1. MAPK/ERK Pathway
  2. Mixed Lineage Kinase


Cat. No.: HY-101524
Handling Instructions

TC13172 is a mixed lineage kinase domain-like protein (MLKL) inhibitor with an EC50 value of 2 nM for HT-29 cells.

For research use only. We do not sell to patients.

TC13172 Chemical Structure

TC13172 Chemical Structure

CAS No. : 2093393-05-4

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


TC13172 is a mixed lineage kinase domain-like protein (MLKL) inhibitor with an EC50 value of 2 nM for HT-29 cells.

IC50 & Target

EC50: 2±0.6 nM (MLKL, in HT-29 cells)[1].

In Vitro

The anti-necroptosis potency of TC13172 is evaluated in the HT-29 cell line, the EC50 value is 2±0.6 nM. TC13172 has an inhibition potency of 2 nM against cell necroptosis. TC13172 inhibits MLKL by directly binding to Cys-86. TC13172 does not disrupt the phosphorylation of MLKL, but do decrease the level of MLKL in the membrane phase, demonstrating that these MLKL inhibitors block the translocation of MLKL to the cell membrane, thereby protecting cells from necroptosis[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5747 mL 12.8733 mL 25.7467 mL
5 mM 0.5149 mL 2.5747 mL 5.1493 mL
10 mM 0.2575 mL 1.2873 mL 2.5747 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Human colorectal adenocarcinoma (HT)-29 cells are incubated with compound 12 (1 mM) or DMSO for 2 h. For the binding competition experiment samples, cells are pre-incubated with 5 mM NSA or 100 nM TC13172 for 2 h, 1 mM compound 12 is then added and incubated for an additional 2 h. The click reaction (Biotin-C2H4-N3 10 mM, TBTA 10 mM, CuSO4 50 mM, Sodium ascorbate 50 mM) is carried out with cell lysates for 2 h. The biotinmodified proteins are enriched and analysed by western blotting using antibodies against flag and GAPDH[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









Please store the product under the recommended conditions in the Certificate of Analysis.


Room temperature in continental US; may vary elsewhere

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Cat. No.: HY-101524