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  4. TMRE

TMRE  (Synonyms: Tetramethylrhodamine ethyl ester perchlorate)

製品番号: HY-D0985A 純度: 98.49%
COA 取扱説明書 Technical Support

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

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CAS 番号 : 115532-52-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 42 在庫あり
Solution
10 mM * 1 mL in DMSO USD 42 在庫あり
Solid
5 mg $37 在庫あり
10 mg $59 在庫あり
25 mg $118 在庫あり
50 mg $190 在庫あり
100 mg $304 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 75 publication(s) in Google Scholar

Other Forms of TMRE:

TMRE 関連抗体

Top Publications Citing Use of Products

顧客検証

IF

    TMRE purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Jun 26;17(11):2703-2717.  [Abstract]

    When the mitochondrial membrane potential (ΔΨm) is high, TMRE gathers in the mitochondria and produces red-orange fluorescence. HCT116 cells are seeded in 96‐well plates and treated with Tagitinin C. Then cells are incubated for 30 minutes at 37 °C with TMRE (1 µM) in PBS. The excitation wavelength is 540 nm, and the emission wavelength is 595 nm.

    TMRE purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2021 Sep 15;221:112425.  [Abstract]

    AML-12 cells were treated with TDBPP, then TMRE (100 nM) was added to each well. Cells were stained for 30 min. The red fluorescence stained by TMRE represented mitochondria.

    TMRE purchased from MedChemExpress. Usage Cited in: FASEB J. 2020 Apr;34(4):5740-5753.  [Abstract]

    Cells were rinsed with D-PBS, and then incubated with TMRE (50 nM) at 37°C for 20 minutes.

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    製品説明

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    体外実験

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1.Preparation of TMRE working solution
    1.1Preparation of the stock solution
    Dissolve TMRE in DMSO to obtain 5 mM of stock solution.
    1.2 Preparation of TMRE working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
    Note: Please adjust the concentration of TMRE working solution according to the actual situation.
    2. Cell staining (suspended cells)
    2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
    2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    3. Cell staining (adherent cells)
    3.1 Culture adherent cells on sterile coverslips.
    3.2 Remove the coverslip from the medium and aspirate excess medium.
    3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
    3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
    Note: If detection by flow cytometry, cells need to be resuspended before staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMRE 関連抗体
    分子量

    514.95

    分子式

    C26H27ClN2O7
    Emission (Em)

    576
    Excitation (Ex)

    550
    CAS 番号
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OCC.O=Cl(=O)([O-])=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 27.78 mg/mL (53.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9419 mL 9.7097 mL 19.4194 mL
    5 mM 0.3884 mL 1.9419 mL 3.8839 mL
    10 mM 0.1942 mL 0.9710 mL 1.9419 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

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    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 98.49%

    COA (255 KB) HNMR (254 KB) RP-HPLC (252 KB) LCMS (92 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献
    細胞実験
    [1]

    The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9419 mL 9.7097 mL 19.4194 mL 48.5484 mL
    5 mM 0.3884 mL 1.9419 mL 3.8839 mL 9.7097 mL
    10 mM 0.1942 mL 0.9710 mL 1.9419 mL 4.8548 mL
    15 mM 0.1295 mL 0.6473 mL 1.2946 mL 3.2366 mL
    20 mM 0.0971 mL 0.4855 mL 0.9710 mL 2.4274 mL
    25 mM 0.0777 mL 0.3884 mL 0.7768 mL 1.9419 mL
    30 mM 0.0647 mL 0.3237 mL 0.6473 mL 1.6183 mL
    40 mM 0.0485 mL 0.2427 mL 0.4855 mL 1.2137 mL
    50 mM 0.0388 mL 0.1942 mL 0.3884 mL 0.9710 mL
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    TMRE Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMRE115532-52-0Tetramethylrhodamine ethyl esterFluorescent DyeInhibitorinhibitorinhibit

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    製品名:
    TMRE
    製品番号:
    HY-D0985A
    数量:
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