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  4. TMRE

TMRE  (Synonyms: Tetramethylrhodamine ethyl ester perchlorate)

Cat. No.: HY-D0985A Purity: 98.49%
COA
SDS
Handling Instructions Technical Support

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 115532-52-0

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 75 publication(s) in Google Scholar

Other Forms of TMRE:

TMRE Related Antibodies

Top Publications Citing Use of Products

75 Publications Citing Use of MCE TMRE

IF

    TMRE purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Jun 26;17(11):2703-2717.  [Abstract]

    When the mitochondrial membrane potential (ΔΨm) is high, TMRE gathers in the mitochondria and produces red-orange fluorescence. HCT116 cells are seeded in 96‐well plates and treated with Tagitinin C. Then cells are incubated for 30 minutes at 37 °C with TMRE (1 µM) in PBS. The excitation wavelength is 540 nm, and the emission wavelength is 595 nm.

    TMRE purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2021 Sep 15;221:112425.  [Abstract]

    AML-12 cells were treated with TDBPP, then TMRE (100 nM) was added to each well. Cells were stained for 30 min. The red fluorescence stained by TMRE represented mitochondria.

    TMRE purchased from MedChemExpress. Usage Cited in: FASEB J. 2020 Apr;34(4):5740-5753.  [Abstract]

    Cells were rinsed with D-PBS, and then incubated with TMRE (50 nM) at 37°C for 20 minutes.

    • Biological Activity

    • Protocol

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1.Preparation of TMRE working solution
    1.1Preparation of the stock solution
    Dissolve TMRE in DMSO to obtain 5 mM of stock solution.
    1.2 Preparation of TMRE working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
    Note: Please adjust the concentration of TMRE working solution according to the actual situation.
    2. Cell staining (suspended cells)
    2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
    2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    3. Cell staining (adherent cells)
    3.1 Culture adherent cells on sterile coverslips.
    3.2 Remove the coverslip from the medium and aspirate excess medium.
    3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
    3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
    Note: If detection by flow cytometry, cells need to be resuspended before staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMRE Related Antibodies
    분자량

    514.95

    화학식

    C26H27ClN2O7
    CAS No.
    Appearance

    Solid

    Color

    Brown to black

    Emission (Em)

    576
    Excitation (Ex)

    550
    SMILES

    O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OCC.O=Cl(=O)([O-])=O
    선적

    Room temperature in continental US; may vary elsewhere.
    보관

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    용액&용해도
    In Vitro: 

    DMSO : 27.78 mg/mL (53.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9419 mL 9.7097 mL 19.4194 mL
    5 mM 0.3884 mL 1.9419 mL 3.8839 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • 몰농도 계산기

    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    순도&문서

    Purity: 98.49%

    SDS (393 KB)

    COA (255 KB) HNMR (254 KB) RP-HPLC (252 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay 
    [1]

    The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].

    MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9419 mL 9.7097 mL 19.4194 mL 48.5484 mL
    5 mM 0.3884 mL 1.9419 mL 3.8839 mL 9.7097 mL
    10 mM 0.1942 mL 0.9710 mL 1.9419 mL 4.8548 mL
    15 mM 0.1295 mL 0.6473 mL 1.2946 mL 3.2366 mL
    20 mM 0.0971 mL 0.4855 mL 0.9710 mL 2.4274 mL
    25 mM 0.0777 mL 0.3884 mL 0.7768 mL 1.9419 mL
    30 mM 0.0647 mL 0.3237 mL 0.6473 mL 1.6183 mL
    40 mM 0.0485 mL 0.2427 mL 0.4855 mL 1.2137 mL
    50 mM 0.0388 mL 0.1942 mL 0.3884 mL 0.9710 mL
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    TMRE Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMRE115532-52-0Tetramethylrhodamine ethyl esterFluorescent DyeInhibitorinhibitorinhibit

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    상품명:
    TMRE
    Cat. No.:
    HY-D0985A
    수량:
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    SAFETY DATA SHEET (SDS)

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