SW033291

Name: SW033291
Brand: MedChemExpress
SKU: HY-16968
Rating: 5.0 (7 reviews)

Cat. No.: HY-16968 Purity: 99.36%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

SW033291 is a potent and high-affinity inhibitor of 15-PGDH with a K_i of 0.1 nM. SW033291 increases prostaglandin PGE2 levels in bone marrow and other tissues. SW033291 also promotes tissue regeneration.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 459147-39-8

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 7 publication(s) in Google Scholar

Other Forms of SW033291:

(R)-SW033291 견적 받기

7 Publications Citing Use of MCE SW033291

•J Biochem. 2023 Mar 31;173(4):293-305. [Abstract]

Cell Proliferation/Viability Assay

Cell Imaging/Staining

RT-PCR

IHC

: SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]; Cell viability of MDSCs in GM under different concentration of SW033291 (20, 50, 100, 500, 1000 nM) treatment.

: SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]; LIVE/DEAD staining of MDSCs cultured in DM and GM treated with different concentration of SW033291 (20, 50, 100, 500, 1000 nM) on day 1 and day 3.

: SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]; qPCR analysis of the expression of myogenic differentiation-related genes in MDSCs cultured in DM (top row) and GM (bottom row) under 100 nM SW033291 treatment on days 3 and 7.

: SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]; Western blot analysis of the protein expression of myogenic markers in MDSCs cultured in DM and GM under 100 nM SW033291 treatment on day 3.

: SW033291 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]; Cellular immunochemistry image of MHC in MDSCs cultured in DM and GM under 100 nM SW033291 treatment on day 7.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

Ki: 0.1 nM (15-PGDH)^[1]

In Vitro

Treating cells with SW033291 decreases cellular 15-PGDH enzyme activity by 85%. SW033291 inhibition of 15-PGDH was non-competitive versus PGE2 over concentrations up to 40 μM PGE2. Treatment of A549 cells with SW033291 increases PGE2 levels by 3.5-fold at 500 nM, with an EC₅₀ 50 at approximately 75 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SW033291 (10 mg/kg; intraperitoneal injection; twice daily; for 3 days; C57BL/6J mice) treatment for three consecutive days shows significant benefits, including a doubling of peripheral neutrophil counts, a 65% increase in marrow SKL cells, and a 71% increase in marrow SLAM cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J mice^[1]
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection; twice daily; for 3 days ( for 5 doses)
Result:	Showed significant benefits, including a doubling of peripheral neutrophil counts, a 65% increase in marrow SKL cells, and a 71% increase in marrow SLAM cells.

분자량

412.59

화학식

C₂₁H₂₀N₂OS₃

CAS No.

459147-39-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=C(S(CCCC)=O)SC2=NC(C3=CC=CS3)=CC(C4=CC=CC=C4)=C21

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : 33.33 mg/mL (80.78 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (60.59 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4237 mL	12.1186 mL	24.2371 mL
5 mM	0.4847 mL	2.4237 mL	4.8474 mL
10 mM	0.2424 mL	1.2119 mL	2.4237 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.06 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.06 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.06 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.36%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) HNMR (270 KB) RP-HPLC (260 KB) MS (229 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang Y, et al. TISSUE REGENERATION. Inhibition of the prostaglandin-degrading enzyme 15-PGDH potentiates tissue regeneration. Science. 2015 Jun 12;348(6240):aaa2340. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.4237 mL	12.1186 mL	24.2371 mL	60.5928 mL
	5 mM	0.4847 mL	2.4237 mL	4.8474 mL	12.1186 mL
	10 mM	0.2424 mL	1.2119 mL	2.4237 mL	6.0593 mL
	15 mM	0.1616 mL	0.8079 mL	1.6158 mL	4.0395 mL
	20 mM	0.1212 mL	0.6059 mL	1.2119 mL	3.0296 mL
	25 mM	0.0969 mL	0.4847 mL	0.9695 mL	2.4237 mL
	30 mM	0.0808 mL	0.4040 mL	0.8079 mL	2.0198 mL
	40 mM	0.0606 mL	0.3030 mL	0.6059 mL	1.5148 mL
	50 mM	0.0485 mL	0.2424 mL	0.4847 mL	1.2119 mL
	60 mM	0.0404 mL	0.2020 mL	0.4040 mL	1.0099 mL
DMSO	80 mM	0.0303 mL	0.1515 mL	0.3030 mL	0.7574 mL

SW033291 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SW033291459147-39-8SW 033291SW-03329115-PGDH15-Hydroxyprostaglandin dehydrogenasePeripheralneutrophilknockoutprostaglandinPGE2hematopoieticbonemarrowInhibitorinhibitorinhibit

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SW033291

고객리뷰

Other Forms of SW033291:

SW033291 Related Antibodies

7 Publications Citing Use of MCE SW033291

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

SW033291 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

SW033291 Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report