Texas Red (Synonyms: Sulforhodamine 101; SR101)

Name: Texas Red
Brand: MedChemExpress
SKU: HY-101878
Rating: 5.0 (5 reviews)

Cat. No.: HY-101878 Pureza: 99.72%: Ficha de datos
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Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.

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No. CAS : 60311-02-6

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25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
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Based on 5 publication(s) in Google Scholar

Other Forms of Texas Red:

Texas Red (solution) Obtener un presupuesto
Texas Red (Standard) Obtener un presupuesto

5 Publications Citing Use of MCE Texas Red

Bio/Physico-chemical Assay

Cell Imaging/Staining

: Texas Red purchased from MedChemExpress. Usage Cited in: Neuron. 2022 Sep 21;110(18):2984-2999.e8. [Abstract]; The astrocyte-specific dye Texas Red (Sulforhodamine 101; SR101) (1 μM; 15 min; 37 ℃) was used for two-photon Ca²⁺ imaging of hippocampal slices.

: Texas Red purchased from MedChemExpress. Usage Cited in: Neuropharmacology. 2022 Oct 1:217:109191. [Abstract]; Example image of a patch-clamp recorded astrocyte labeled with Texas Red (Sulforhodamine 101; SR101) (5 μM; 35 min; 34 ℃) in the alveus of the hippocampal CA1 region.

: Texas Red purchased from MedChemExpress. Usage Cited in: Neuropharmacology. 2022 Oct 1:217:109191. [Abstract]; Example images of astrocytes in hippocampal slices labeled with Texas Red (Sulforhodamine 101; SR101) (5 μM; 35 min; 34 ℃) and filled with Fluo-4, a calcium indicator. Arrows indicate astrocytes co-labeled with Texas Red (Sulforhodamine 101; SR101) and Fluo-4.

: Texas Red purchased from MedChemExpress. Usage Cited in: J Biomed Res. 2022 Aug 10;36(5):358-367. [Abstract]; The trajectory of astrocytic Ca²⁺ signal-weighted centroid from C57BL/6J mice. The grey cellular surface was reconstructed from Texas Red (Sulforhodamine 101; SR101) (5 mM; i.v.; 1 h) labeling. The overlapped red trace was the projection of signal centroid.

: Texas Red purchased from MedChemExpress. Usage Cited in: Glia. 2021 Feb;69(2):281-295. [Abstract]; Representative fluorescence images showed Na⁺-insensitive fluorescent dye Texas Red (Sulforhodamine 101; SR101) (1 μM; 30 min; 37 ℃) trapped inside astroglial cytoplasm and counterstained with Hoechst 33342.

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Descripciòn

In Vitro

Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence^[3].
For cell staining.
1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34°C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

606.71

Fòrmula

C₃₁H₃₀N₂O₇S₂

No. CAS

60311-02-6

Appearance

Solid

Color

Green to dark green

Emission (Em)

605

Excitation (Ex)

586

SMILES

O=S(C1=CC=C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)C(S(=O)([O-])=O)=C1)(O)=O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvente y solubilidad

In Vitro:

DMSO : 31.82 mg/mL (52.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 8.33 mg/mL (13.73 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6482 mL	8.2412 mL	16.4823 mL
5 mM	0.3296 mL	1.6482 mL	3.2965 mL
10 mM	0.1648 mL	0.8241 mL	1.6482 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.72%

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Ficha de datos (284 KB) SDS (393 KB)

COA (261 KB) LCMS (93 KB)

Instrucciones de manejo (2659 KB)

Dyeing Example

Description: Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence.
Method: For cell staining.
1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34◦C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.

Referencias

[1]. Axel Nimmerjahn, et al. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2004 Oct;1(1):31-7. [Content Brief]

[2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9. [Content Brief]

[3]. Wang T, et al. Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. Neuropharmacology. 2022 Oct 1;217:109191. [Content Brief]

Ensayo celular ^[1]	Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Texas Red for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier^[1]. MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Administraciòn de animales ^[1]	For in vivo imaging, Texas Red (Sulforhodamine 101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Texas Red injection over the tail vein (10 mg/mL) has been reported to be successful^[1]. MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Referencias	[1]. Axel Nimmerjahn, et al. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2004 Oct;1(1):31-7. [Content Brief] [2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9. [Content Brief] [3]. Wang T, et al. Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. Neuropharmacology. 2022 Oct 1;217:109191. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.6482 mL	8.2412 mL	16.4823 mL	41.2058 mL
	5 mM	0.3296 mL	1.6482 mL	3.2965 mL	8.2412 mL
	10 mM	0.1648 mL	0.8241 mL	1.6482 mL	4.1206 mL
DMSO	15 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7471 mL
	20 mM	0.0824 mL	0.4121 mL	0.8241 mL	2.0603 mL
	25 mM	0.0659 mL	0.3296 mL	0.6593 mL	1.6482 mL
	30 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3735 mL
	40 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0301 mL
	50 mM	0.0330 mL	0.1648 mL	0.3296 mL	0.8241 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Texas Red Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

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Texas Red (Synonyms: Sulforhodamine 101; SR101)

Revisión del cliente

Other Forms of Texas Red:

Texas Red Related Antibodies

5 Publications Citing Use of MCE Texas Red

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Protocolo

Pureza y Documentación

Referencias

Revisión del cliente

Texas Red Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Texas Red Related Classifications

Keywords:

Productos vistos recientemente:

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