx and alleviates renal ischemia-reperfusion injury. [Abstract]2022 Oct;36(10):e22527. PMID: 36036542 -
J Mol Med (Berl)
2018 Jul;96(7):631-644. PMID: 29785489 -
Biochim Biophys Acta
Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factors. [Abstract]2017 Jun 16;1863(9):2342-2354. PMID: 28629718 -
Cell Calcium
Downregulation of TRPC6 expression is a critical molecular event during FK506 treatment for overactive bladder. [Abstract]2019 Jan:77:8-19. PMID: 30476735 -
Biochim Biophys Acta
An abnormal TRPV4-related cytosolic Ca2+ rise in response to uniaxial stretch in induced pluripotent stem cells-derived cardiomyocytes from dilated cardiomyopathy patients. [Abstract]2017 Jul 25;1863(11):2964-2972. PMID: 28754452 -
Exp Cell Res
Inhibition of TRPC6 suppressed TGFβ-induced fibroblast-myofibroblast transdifferentiation in renal interstitial NRK-49F cells. [Abstract]2022 Dec 1;421(1):113374. PMID: 36206825 -
Food Chem Toxicol
Activation of TRPC6 channels contributes to (+)-conocarpan-induced apoptotic cell death in HK-2 cells. [Abstract]2019 Jul:129:281-290. PMID: 31054997 -
J Diabetes Res
Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes. [Abstract]2020 Sep 16;2020:3634974. PMID: 33015191 -
Biochem Biophys Res Commun
Non-Ser36 phosphorylated p66Shc inhibits TRPC-dependent and TRPC-independent calcium influx in vascular smooth muscle cells. [Abstract]2025 Nov 1:787:152776. PMID: 41086682 -
Heart Vessels
TRPC6 participates in the development of blood pressure variability increase in sino-aortic denervated rats. [Abstract]2020 Dec;35(12):1755-1765. PMID: 32844288 -
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bioRxiv
Angiotensin II elicits robust calcium oscillations coordinated within juxtaglomerular cell clusters to suppress renin secretion. [Abstract]2024 Dec 23:2024.12.23.629519. PMID: 39763801 -
Solvent & Solubility
DMSO : 100 mg/mL (226.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (113.43 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
Adult male (6 months old) spontaneously hypertensive rats (SHR) are treated on two consecutive days. On day one, the animals receive 1 mL/kg vehicle by oral gavage. After 24 h, rats receive either vehicle alone or are treated with 10 mg/kg SAR7334. Telemetric measurement of BP is performed. In brief, a telemetric device (TL11M2-C50-PXT PMP) is placed between the aorta and the vena cava and the catheter tip of the transmitter is inserted into the aorta. Systolic BP, diastolic BP and heart rate are acquired continuously at a sampling rate of 500 Hz and data are stored as 5 min averages. Mean arterial pressure is calculated from systolic and diastolic pressure and low-pass filtered using the fast Fourier transform function of the vendor software for better visualization of time-dependent BP variations. For statistical analysis, raw data are averaged over a 6 h period starting 2 h after application of vehicle or SAR7334. This interval corresponds to the maximal plasma levels of SAR7334. Baseline data are sampled over the same time interval on the day before treatment.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ilatovskaya DV, et al. The Role of Angiotensin II in Glomerular Volume Dynamics and Podocyte Calcium Handling. Sci Rep. 2017 Mar 22;7(1):299. [Content Brief]
[2]. Chauvet S, et al. Pharmacological Characterization of the Native Store-Operated Calcium Channels of Cortical Neurons from Embryonic Mouse Brain. Front Pharmacol. 2016 Dec 12;7:486. [Content Brief]
[3]. Maier T, et al. Discovery and pharmacological characterization of a novel potent inhibitor of diacylglycerol-sensitive TRPC cation channels. Br J Pharmacol. 2015 Jul;172(14):3650-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.2687 mL | 11.3433 mL | 22.6865 mL | 56.7163 mL |
| 5 mM | 0.4537 mL | 2.2687 mL | 4.5373 mL | 11.3433 mL | |
| 10 mM | 0.2269 mL | 1.1343 mL | 2.2687 mL | 5.6716 mL | |
| 15 mM | 0.1512 mL | 0.7562 mL | 1.5124 mL | 3.7811 mL | |
| 20 mM | 0.1134 mL | 0.5672 mL | 1.1343 mL | 2.8358 mL | |
| 25 mM | 0.0907 mL | 0.4537 mL | 0.9075 mL | 2.2687 mL | |
| 30 mM | 0.0756 mL | 0.3781 mL | 0.7562 mL | 1.8905 mL | |
| 40 mM | 0.0567 mL | 0.2836 mL | 0.5672 mL | 1.4179 mL | |
| 50 mM | 0.0454 mL | 0.2269 mL | 0.4537 mL | 1.1343 mL | |
| 60 mM | 0.0378 mL | 0.1891 mL | 0.3781 mL | 0.9453 mL | |
| 80 mM | 0.0284 mL | 0.1418 mL | 0.2836 mL | 0.7090 mL | |
| 100 mM | 0.0227 mL | 0.1134 mL | 0.2269 mL | 0.5672 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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